Biomedical subjects

M Trope

Publications and source records attributed to M Trope.

At least 91 records · Page 5Linked to original sources

PubMed1991

Dye leakage in retrofilled dog teeth and its correlation with radiographic healing.

In a previous study in beagle dogs, statistically significant differences in healing were observed radiographically 6 months after apicoectomy and retrofilling with amalgam and varnish, glass ionomer cement, or a composite resin. The purpose of this study was to compare the leakage of these retrofilling materials and to correlate leakage data with healing. After the dogs were sacrificed, 48 roots were extracted. Half of the roots had been irradiated with a CO2 laser on the bevelled root surface after retrofilling. The roots were coated with nail polish, except for their bevelled surface, and were suspended together with positive and negative controls in 2% methylene blue for 8 h. After the specimens were longitudinally split, they were ranked according to the severity of dye penetration, as observed with a dissecting microscope. The differences in dye leakage among the three retrofilling materials were not statistically significant. Consequently, dye leakage did not correlate with the previously assessed healing. Dye penetrated into the root through the bevelled root surface, regardless of CO2 laser exposure of that surface.

Animals↗

PubMed1991

Flare-up rate of single-visit endodontics.

The purpose of the study was to compare the flare-up rate for single-visit endodontics among teeth without radiographic or clinical signs of apical periodontitis, those with radiographic or clinical signs of apical periodontitis not previously root-treated, and those with apical periodontitis where retreatment was performed. All teeth were instrumented to a predetermined minimum size with a 0.5 per cent solution of sodium hypochlorite being used as the irrigant. The root canal was obturated without regard to the presence or absence of symptoms or diagnosis of the apical condition. The patients were given written post-operative instructions and a prescription for 600 mg ibuprofen to be taken if mild to moderate pain developed. If severe pain and/or swelling developed, the patient was instructed to telephone immediately and was considered to have had a flare-up. Teeth without signs of apical periodontitis did not have any flare-ups. One flare-up occurred in 69 teeth with signs of apical periodontitis not previously root-treated. The majority of the flare-ups (3 of 22 teeth) occurred in teeth with signs of apical periodontitis requiring retreatment.

Acute Disease↗

PubMed1991

Vitality of human lip fibroblasts in milk, Hanks balanced salt solution and Viaspan storage media.

The purpose of the study was to compare the effect of storage in milk, Hanks balanced salt solution (HBSS) and Viaspan on the vitality of human lip fibroblasts. Confluent monolayers of the fibroblasts were grown in petri dishes. The growth medium was poured off and replaced in 27 dishes each with Viaspan, fresh whole milk and Hanks balanced salt solution respectively. Three of the original plates were analyzed at time zero to obtain the average number of cells each plate contained at confluence. At times ranging from 2 to 168 hours the average number of vital cells remaining was measured using the trypan blue exclusion test. The groups stored in milk maintained a high percentage of vital cells for 6 hours (68.2%). At 12 hours milk's effectiveness had dropped to 43.4% vital cells and it was not effective at all at 48 hours (0.024% vital cells). H.B.S.S. was extremely effective for 24 hours with 71.3% vital cells remaining. At 48 hours, the percentage of vital cells dropped to 38.0% and by 120 hours no cells survived. Viaspan was the most effective storage medium at all observation periods and at 168 hours still had 37.6% vital cells present.

Adenosine↗

PubMed1991

Pulp capping of immature teeth with anatomic anomalies.

This case report describes the treatment of two immature teeth with anatomic anomalies which created plaque retentive areas and in one case occlusal disturbance. Periodontal and pulpal consequences were inevitable. Reshaping of the teeth was carried out which as expected led to exposure of the pulp in both instances. Pulp capping was then performed under aseptic conditions. The vitality of the teeth was maintained and continued root development was observed.

Adolescent↗

PubMed1990

Relationship of intracanal medicaments to endodontic flare-ups.

The purpose of the study was to compare the effect of three intracanal medicaments on the incidence of post-instrumentation flare-ups. All teeth were instrumented to a predetermined minimum size using a 0.5% solution of sodium hypochlorite as the irrigant. Formocresol, Ledermix, and calcium hydroxide were placed in strict sequence irrespective of the presence or absence of symptoms or radiographic signs of apical periodontitis. The patients were given written post-operative instructions and a prescription for 600 mg ibuprofen to be taken if mild to moderate pain developed. If severe pain and/or swelling developed the patient was instructed to call the office immediately and was considered to have had a flare-up. Twelve flare-ups occurred in teeth with radiographic signs of apical periodontitis; none in teeth without periapical radiolucencies. Six of the twelve flare-ups occurred in retreatment cases and the other six occurred in teeth without previous endodontic treatment. No significant difference was found in the flare-up rate among the three intracanal medicaments.

Acute Disease↗

PubMed1989

Detection of HIV in the dental pulp of a patient with AIDS.

Dental pulp tissue from a patient with acquired immune deficiency syndrome (AIDS) was examined to determine the presence of the human immunodeficiency virus (HIV). The results found a high concentration of proviral HIV DNA.

Acquired Immunodeficiency Syndrome↗

PubMed1989

In vivo sealing ability of calcium hydroxide-containing root canal sealers.

It was the purpose of this study to determine the sealing ability of 2 commercially available calcium hydroxide-containing root canal sealers, CRCS and Sealapex. One hundred sixty single-rooted human teeth were used. The clinical crowns were removed and the roots were cleaned mechanically and by immersion for 24 h in 5% sodium hypochlorite. The root canals were then instrumented to size 80 at the apical foramen and the roots were sterilized by gamma-radiation. Under aseptic conditions the root canals were filled with a gutta-percha master cone size 70 and Sealapex (48 roots), CRCS (48 roots), and a zinc oxide-eugenol type sealer, Roth 801 (50 roots). The coronal aspects of the root canals were then sealed with zinc oxide-eugenol cement. In order to bring the roots and root filling materials in contact with tissue and tissue fluids, the roots were implanted subcutaneously in rabbits for 90 days and 1 year. Leakage as demonstrated by penetration of India ink was evaluated using a stereomicroscope. The best results were obtained with CRCS. Significantly less leakage occurred with both calcium hydroxide-containing sealers than with the traditional zinc oxide-eugenol sealer.

Calcium Hydroxide↗

PubMed1989

Differentiation of radicular cyst and granulomas using computerized tomography.

The purpose of this investigation was to study periapical lesions by means of computerized tomography to ascertain if this noninvasive method could be of value in distinguishing between radicular cysts and granulomas. Periapical radiographs were taken of the teeth of 60 human cadavers. Periapical radiolucencies were seen in conjunction with 33 teeth. Based on the periapical radiographs, an oral radiologist (J.P.) attempted to select 4 granulomas and 4 cysts from the 33 radiolucencies. Computerized tomography was performed on the root tips and the periapical lesions of these 8 teeth. The roots and periapical lesions were then surgically removed and prepared histologically for microscopic examination. In the tomographs, 7 of the periapical lesions had a cloudy appearance with a density similar to each other and to the surrounding soft tissue. In the eighth lesion a homogeneous dark area with a distinctly lower density could be distinguished from surrounding cloudy areas. Histologically, the dark area was shown to be an epithelialized cyst cavity. The other 7 lesions were granulomas. Thus, a cyst could be differentiated from periapical granulomas by computerized tomography because of a marked difference in density between the content of the cyst cavity and granulomatous tissue.

Diagnosis, Differential↗

PubMed1988