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Biomedical subjects

M Tsuji

Publications and source records attributed to M Tsuji.

At least 523 records · Page 29Linked to original sources
PubMed1981

Isolation of antigenically active components from leptospiral serovar-specific lipopolysaccharide antigen by alkaline treatment.

The serovar-specific main antigen (TM antigen) of Leptospira interrogans serovar canicola, which as lipopolysaccharide properties, was treated with 0.1 N sodium hydroxide. This treatment degraded the antigen into two major antigenic components, one of high and one of low molecular weight. The component with the lower molecular weight (approximately 4,000 daltons) consisted mainly of carbohydrates, having lost almost all of the fatty acid and protein components of the original antigen. Although the substance lacked immunoprecipitable activity, it continued to show serovar-specific inhibitory potency in a radioimmunoassay system as well as in a microscopic immunoagglutination reaction of the organisms. The antigenic activity of the compound was also reduced by periodate oxidation as was that of the TM antigen. A component with the same chemical and physicochemical properties was also produced by alkaline treatment from a different serotype TM antigen (serovar kremastos Kyoto), but it showed no antigenic activity.

Bacterial Proteins↗
PubMed1981

Developmental alteration in RNA metabolism of E1 mouse brain.

The contents of brain RNA rose rapidly until 14 days after birth and then dropped. They were lower in the E1 than in the non-convulsive mice between 56 and 100 days of life. The contents of acid-soluble UMP and their specific radioactivities, three hours after an intracranial injection of [3 H]-orotic acid, were not different in the same age of E1 and non-convulsive mouse brains. From four to 20 days after birth, the specific and relative specific radioactivities, representing the ratio of brain RNA synthesis, of the E1 mice were lower than those of the non-convulsive strains, while the E1 mice, applying convulsions once a week, exhibited higher values during the periods from 56 to 100 days of age as compared with the non-stimulated E1 or non-convulsive strains.

Aging↗
PubMed1981

Uterine teratoma with lymphoid hyperplasia.

A mature teratoma of the uterine cervix, which was found nine months after delivery, was presented. This case was the ninth in the world, the first in Japan. Differential diagnosis and histogenesis of the uterine teratoma were discussed on the basis of histologic findings of this tumor and a review of literatures. Among the microscopic findings obtained, the conspicuous lymphoid tissue in the tumor was worthy of note, because it could be speculated to be an immunological reaction to the cells composing the tumor. It was also suggested from the post-partum occurrence that a fetal-cell origin would be possible in the histogenesis, although further positive studies on this opinion should be necessary in coming cases.

Adult↗
PubMed1980

The common identity of UDP-N-acetylgalactosamine 4-sulfatase, nitrocatechol sulfatase (arylsulfatase), and chondroitin 4-sulfatase.

Evidence is presented indicating that three sulfatase activities towards UDP-N-acetylgalactosamine 4-sulfate, nitrocatechol sulfate, and chondroitin 4-sulfate are functions of the same hen oviduct enzyme. Using chondroitin [35S]sulfate from chick embryo cartilage, it is shown that hydrolysis of ester sulfate by this enzyme is limited to 4-sulfate groups occurring in the non-reducing terminal N-acetylgalactosamine 4-sulfate and N-acetylgalactosamine 4,6-bissulfate residues.

Animals↗
PubMed1980

Phenyl phosphate as a water-soluble analog of dolichyl phosphate in microsomal mannosyltransferase systems.

Incubation of rat liver microsomes with GDP-[14C]mannose and phenyl phosphate for 30 min, followed by paper-chromatographic analysis, revealed the presence of a new radioactive compound that was isolated and characterized as phenyl beta-D-mannosyl phosphate. The enzyme catalyzing this reaction was very closely related to a dolichyl beta-D-mannosyl phosphate-synthesizing activity found in the same microsomes. The two activities were affected in a parallel fashion by mild heating and were found in the same proportions in whole microsomes, in rough-surfaced microsomes, and in smooth-surfaced mirosomes. Increasing the concentration of phenyl phosphate from 0 to 15 mM resulted in a progressive reduction in the rate of mannose incorporation into endogenous dolichyl phosphate. It is concluded that the two reactions are catalyzed by a single enzyme. The labeled product from phenyl phosphate was rapidly excluded from the microsomes, while the product from dolichyl phosphate was retained in the microsomes, suggesting that phenyl phosphate may act as a mannose-trapping agent, thereby inhibiting mannosyltransferase reactions involving dolichyl phosphate as a glycosyl carrier.

Animals↗
PubMed1980

The effect of oestrogens on human calcitonin secretion after calcium infusion in elderly female subjects.

The effect of oestrogen administration (4-6 weeks) on the response of human calcitonin (hCT) secretion to 5 min calcium infusions was studied in ten elderly women. There was no significant difference in mean basal plasma hCT levels before and after oestrogen administration. However, the mean increment in plasma hCT in response to calcium infusion (delta hCT) increased significantly (P < 0.001) from 21.9 +/- 6.6 (mean +/- SE) before treatment, to 79.6 +/- 15.5 ng/l after oestrogen administration. Mean serum calcium levels decreased significantly (P < 0.001) from 2.42 +/- 0.06 before to 2.19 +/- 0.07 mmol/l after oestrogen treatment. Mean plasma immunoreactive PTH (iPTH) levels increased significantly (P < 0.05) from 521 +/- 41 before to 696 +/- 96 ng/l after oestrogen treatment. To exclude out the possibility that the decreased serum calcium level itself might have influenced delta hCT, 1 alpha-hydroxycholecalciferol (1 alpha-OH-D3) was administered with oestrogens. While this resulted in a slight increase in serum calcium level, there was no significant difference in delta hCT in response to calcium infusion following oestrogen treatment alone, and after combination therapy of oestrogen and 1 alpha-OH-D3. The primary action of administered oestrogen may be in stimulating hCT secretion which results in a decrease in plasma calcium concentration and an increase in plasma iPTH level.

Adolescent↗
PubMed1980

Mesothelioma with lung cancer complicating asbestosis.

The patient had been employed in an asbestos factory for four years from the age of 16. Five years ago, she complained of sputum and cough, and she was treated for chronic bronchitis. From March, 1977, when she was 53 years old, hydrothorax and ascites increased, her weight decreased and she was hospitalized for cachexia. The clinical diagnosis of malignant diffuse mesothelioma was made on the presence of atypical cells in the effusions. Atypical cells showed a positive colloidal iron staining test and positive hyaluronidase digestion test. Asbestos bodies were found in the sputum. The patient died on February 1978. Postmortem confirmed asbestosia and mesothelioma which was scattered over the pleura, pericardial sac, diaphragma, peritoneum and pancreas. In addition, bronchiolo-alveolar cell type lung cancer was found localized in the lower lobe of the left lung. The electron beam diffraction disclosed the asbestos as amosite (brown asbestos).

Asbestosis↗
PubMed1980

Visceral larva migrans caused by Trichuris vulpis.

Two brothers with visceral larva migrans caused by Trichuris vulpis were diagnosed after they had been investigated for an eosinophilia. Both patients were almost asymptomatic. The diagnosis of visceral larva migrans was based on the results of immunoelectrophoretic studies and no liver biopsy was performed. After administration of thiabendazole, the number of eosinophils and serum total IgE levels gradually decreased, and the patients have remained well.

Animals↗