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Biomedical subjects

M Ueda

Publications and source records attributed to M Ueda.

At least 991 records · Page 55Linked to original sources
PubMed1991

[Complement fragments in patients with bronchial asthma].

In this study, we investigated the pharmacological reactions induced by ibudilast to the complement system with the aim of clarifying the functional relation of the complement system to allergic reactions and pathology in patients with bronchial asthma. Complement hemolytic activities (CH50 and ACH50), complement profile, anaphylatoxins (C3a and C5a) and complement fragments (Bb, iC3b and C4d) were measured in 20 patients with bronchial asthma. One of antiasthmatic activities induced by ibudilast was concluded to be brought about though inactivation of the alternative complement pathway working on type III allergic reaction. Ibudilast increased the complement fragment Bb in the patients' plasma with the fairly controlled bronchial asthma. This increase in circulating Bb was suspected to be a result of inactivation of intermediate complement complexes, for example C3b.Bb.P, because the amounts in plasma of C3 and C5 showed no changes, while those of factor, B, P, H and I were decreased by ibudilast administration in patients with fairly controlled bronchial asthma. This antiasthmatic ability of ibudilast was restrained in those patients whose peripheral leukocytopenia was advanced before ibudilast administration, and in those whom ibudilast did not provoke an increase in the plasma level of iC3b, or did not prevent the serum level of C5 from increasing. In those unfairly controlled cases, enough anaphylatoxins, especially C5a might be produced to make the margination of peripheral neutrophils to the lung and increase CR3 on neutrophils binding with iC3b.

Adult↗
PubMed1991

[Elevated Clq-bearing immune complexes in hemophiliacs with viral infections].

One hundred hemophilia A and 30 hemophilia B patients who had been treated with non-heated and heated factor VIII or prothrombin complex concentrates were examined by immunological tests including Clq-bearing immune complexes assay. Antibodies to human immunodeficiency virus type 1 (HIV-1), hepatitis B virus (HBV), hepatitis C virus (HCV) and human parvovirus B19 (B19) were analyzed by Western blotting, enzyme immunoassay, passive hemagglutination or radio-immunoassay. Clq-bearing immune complexes were assayed by a monoclonal anti-Clq ELISA system (Immunomedics). Seropositivity to HIV-1, HBV, HCV, and B19 was 56.9%, 87.7%, 79.2% and 100% respectively. Clq-bearing immune complexes were positive in 109 of the 130 patients (83.8%). The positivity and the levels were extremely higher than those in normal individuals. Clq-bearing immune complex levels in patient positive for HIV-1, HCV, or HBV were higher than those in the negative group (HIV: P less than 0.001, HCV: P less than 0.005, HBV: P less than 0.05). When the patients were divided into four groups according to seropositivity to HIV-1 and/or HCV, Clq-bearing immune complex levels were the highest in the group positive for both antibodies, and the lowest in the group negative for both antibodies. These results suggested that each viral infection influences the formation of immune complexes and repeated viral infection increased the level of Clq-bearing immune complexes in these patients.

Adolescent↗
PubMed1991

[Clinical evaluation of arterial tonometry for noninvasive, continuous blood pressure monitoring during anesthesia].

A new continuous, non-invasive arterial blood pressure monitoring system based on arterial tonometry was currently developed. A sensor of this system consists of an array of 15 piezoresistive pressure transducers. An automatic sensor positioning system ensure that at least one of the 15 transducers is positioned on the center of the pneumatically flattened, but not occluded, portion of the artery. Accuracy, reliability and clinical acceptance of this system were evaluated by comparing tonometric blood pressure (TBP) measurements with intra-arterial blood pressure (IBP) measurements in 70 normotensive anesthetized patients (normotensive group) and 21 hypotensive anesthetized patients (hypotensive group). IBP was measured in either the right or left radial artery by a Gould P23XL calibrated transducer while TBP was measured at the radial artery of the other side. The outputs of the two systems were recorded on a PCM tape recorder for later analysis. The TBP waveform was similar to the IBP waveform. A significantly high correlation existed between the two methods for systolic, mean and diastolic measurements. Magnitudes of bias (mean difference between the two methods) of the normotensive group were -0.4 +/- 5.9 (mean +/- SD) mmHg for systolic, -0.1 +/- 4.7 mmHg for mean and 0.0 +/- 5.4 mmHg for diastolic measurements and those of the hypotensive group were -0.7 +/- 6.6 mmHg for systolic, 0.7 +/- 5.0 mmHg for mean and 2.8 +/- 5.8 mmHg for diastolic measurements. The "limits of agreement" (mean difference +/- 2SD of bias) for both groups were within the range which would be acceptable for clinical setting. These results indicate that tonometry provides accurate, reliable and real-time blood pressure measurements together with continuous display of the pressure waveform for wide range of blood pressure. It was concluded that this system may replace the conventional invasive intra-arterial blood pressure monitoring system. This system is especially useful if it is combined with pulse oximetry and capnography.

Adolescent↗
PubMed1991

Molecular diversity of erythrocyte calpastatin.

According to the difference of molecular masses estimated by SDS-polyacrylamide gel electrophoresis, calpastatins are classified into two types, i.e. muscle type (110 kDa) and erythrocyte type (70 kDa). Muscle type calpastatin contains four internally repetitive sequences (Domains 1-4) and one nonhomologous sequence on the amino-terminal side (Domain L), whereas erythrocyte type lacks Domains L and 1. By immuno-blot analysis, chicken erythrocytes, nucleated cells, were found to contain muscle type calpastatin. In avian erythrocytes, diminution of the calpastatin molecule as in mammalian erythrocytes was not observed.

Animals↗
PubMed1991

Relationship between insertion torque and removal torque analyzed in fresh temporal bone.

Insertion torque and removal torque of fixtures were measured using human cadaver temporal bone and human living bone. The torque level for fracturing the threads of the bone was also measured. The maximum insertion torque exerted without causing a break in bone threads was 70.0 Ncm in bicortical bone and 50.0 Ncm in unicortical bone. The relationship between insertion torque and removal torque indicated a positive correlation between both sites. However, removal torque was lower than insertion torque in all measurements. The fracture points of bone threads were 77.0 Ncm in bicortical bone and 57.7 Ncm in unicortical bone by mean. Microfractures were observed mainly in the thread bases and were directed upward in the histologic specimens examined.

Aged↗
PubMed1991

[Serum ANP and ADH after subarachnoid hemorrhage and hyponatremia].

We determined serum atrial natriuretic peptide (ANP) and anti-diuretic hormone (ADH) on a time course basis in cases of subarachnoid hemorrhage and studied their influence on the development of hyponatremia. Twenty six cases of subarachnoid hemorrhage were admitted to our hospital in the past 1 year, and by the site of ruptured aneurysms, there were Acom 6 cases, ICA 6 cases, MCA 5 cases and VA BA 4 cases. Serum ANP and ADH levels were determined in the acute phase on Day 1-4, in the hyponatremia phase on Day 5-14 and in the chronic phase on Day 15 downward. Levels of not more than 130 mEq/l were regarded as hyponatremia. Cases showing other evident causes such as heart failure and renal insufficiency were excluded. In the normal control group (n = 20) which was admitted to this hospital for a close check-up, serum ANP was 26.5 +/- 11.6 pg/ml (10-50); levels of more than 50 pg/ml were regarded as being abnormally high. 1) Hyponatremia was observed in 7 cases (26-9%); the day of onset was 11.9 hospital day. The duration was 5.0 days and the minimum serum Na level was 126.4 mEq/l. 2) The serum ADH level was high regardless of whether or not there was the development of hyponatremia in the acute phase but tended to decrease gradually and became normal in the hyponatremia phase. 3) The serum ANP level in the cases of hyponatremia was 40.7 +/- 9.1 pg/ml in the acute phase, 69.0 +/- 25.7 pg/ml in the hyponatremia phase and 40.2 +/- 21.5 pg/ml in the chronic phase.(ABSTRACT TRUNCATED AT 250 WORDS)

Atrial Natriuretic Factor↗
PubMed1991

[Clinical investigation of 9 cases of urachal carcinoma].

Recently, we encountered 9 cases of urachal tumor and conducted clinical studies on its diagnosis and treatment. The chief complaint was gross hematuria in all cases. Computed tomographic scan was performed in 7 cases, and in all cases it revealed a tumor between the dome of the bladder and the Retzius' cavity. This finding strongly suggested a urachal tumor before pathological study. Magnetic imaging resonance (MIR) was performed in one of the 9 cases, and it indicated the extent of the tumor more clearly than computed tomography. Partial cystectomy + en bloc removal of the umbilicus and urachus was performed in 5 cases, radical cystectomy + en bloc removal of the umbilicus and urachus was performed in 2 cases, and radical cystectomy was performed in 2 cases. As postoperative therapy, radiotherapy was administered to 4 cases, and chemotherapy was administered to 4 cases. One case died from cancer after 4 years and 10 months, while the other 8 cases are being treated on an outpatient basis without any local recurrence. All 5 patients who underwent partial cystectomy are alive (mean survival time: 97 months). Thus we believe that cure can be achieved by partial cystectomy and adjuvant therapy. In addition, serum carcinoembryonic antigen is thought to be useful as an indicator of residual tumor or local recurrence.

Adenocarcinoma↗
PubMed1991

[Internal carotid artery stenosis and external carotid artery occlusion with unusual extracranial collateral pathway: case report].

A patient with stenosis of the internal carotid artery and occlusion of the external carotid artery associated with an unusual extracranial collateral pathway is presented. A 63-year-old man was hospitalized for sudden onset of black-out after urination. He was alert, and no neurological deficit was found. MRI showed multiple lacunae in the bilateral putamens. Cerebral angiogram demonstrated severe stenosis of the left internal carotid artery at its cervical segment and complete occlusion of the left external carotid artery at its origin. The ascending pharyngeal artery originated from the left internal carotid artery above its stenotic lesion and the superior thyroid artery originated from the left common carotid artery. The sternocleidomastoid branch from the left superior thyroid artery and the muscular branch from the left vertebral artery anastomosed with the muscular branch of the ascending pharyngeal artery. The ascending pharyngeal artery maintained patency of the internal carotid artery. It is important to perform vertebral angiography when there is proximal occlusion and severe stenosis of the internal carotid artery, and when the ascending pharyngeal artery has not been clearly identified as a branch from the ipsilateral external carotid artery on the common carotid angiogram.

Arterial Occlusive Diseases↗
PubMed1991

[Effects of extracellular K+ concentration on the ERG c-wave].

The effects of K+ concentration in the perfusate on ERG were examined in bullfrog neural retina-retinal pigment epithelium-choroid preparations. In the range of K+ concentration between 1.0 and 6.0 mM, an increase in K+ concentration caused a decline in the b-wave amplitude, while leaving the b-wave peak latency almost unchanged. An increase in K+ concentration in the range between 1.0 and 3.0 mM enhanced the c-wave amplitude, while an increase in K+ concentration in the range between 4.0 and 6.0 mM diminished it. An increase in K+ concentration tended to shorten the time course of the c-wave. These results suggest that variations in the K+ concentration in tissue fluid may partially account for the marked inter- and intra-individual variations of the c-wave.

Animals↗
PubMed1991

The early phenomena of restenosis following percutaneous transluminal coronary angioplasty.

In man the early onset of fibrocellular tissue reaction, which leads to restenosis following an initial successful percutaneous transluminal coronary angioplasty, has been poorly documented because of limited opportunities to study this phenomenon. The present study focused on early changes in seven patients who died within 20 days of a percutaneous transluminal coronary angioplasty procedure. Differences in the mode of laceration were noted between concentric and eccentric plaques. In the former, tears occur at the thinnest site of the plaque or within an already attenuated fibrous cap. The injury usually remains limited to the plaque. In eccentric lesions lacerations tend to occur in the non-atherosclerotic wall segment, affecting the musculoelastic layers and the media. A further site of predilection is the border zone between the non-affected wall and the plaque. Early tissue response is characterized by fibrin-platelet depositions, and a proliferation of macrophages and spindle-shaped cells. The latter express the immunocytochemical characteristics of smooth muscle cells. However, differences occur according to the type of tissue injured. Laceration of the musculoelastic layers or media causes a rapid and extensive cellular response. Injury to an atheroma, on the other hand, mainly causes plaque fissures with either mural thrombosis and total luminal obstruction, extensive plaque haemorrhage or a 'wash-out' of the atheromatous debris. In the latter case the lining of the 'crater' shows an accumulation of macrophages and, once more, a proliferation of spindle-shaped cells. The present observations support the concept that the early response is due to an interaction of platelet-fibrin thrombus and smooth muscle cells, with a possible role for macrophages.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1991

[In vitro studies on the expression of c-myc oncogene product in gynecological cultured cancer cells].

In this study, immunocytochemical and biochemical detection of c-myc protein in gynecological cultured cancer cells were performed together with gene expression of the cells. OMC-1 (cervical squamous carcinoma cell line), OMC-2 (endometrial adenocarcinoma cell line), OMC-3 (ovarian mucinous adenocarcinoma cell line) and OMC-4 (cervical adenocarcinoma cell line) were used. Immunocytochemically, c-myc protein was detected in both nuclei and cytoplasms of cultured cells when they were fixed in 95% ethanol, 10% formalin and 4% paraformaldehyde (PFA) including 10mM NaCl. However, it was detected in the nuclei of almost all of the cells in nuclei of the cells when they were fixed in 4% PFA including 1,000mM NaCl. Western blotting against a nuclear fraction of the cells demonstrated 66Kd protein in OMC-1 and 62Kd protein in OMC-2,3,4, respectively. They were completely absorbed by c-myc synthetic peptide. However, there was no reaction against the cytoplasmic fraction of the cells. Slot blot hybridization against the DNA of the cells demonstrated 15 times and 5 times c-myc gene amplification in OMC-2 and OMC-4, respectively. These results suggest that OMC-1,2,3,4 can be used as positive controls for the immunocyto- and histochemical detection of c-myc protein in clinical materials. However, it must be noted that the redistribution of c-myc nuclear protein into the cytoplasm may occur in the fixation process.

Autoradiography↗
PubMed1991

[A successful surgical treatment of impending rupture of the thoracoabdominal aneurysm in the aged].

In a case with impending rupture of the thoracoabdominal aneurysm of a 78-year-old aged who was admitted to our hospital as chief complaints of dysphagia, sense of pressure in the thoracic region and bloody sputum, resection of the aneurysm and patch graft aortoplasty were carried out with the aid of partial cardiopulmonary bypass. The maximum diameter of the aneurysm was 12 cm, and adhered partially with lung, and a very thin ejected region of the wall was noted. Enlargement of the saccular aneurysm in the false lumen of the dissecting aortic aneurysm accompanying with massive mural thrombus was noted. The patch graft aortoplasty was performed because back bleeding from four sets of intercostal arteries (Th 9-Th 12) was remarkably noted. The postoperative course was uncomplicated, and dysphagia was disappeared without paraplegia. This case is considered to be the oldest one who was undergone the the thoracoabdominal aneurysm in our country.

Aged↗
PubMed1991

[Cancer metastasis and recurrence from the standpoint of amplification and expression of oncogenes in human malignant tumors].

We investigated the amplification and expression of oncogenes in human malignant tumors. The survival rates of patients with c-erbB, c-erbB-2, and int-2 oncogene amplification/expression were significantly lower than those of the patients without gene amplification/expression. Many distant organ metastases were observed in esophageal cancer patients with int-2 amplification, in gastric cancer patients with c-erbB-2 over-expression, and in breast cancer patients with int-2 and c-erbB-2 amplification. These results suggest that the amplification/expression of these oncogenes may serve as good markers for determining the biological malignancy of cancers.

Gene Amplification↗