Biomedical subjects
Publications and source records attributed to M Uchida.
The present study reports the characteristic performance using lung segmental phantom filled in Tc-99m-pertechnetate. To evaluate the segmental defect in lung perfusion scintigraphy, we applied Bull's eye analysis in addition to planar image set. Bull's eye analysis especially facilitated the interpretation in both middle and lower lobes.
Noninvasive therapy for urolithiasis such as percutaneous nephroureterolithotomy (PNL) has progressed rapidly during the last few years. Several kinds of non-mechanical disintegration methods such as electrohydraulic lithotripsy, ultrasound lithotripsy, stone disintegration by laser irradiation, electric drill disintegration, microexplosion lithotripsy and extracorporeal shock wave lithotripsy have contributed to this advancement. However, these methods are not without some problems, such as effect and technique. This paper describes the theory and effectiveness of these non-mechanical methods except extracorporeal shock wave lithotripsy, especially of microexplosion lithotripsy in detail.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
The present study was designed to determine whether the long axis of the left ventricle is elongated in patients with aortic regurgitation. Among 445 patients with valvular disease who were followed in our hospital from April 1986 to February 1987, 14 with aortic regurgitation [AR: age: 46.1 (mean) +/- 17.6 (standard deviation) years] and 17 with mitral regurgitation (MR: age: 48.8 +/- 18.0 years) were selected for analysis. They all had optimal quality images in the apical view of the two-dimensional echocardiograms adequate for the evaluation and moderate to severe regurgitation at the time of Doppler examination. The control group consisted of 15 subjects without evidence of organic heart disease (age: 44.9 +/- 17.7 years). There was no difference in the mean duration of the clinical course between AR (14.9 years) and MR (13.4 years). The following measurements were made in the apical right anterior oblique view: Lo (long-axis distance of the outflow tract); from the left ventricular apex to the center of the aortic annulus, L (long-axis distance of the left ventricle); from the apex to the junctional point between the aortic and mitral valves, Li (long-axis distance of the inflow tract); from the apex to the center of the mitral valve ring, and d1, d2 and d3 (apical, middle and basal short axes of the left ventricle); the distances perpendicular to the each long axis at the levels of 1/4, 2/4 and 3/4 of the long axis. All data were corrected by means of the calibration scale, and compared as indices divided by the body surface area.(ABSTRACT TRUNCATED AT 250 WORDS)
Explore the source record for details and available documents.
The relation between oral leukoplakia and its malignant progression was clinicopathologically analysed by using the long-term follow-up cases treated in Cancer Institute Hospital, Tokyo, for 37 years (1950-1987). A total of 89 oral leukoplakia cases had been followed up for 11 years and 3 months on average, and the diagnosis of each case was histologically confirmed on biopsy. This series consisted of 56 male and 33 female patients. During the follow-up period, malignancy was found in 8 cases, at 5 years and 9 months on average after the diagnosis of leukoplakia. Among the cases being followed over 10 years, an incidence of malignant progression of benign leukoplakia was estimated as 10.25%. According to the size of leukoplakia, the lesions measuring over 1 cm in diameter showed higher risk for cancer development than the smaller ones. In the follow-up of leukoplakia, dysplasia was identified in 6 cases, and all of them were mild in degree. Moreover, cancer developed in 2 out of these 6 dysplasia cases.
Circulatory and respiratory changes following a mechanical compression of the brainstem of the rabbit were studied. Under light ether anesthesia, the animal was tracheostomized, immobilized by pancuronium bromide, and mechanically ventilated, following which the skull was fixed and suboccipital craniotomy was performed, and finally the brainstem was exposed. The site of compression was selected as (A): 3mm lateral, 3mm rostral, (B): 2mm lateral and (C): 3mm lateral, 3mm caudal, of the obex, respectively. After inhalation of ether was discontinued and elimination of the effect of muscle relaxant was confirmed, the direct compression with glass stick of 0.8mm in diameter was carried out. In each point, vertical compression of 1mm, 2mm and 3mm, was performed stereotaxically. In each depth of the compression, 3 to 4 animals were studied. The blood pressure was measured directly in the femoral artery and the respiratory changes by impedance pneumography. The circulatory changes, biphasic change of arterial pressure and/or transient bradycardia occurred earlier than the respiratory changes in 2mm and 3mm depth of the compression in all 3 points. CO2 response curve with rebreathing method was obtained before and 15 minutes after the compression to evaluate the degree of damage of the medullary respiratory center. The depression of CO2 response curve was observed only in 3mm compression depth at the point (A). It is concluded from this study that excessive invasion of the brainstem during the suboccipital craniotomy under controlled ventilation could be detected through the observation of the changes in circulatory parameters.
Explore the source record for details and available documents.
The two types of colonial variants of Mycobacterium avium complex, SmT (smooth, transparent, irregular) and SmD (smooth, opaque, dome-shaped) variants, were examined for their triggering activity for macrophage (M phi) respiratory burst, based on chemiluminescence (CL). SmD variants elicited an intense CL from zymosan A-induced M phi s in a dose-dependent manner, although SmT variants induced much lower M phi CL. The M phi s could steadily phagocytose SmT variants, although the phagocytizing rate was considerably lower compared to the case of SmD variants. Treatments of SmD variants with Tween 80, pronase and some endoglycosidases such as alpha-amylase, cellulase, dextranase and pectinase, heating (100 degrees C, 15 min), and delipidation by CHCl3-methanol extraction resulted in a marked reduction in the M phi CL-triggering activity of the SmD variants. Thus, the M phi CL-triggering ligand(s) seems to possess glycolipoprotein-like moieties. Tween 80-treatment of SmT variants, which is known to deprive the polysaccharide outer layer specific to the colonial variants, failed to recover the M phi CL-triggering activity of SmT variants. Therefore, the remarkably reduced M phi CL-triggering ability of the SmT variants may be caused by the extremely lowered expression of the M phi CL-triggering ligands rather than by masking of the CL-triggering ligands by SmT-specific outer layer.
Explore the source record for details and available documents.
We isolated a mouse monoclonal antibody that disrupts Ca2+-dependent cell-cell adhesion of amphibian (Xenopus laevis) cells. When added to culture medium, the monoclonal antibody completely disrupted cell-cell adhesion of amphibian cells in monolayer culture and specifically inhibited Ca2+-dependent cell-cell adhesion of dissociated cells in reaggregation experiments. The monoclonal antibody recognized a 140 kDa cell surface glycoprotein antigenically different from the previously reported Ca2+-dependent cell-cell adhesion molecules (cadherins).
We treated 105 consecutive patients with bladder calculi by microexplosion lithotripsy, with a success rate of 100 per cent. The weight of the removed calculi averaged 18 gm., with a range of 2 to 305 gm. For the procedure spinal anesthesia was used in 78 patients, while general anesthesia was used in 24. Minor extraperitoneal bladder perforation occurred only once and it resolved spontaneously several days later. Presently, we consider microexplosion lithotripsy to be the treatment of first choice for bladder calculi.
A lymph node was examined from a 4-year-old female child with documented "Mo1" (CR3) deficiency. There was hypoplasia of the lymph node, with small, poorly delineated germinal centers and overall lymphopenia. Retrospective analysis of the clinical course of a decreased elder sister of the index patient indicated that the elder sister probably also had "Mo1" deficiency. Review of findings at autopsy revealed severe hypoplasia of all lymphoid organs with lymphopenia. Our observations suggest that lymphoid tissue hypoplasia may be a feature of CR3 deficiency and is likely a result of the defective lymphocyte adherence functions to endothelium associated with absent lymphocyte function-associated antigen (LFA-1).
Ascorbate oxidase from pumpkin (Cucurbita sp.) was purified from a commercially available preparation. A single polypeptide band with M(r) 64,000 was detected after sodium dodecylsulfate-polyacrylamide gel electrophoresis of the purified enzyme. In double immunodiffusion tests, antiserum against the purified preparation formed a single precipitin line with the crude extract from pumpkin fruit tissue or the callus as well as with the purified preparation. Immunological blotting method showed that mol wt of ascorbate oxidase subunit in pumpkin callus was the same as that of the purified preparation. Analysis with the single radial immunodiffusion method showed that the increase in ascorbate oxidase activity during the growth of pumpkin callus correlated with an increase in the enzyme protein. Furthermore, enzyme protein in the callus grown in the presence of 10 micromolar CuSO(4) for 2 weeks was about eight times that grown in the presence of 0.1 micromolar CuSO(4). The synthesis of ascorbate oxidase in pumpkin callus may be induced by copper, a prosthetic metal of the enzyme, or copper may help stabilize the enzyme against proteolytic breakdown.
Explore the source record for details and available documents.
Radiotherapy is more commonly employed in Western countries than in Japan. This therapy is highly effective according to a site and kind of cancer, and is superior to surgical treatment, not in a few, as for functional conservation, safety and radicalness. Reduced operation for the treatment of cancer occurring in the head or neck, ophthalmological tumor and skin cancer is herein discussed from a standpoint of the combination mainly with radiotherapy.