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Biomedical subjects

M Tsutsui

Publications and source records attributed to M Tsutsui.

At least 127 records · Page 7Linked to original sources
PubMed1984

Calcium-activated, phospholipid-dependent protein kinase in pig epidermis.

We investigated calcium-activated, phospholipid-dependent protein kinase in pig epidermis. Pig epidermal homogenates were centrifuged at 30,000 g for 30 min, and the supernatant was applied on a DEAE-cellulose column for purification. The partially purified enzyme was stimulated by simultaneous addition of Ca2+ and phospholipid. Successive addition of small amounts of diolein further activated the enzyme activity. The calcium-activated phospholipid-dependent protein kinase preferentially phosphorylated serine residues and its endogenous substrate protein in the pig epidermis has a molecular weight of about 97,000.

Animals↗
PubMed1984

Ultrastructural localization of acid phosphatase activity in plasma cells containing Russell's bodies in periodontitis.

Acid phosphatase activity was examined ultracytochemically in gingival specimens to elucidate the response of plasma cells to Russell's bodies. The acid phosphatase activity was discernible in lysosomes of various morphology, some of which contained Russell's bodies. The acid phosphatase activity was stronger in the peripheries of such lysosomes, but weak activity was also found inside Russell's bodies. These findings indicated that at least some of Russell's bodies formed within the plasma cells were degraded in autophagolysosomes.

Acid Phosphatase↗
PubMed1983

Analyses of risk factors of ischemic heart disease in diabetics--multivariate analyses.

In order to confirm the principal risk factor of ischemic heart disease (IHD) in diabetes, multivariate analyses were performed. ST depression in electrocardiogram (ST-ECG) and 18 other clinical-laboratory findings (sex, age, duration of diabetes, blood pressure, cholesterol, HDL-cholesterol, triglyceride, etc.) were measured in 70 non-insulin dependent diabetes mellitus patients. ST-ECG findings were divided into five ranges as an index of the severity of IHD, based on the assumption that the degree of ST-ECG would provide a reasonable correlation to the grade of IHD. In partial correlation analysis, the degree of ST-ECG was significantly correlated both to the level of triglyceride (r = 0.455, p less than 0.001) and to blood pressure (r = 0.392, p less than 0.01), but not to the other 16 variables. Three selected variables (blood pressure, triglyceride and atherogenic index) were sufficient to provide a satisfactory discrimination between patients with and without IHD. Five selected variables (sex, blood pressure, triglyceride, atherogenic index and weight index) were sufficient for evaluation of regression. These results suggest that the high level of triglyceride and hypertension are essential risk factors of IHD in diabetes. It is noticeable that the high level of triglyceride is one of the independent risk factors of IHD in diabetes; it does not depend on the degree of control of hyperglycemia or on the other variables.

Adult↗
PubMed1982

[Analgesic and narcotic antagonist effects of buprenorphine (author's transl)].

Pharmacological properties of buprenorphine were studied in comparison with those of morphine and pentazocine. Buprenorphine was more potent than morphine and pentazocine in analgesic tests, using chemical, thermal, pressure, and electrical stimulation as the nociceptive stimuli. Buprenorphine exhibited analgesic activity in the D' Amour-Smith's test at high stimulus intensity and in the Haffner's test, while pentazocine exhibited little or no analgesic action in these tests. Buprenorphine showed a bell-shaped dose-response curve in the mouse D'Amour-Smith's test at high stimulus intensity. Analgesic action of buprenorphine was antagonized by naloxone administered before buprenorphine, but not antagonized by naloxone administered after buprenorphine. Duration of the analgesic action of buprenorphine was longer than those of morphine and pentazocine. Buprenorphine decreased the amplitudes of evoked potentials which were recorded from the nucleus ventralis posteromedialis, nucleus centralis lateralis, and periaquaductal central gray. As a naloxone antagonist, Buprenorphine was equivalent or less potent than naloxone and more potent than pentazocine. Tolerance developed to the analgesic activity of buprenorphine, but development of tolerance to buprenorphine was less than that of tolerance to morphine. It was concluded that buprenorphine may be a useful analgesic drug because of its high intrinsic activity and long duration of action.

Analgesics↗
PubMed1980

Dentinal response against carious invasion: localization of antibodies in odontoblastic body and process.

The pulpal origin of dentinal immunoglobulins was demonstrated by means of immunohistological methods. Immunoglobulins were located both in the cytoplasm of odontoblasts in pulp and at odontoblastic processes in dentin. Positive reactivity of the immunoglobulins to antigens was confirmed using peroxidase-immunized rabbits. IgG, IgA, IgM, C3, and C4 were observed on some invasive bacteria in human carious dentin.

Animals↗
PubMed1980

Phototoxicity of the chemotherapeutic agents hematoporphyrin D, meso-tetra(p-sulfophenyl)porphine and zinc-tetra(p-sulfophenyl)porphine.

Interest in natural and synthetic porphyrins as tumor-localizing agents, tumor-photoinactivating agents and anti-trypanosomal drugs prompted a laboratory evaluation of the phototoxic potency of these compounds. At UV wavelengths greater than 3200A three porphyrin compounds were significantly more phototoxic than the positive control, 6,8-dichloro-2-phenyl-alpha-2-piperidyl-4-quinoline methanol. Phototoxicity was seen after intraperitoneal administration but not after oral administration. Minimum effective phototoxic doses of Hematoporphyrin D were < 2.40 mg; TPPS < 1.25 mg/kg, and ZnTPPS < 1.24 mg/kg. Hematoporphyrin D produced toxic deaths at doses of 37.5-150 mg/kg.

Animals↗
PubMed1979

[Pharmacological properties of procaterol, a newly synthetized, specific beta 2-adrenoceptor stimulant. Part I. Effects on the CNS (author's transl)].

Effects of procaterol (PRO) on the CNS were investigated in comparison with those of salbutamol (SAL) and isoproterenol (ISO). PRO, 15 to 50 mg/kg given subcutaneously suppressed spontaneous movement in mice, rats and rabbits and with a large dose, 1000 mg/kg, the animals became quiet and immobile. In dogs, PRO produced similar symptoms and in addition, there was nausea and vomiting. The animals recovered within 3--8 hours. ID50's in depressing spontaneous movement were 20.2 and 245 mg/kg for PRO, 51.1 and 133 mg/kg for SAL and 2.37 and 143 mg/kg for ISO, respectively, both by the subcutaneous and oral routes of administration. Methamphetamine induced increase in motility and fighting behavior was also suppressed by PRO when similar doses were given. PRO had no effect on coordinating movement, halothane anesthesia, drug and electric stimulation induced convulsions and body temperature, and there was no muscle relaxant action. However, PRO in large doses prolonged sleeping time with hexobarbital. The analgesic effect of PRO was not observed with Haffner's and Landall Selitto's methods but acetic acid induced writhing was suppressed by PRO. PRO had little effect on spontaneous EEGs either cortical or from deep structures, and EEG arousal responses. The effects of PRO on the CNS were slight and nonspecific, and similar to those of SAL and ISO.

Adrenergic beta-Agonists↗