Search PubMed⌕ Search

Biomedical subjects

M Torisu

Publications and source records attributed to M Torisu.

At least 91 records · Page 5Linked to original sources
PubMed1986

Immunotherapy of cancer patients with BCG: summary of ten years experience in Japan.

Two thousand and four patients with various types of late stage cancer were treated with living BCG immunotherapy from 1972 to 1982. Fourty-five out of 1,112 patients were able to survive more than 5 years after initiation of this immunotherapy. All of these patients were noncurative operation except for 2 cases of hepatoma who received no surgery. We report the immunological and clinical aspects of the long term survivors of this treatment.

BCG Vaccine↗
PubMed1984

Lymphocyte locomotion in experimental allergic thyroiditis.

Experimental allergic thyroiditis was produced in rats by immunization with homologous thyroglobulin (Tg). The mechanisms of lymphocyte accumulation of the thyroid lesion were analyzed by using this experimental model. The sensitized lymph node cells were cultured with Tg. After 72 hr, the cell-free supernatant was found to contain a chemotactic factor for sensitized lymphocytes. Lymph node lymphocytes from animals immunized 3 weeks previously were the best source of such sensitized lymphocytes while the cells at the latter stage could not produce the factor. The culture supernatant was applied to a Sephadex G-100 gel filtration column. The results indicate that the molecular weight of the factor is around 12,400. Among lymph node lymphocytes, nylon-wool column nonadherent lymphocytes were the most responsive; adherent lymphocytes were not responsive. Thyroglobulin itself did not chemoattract lymphocytes obtained from rats immunized with Tg. This phenomenon was in contrast to macrophage chemotaxis in the same model in which macrophage itself can move toward Tg. In any event, these results seem to indicate that a chemotactic lymphokine may be playing an important role in the pathogenesis of lymphocyte accumulation of the thyroid lesion.

Animals↗
PubMed1983

Acid protease activity in thyroid gland from patients with Graves' disease.

Although the activity of lysosomal protease in Graves' thyroid is considered to be increased, there has been no quantitative method to estimate the protease activity in the thyroid tissue due to the contamination of thyroglobulin (Tg) which varies in susceptibility to the protease. In the present study, the proteolytic activity (PA) of thyroid lysosomal protease preparation (P25) separated from Tg was assayed using 125I labeled rat Tg. More than 95% of 125I-Tg was hydrolyzed at pH 4.0 without deiodination, and the pattern of liberated iodoamino acids resembled that of pronase digest except for a higher T3/T4 ratio. Thirty-seven Graves' thyroids and 15 paranodular thyroid tissues were assayed. PA, the specific PA (SPA; calculated as PA per mg P25 protein) and PA per DNA content in Graves' thyroids were significantly higher than those in controls. There were good correlations among PA, SPA and PA per DNA content of the thyroid tissue. PA and SPA in the thyroid from 29 patients with Graves' disease treated with propylthiouracil or methimazole did not correlate with serum thyroid hormone level immediately before surgery. In 8 patients treated with KI, PA from 5 patients whose serum thyroid hormone levels had been normalized were significantly lower than those from 3 patients who were still thyrotoxic.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1983

New approach to management of malignant ascites with a streptococcal preparation, OK-432. II. Intraperitoneal inflammatory cell-mediated tumor cell destruction.

Twelve patients with malignant ascites caused by gastric cancer were treated with intraperitoneal injections of a streptococcal preparation, OK-432. All had resolution of the ascites after OK-432 treatment. Neutrophils, macrophages, and lymphocytes increased in number in ascitic fluid samples. Some of the OK-432-induced inflammatory cells were attached to tumor cells. The absolute number of tumor cells decreased as the number of infiltrating inflammatory cells increased. Infiltrating lymphocytes were mainly E rosette-forming cells. Infiltrating macrophages were in an activated state. The infiltrating neutrophils, lymphocytes, and macrophages could inhibit DNA synthesis of the patient's own tumor cells in the ascitic fluid after OK-432 injection, but not before the injection. These results indicate that OK-432-induced neutrophils, lymphocytes, probably T cells, and activated macrophages may play an important role in tumor cell destruction in ascites. Moreover, as the number of tumor cells decreased, the ascitic fluid protein levels decreased. Decrease of the ascitic fluid protein level may suppress further accumulation of ascitic fluid, and the low protein level in ascitic fluid is likely to facilitate the reabsorption of the fluid into the bloodstream.

Adenocarcinoma↗
PubMed1982

Neutrophil-mediated tumor cell destruction in cancer ascites.

Intraperitoneal injections of OK-432, originated from Group A streptococcus pyogens of human origin, were administered to 77 patients with ascites caused by cancer of the digestive tract. Complete disappearance of effusion was observed in 43 cases, its reduction in five out of 77. They received cytologic examination of ascites daily before and after OK-432 injection. One of these patients, a 77-year-old man with carcinomatous peritonitis due to gastric cancer, showed an interesting phenomenon after OK-432 injection. All adenocarcinoma cells in his ascites disappeared at least with 36 hours after OK-432 injection with increasing number of intraperitoneal neutrophils. In addition, neutrophils collected from his ascites or peripheral blood showed cytostatic effect on his ascites-derived tumor cells in vitro. His neutrophil-depleted intraperitoneal cells, however, had no significant effect on DNA synthesis of his tumor cells in vitro. OK-432 itself had no significant effect on DNA synthesis of his tumor cells in vitro. This report describes a patient in whom OK-432-induced neutrophils may play an important role in his tumor cell destruction in ascites.

Adenocarcinoma↗
PubMed1981

Chemotaxis of Kupffer cells isolated from rodent liver.

Kupffer cells (KC) were isolated from the liver of guinea pigs, rats, and mice using enzymatic digestion with collagenase, followed by differential centrifugation and plastic adherence. Purity of the isolated KC was 96.0 +/- 2.2, 97.2 +/- 2.1, and 96.0 +/- 2.3 per cent in guinea pigs, rats, and mice respectively. These isolated KC were tested for migratory response to bacterial factor, which is one of the representative chemotactic factors for inflammatory macrophages, using a modified Boyden chamber technique. KC from the three animal species similarly migrated to the bacterial factor. The migratory response of the KC to the bacterial factor is due to chemotaxis but not chemokinesis. These results show the possibility that KC may recognize a chemoattractant and directionally migrate to it.

Animals↗
PubMed1981

Serum inhibitory factor for guinea pig macrophage and neutrophil chemotaxis found in cancer patients.

The effect of sera from 107 patients with gastrointestinal cancer on macrophage chemotaxis was studied using a modified Boyden chamber technique. A macrophage chemotactic inhibitory factor (CIF) was found in sera of advanced cancer patients. The CIF activity was significantly stage-related, and increased especially in patients with negative cutaneous responsiveness in purified protein derivative, dinitrochlorobenzene and keyhole limpet hemocyanin skin tests, and with low circulating T-lymphocyte levels. In addition, the CIF was found to suppress neutrophil as well as macrophage chemotaxis. suppress neutrophil as well as macrophage chemotaxis. The mode of action and the characteristics of the CIF were investigated, and the following results were obtained. (1) The CIF directly interacted with macrophages and neutrophils and suppressed their chemotactic activities without apparent cytotoxic effect on these cells. (2) On Pevikon block electrophoresis, the CIF activity was most prominent in the alpha-globulin region, although considerable heterogeneity and broadness of the CIF bands were observed. (3) Dialysis and lyophilization had no effects on the CIF activity. On heating at 56 degree for 30 min, the CIF activity seemed to be decreased. This study indicates that CIF in cancer patients may be detrimental to the host immune defense against tumor growth by producing defects of macrophage and neutrophil chemotaxis in vivo.

Adenocarcinoma↗
PubMed1979

Role of thymectomy in the surgical treatment of myasthenia gravis.

Of the 26 patients with myasthenia gravis undergone thymectomy, 11 cases had either benign or malignant thymoma as judged not only by hitological examination but also by their clinical and operative findings. Age of initial onset ranged from 13 to 64 years old. Fifteen out of 26 (58 per cent) benefited from thymectomy. Duration of the symptom from the onset to the operation and the presence or absence of the thymoma are not related to their outcome. Benign or malignant nature of thymoma should not be determined by histological examination alone but by combined evaluation of clinical and operative findings. Serial studies of serum immunoglobulin levels before and after thymectomy suggested that this disorder could be associated with humoral antibody (IgG). HLA typing of the patients with myasthenia gravis did not indicate the presence of any specific antigens.

Adolescent↗
PubMed1979

Abnormal lipoprotein appearing in plasma of patients who received a ten percent soybean oil emulsion infusion.

In 42 of 43 surgical patients who received a 10% soybeam oil emulsion (Intralipid), abnormal lipoprotein was detected in their plasma 1 to 2 days after the initial Intralipid infusion. This abnormal lipoprotein was proven to appear as a result of the infusion of soybean oil emulsion regardless of the patient's original diseases, age, sex, liver function, or concomitantly administered solutions. In addition, this abnormal -ipoprotein was found to have various similarities to lipoprotein-X (LP-X) which is found in plasma from patients with obstructive jaundice or familial lecithin:cholesterol acyltransferase deficiency. Therefore, this abnormal lipoprotein was tentatively named LP-X--like substance (LP-X-LS). A comparison of the properties of LP-X and LP-X-LS was performed and the following results were obtained: (1) LP-X-LS migrated toward the cathode on Bacto-Agar gel electrophoresis similarly to LP-X; (2) the stability of LP-X and LP-X-LS against heating and freezing were almost equal under various conditions; (3) LP-X-LS could be absorbed by anti--LP-X serum; (4) LP-X-LS existed in low density fraction (d = 1.063) separated by ultracentrifugation from plasma; (5) electron microscopic study of low-density lipoprotein particles from LP-X-LS positive plasma revealed that LP-X-LS had a similar ultrastructure to LP-X. From these results it is suggested that LP-X-LS is an abnormal lipoprotein quite similar to LP-X.

Adult↗
PubMed1979

Studies on the immunopotentiating effects of a streptococcal preparation, OK-432. I. Enhancement of T cell-mediated immune responses of mice.

The effects of the anti-tumour agent OK-432 on the immune response to hamster erythrocytes (HRBC) and nucleated chicken erythrocytes (CRBC) were studied in inbred SL mice. Mice were treated repeatedly with OK-432 before immunization with erythrocytes in saline. The cytotoxicity of CRBC-primed spleen cells, as demonstrated by 51Cr release from labelled CRBC, was markedly increased by treatment with OD-432. The delayed footpad reaction to CRBC was significantly augmented by treatment with OK-432. These results in mice indicate that OK-432 can enhance the cellular immune responses which require the contribution of T cells. Such an activation of T cells by OK-432 was observed in the humoral immune response to a trinitrophenyl group. Augmentation of anti-hapten antibody production, suggesting the enhancement of helper T cell activity by OK-432, was noticed after immunization with trinitrophenyl conjugated to erythrocytes. Furthermore, this enhancement of helper T cell activity by OK-432 was confirmed by utilizing an adoptive transfer system. These results support the possibility that T cell activation may be one of the important effects of OK-432 as an immunopotentiator.

Adjuvants, Immunologic↗