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Biomedical subjects

M Toga

Publications and source records attributed to M Toga.

At least 109 records · Page 6Linked to original sources
PubMed1978

Routine analysis of multiple steroid receptors in human breast cancer.--I--Technological features.

The technological features of multiple and simultaneous assays of steroid receptors (estrogen and progestagens) have been considered in human mammary carcinomas. Most of the parameters involved in the accuracy and the fiability of S-R determination have been investigated : the stability of S-R proteins (collection and storage of sample, cytosol preparation and buffer components, endogenous steroid problem), the specificity and precise quantitation of the binding (conditions of incubations, use of steroid analogs, dextran-coated charcoal method and treatment of binding data by the Scatchard analysis), the identification of the binding (vertical sucrose density gradients) and the interferences in DCC assays. The routinely adopted protocol has been experienced in 650 cases of S-R assay. It is concluded that when performed under appropriate and properly controlled conditions and even in a small sample, multiple S-R assay could result in a precise quantitation and identification of the pathological state of the S-R system in a tumor at time of surgery. Considered with regards to actual knowledges in cell biology, that is of importance and could provide even more information about therapeutic guidance.

Breast Neoplasms↗
PubMed1978

[Human mammary carcinoma: relationship between steroid hormone receptors and histopathology. An hypothesis, the prognostic value of hormone receptors (author's transl)].

In 318 cases of human primary breast carcinoma, the presence and content of estrogen receptor (ER) have been correlated to histopathological features of these carcinomas. We have evidenced that: a) there is a relationship between the ER presence, the histoprognostic grade and the histological type; b) there is no correlation between the frequency of ER presence, the neoplasic cellularity and the stromal reaction. The presence of ER appears to be related to a differentiated state of the carcinoma and to a moderate neoplasic activity. These data show that ER, correlated with histopathological features, could be used as an estimation's factor of the cellular activity of a human mammary carcinoma, thus as a factor of better prognostic value for the evolution of this tumor.

Adult↗
PubMed1977

Giant axonal neuropathy. Involvement of peripheral nerve, myenteric plexus and extra-neuronal area.

A case of giant axonal neuropathy in a 8 years old child is reported by light and electron microscopy. Clinically, this case is strikingly similar to the rare previous reports and characterized by a distal neuropathy, CNS symptoms and tightly curled hair. Giant axons were found in the sural nerve but had been absent at the onset of the illness. An increase in the number of neurofilaments was found in the axons and neurons of the myenteric plexus. The number of microfilaments was also increased in various types of cells namely Schwann and endothelial cells and fibroblasts: This suggests that the metabolic disorder, probably inborn and genetic, does not only affect the nervous system.

Axons↗
PubMed1977

[Transformation, in vitro, of cerebral hamster cells by polyoma virus].

A cell line called HCxPy was obtained in vitro by transformation of dissociated hamster brain cell cultures by polyoma virus. The first foci of transformed cells became evident 90 to 120 days after viral infection. This cell line is now at the 46th passage. The cells appear tumorigenic for hamsters after subcutaneous and intracerebral injection. They carry the polyoma virus T and cell surface antigens. Good evidence for astrocytic differentiation can be found by morphological examination of the tumours and of the cultured cells.

Animals↗
PubMed1977

Granular cell myoblastoma.

Electron microscopic observations in three cases of granular cell myoblastoma revealed that this tumor is made up of two types of cells. 1) Granular cells grouped into nests, surrounded by a basement membrane, and displaying characteristic inclusions. Numerous processes were observed close to these cells, showing filamentous and microtubular components lying parallel to their axes. 2) Satellite fibroblasts which included angulated bodies. The relationship between these two types of cells is discussed.

Basement Membrane↗
PubMed1976

[Olfactory esthesioneurocytoma: ultrastructural study of a case (author's transl)].

One case of malignant tumour of the left nasal cavity is reported in a woman 56 year old, affected by the disease 24 years. Numerous recurrences appeared and various histological diagnoses were performed. At the last surgery, the tumour invaded the ethmoid and was a typical olfactory esthesioneurocytoma. By electron microscopy, mature ganglion cells with dense cored vesicles (neurosecretory granules) were densely packed. Neuritic processes with microtubules were rarely normal in size and their content was most often abnormal; furthermore dystrophic axons were noted in great number.

Axons↗
PubMed1976

[Immunologic blood-brain barrier in the polyoma virus--Syrian hamster system].

The authors report premilinary results of an experiment on permeability of the blood-brain barrier (BBB) to anti-tumor virus-induced immunological factors in the polyoma virus/Syrian Hamster system. The animals were protected by subcutaneous or intracranial injections with virus before challenge with polyoma virus transformed cells by both routes. BBB seemed to be permeable to the efferent part of the subcutaneously induced immune reaction. On the contrary, antigenic information introduced in the central nervous system was trapped inside the BBB. Thus the BBB might offer a "one-way" permeability in this system.

Animals↗
PubMed1976

[Niemann-Pick disease (Crocker's type C): ultrastructural study of a case (author's transl)].

The authors report electron microscopic findings in brain, bone marrow and liver biopsies in a case of juvenile Niemann-Pick disease (Crocker's type C). The diagnosis was supported by clinical data increase of blood sphingomyelin and vacuolated histiocytes in bone marrow and liver. Neurons and glial cells were filled with two types of cytosomes: classical multilamellar bodies and unusual pleiomorphic bodies. The latter type probably showed some lipofuscinic component. The relationship between type C and classical Niemann-Pick disease is discussed.

Bone Marrow↗
PubMed1976

[Cerebral hemangiopericytoma. Ultrastructural study of one case].

The authors report electron-microscopic observations upon a primitive cerebral haemangiopericytoma. The vascular appearance of the tumour is due to the presence of abundant extracellular material which has a structure like that of vascular basement membranes. The fact that the tumour cells are pericytes is confirmed by the existence of intracytoplasmic microfilaments of 60-80 A in diameter, sometimes gathered into osmiophilic aggregations and forming simple cellular junctions (zonulae adherentes). Stress is laid upon the importance of differentiating this rare tumour from an angioblastic meningioma; the haemangiopericytoma is more rapidly growing and carries a more serious prognosis.

Adult↗
PubMed1976

[Tardive spino-cerebellar degeneration with amyotrophia, complicating a severe case of pallido-luysian degeneration and diffuse histological lesions of senility. (Anatomo-clinical study of a case with nosographic discussion)].

Anatomo-clinical findings are reported in a case of central nervous system degeneration which began at age 47 and progressed over a three year period. Information obtained prior to the patient's death suggested probable spinocerebellar degeneration with amyotrophies. Postmortem anatomical examination confirmed this diagnosis but also revealed the existence of unsuspected lesions. These clinically non-manifested lesions involved severe pallido-luysian degeneration as well as numerous and diffuse senile plaques. The nosological implications of this case are discussed.

Axons↗
PubMed1975

[Atypical juvenile neurolipidosis. Ultrastructural study of a cerebral biopsy].

Electron-microscopic findings on a brain biopsy in a 9-year-old male affected by juvenile lipidosis are reported. Two types of neuronal cytosomes were discribed: lamellar concentric bodies, resembling membranous cytoplasmic bodies, and polymorphous complex bodies, unit membrane bound including various material, mostly resembling lipofuscin. The same storage was found in glial and endothelial cells. Neurochemical data failed to pin-point any specific abnormalities. The present case cannot be classified in the category of neuronal ceroid-lipofuscinosis but seems similar to certain reported cases of atypicalneurolipidosis.

Bone Marrow↗
PubMed1975

[Ultrastructural study of a chordoma].

An ultrastructural study of a case of sacral chordoma is reported. The cell type was monomorphic. The epithelial nature and the secretory function of the tumour cells was obvious in view of their content of desmosomes and the presence of mucopolysaccharide in the ergastoplasm and in the extracellular space. Because of the lack of intracytoplasmic vacuoles in the case reported, the authors doubt the individuality of the so-called "giant physaliphorous cell". The authors consider that the vacuolar appearance of the tumour cells is due to cytoplasmic invaginations caused by the enlarged extracellular space containing acid mucopolysaccharides or by processes of histiocytes containing the same material.

Aged↗
PubMed1974

Concentric laminated bodies in muscle pathology.

Concentric laminated bodies are described in five cases (one case previously reported and four new ones) of various muscle diseases. A general review of previous reports on these structures is made. They appear to be unspecific bodies generally noticed in abnormal skeletal muscle in various disorders; nevertheless they were found in normal muscle fibres of the mothers of children affected by myopathy. They have a complex filamentous structure. According to the authors, their myofilamentous nature is uncertain but seems more probable than a mitochondrial or lipidic one.

Biopsy↗