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Biomedical subjects

M Terada

Publications and source records attributed to M Terada.

At least 451 records · Page 25Linked to original sources
PubMed1989

Furosemide directly stimulates prostaglandin E2 production in the thick ascending limb of Henle's loop.

Studies were conducted to investigate direct effects of loop diuretics on prostaglandin E2 (PGE2) production using microdissected nephron segments. At first, the effect of indomethacin on the diuretic response to furosemide was re-evaluated in anesthetized rats. Indomethacin significantly attenuated the diuretic, natriuretic and chloruretic effects of furosemide without significantly affecting inulin and p-aminohippurate clearance or filtration fraction. But, in nondiuretic states, indomethacin had no significant effects on these parameters. Furosemide, ethacrynic acid and bumetanide significantly increased PGE2 production in cortical and medullary thick ascending limbs of Henle's loop (P less than .001), but not PGE2 production in the cortical and outer medullary collecting tubules. The effect of furosemide on PGE2 production in CTAL was dose-dependent, and higher concentrations of of furosemide than 10(-6) M significantly increased PGE2 production. On the other hand, chlorothiazide showed no PGE2 productive stimulation in these four nephron segments. This study demonstrates that the enhanced PGE2 production in the thick ascending limb of Henle's loop by furosemide and other loop diuretics is one possible mechanism of these drugs.

Animals↗
PubMed1989

In vivo efficacy of levamisole against larval stages of Angiostrongylus cantonensis and A. costaricensis.

Anti-larval effects of levamisole were examined on A. cantonensis in rats and A. costaricensis in mice. 1) In rats inoculated with 40 infective larvae of A. cantonensis: Compared with a non-treated control group, a significant reduction in number of worms recovered was seen in the group receiving a single dose of 1.0 mg/kg or more. A significant decrease in host lung-body weight ratio was seen in the group receiving drug of 3.0 mg/kg or more. 2) In mice inoculated with 20 infective larvae of A. costaricensis. In the non-treated control group, a severe loss in body weight and death of host animals were observed. A single dose of 30 mg/kg on 3, 4 or 5 days post-infection remarkably inhibited these changes. At 30 mg/kg for 3 or 7 days levamisole was more effective than a single dose of the drug. These results suggest that levamisole has conspicuous in vivo effects against larval stages of A. costaricensis as well as A. cantonensis.

Administration, Oral↗
PubMed1989

[A case of emergency replacement of SAM disk valve prosthesis for disk detachment].

A 47-year-old patient had undergone the mitral valve replacement using a SAM disk prosthesis in 1977. 15 years and 10 months after the first operation, she successfully underwent emergency replacement with a St. Jude Medical valve prosthesis because of detachment of the disk of the SAM prosthesis and now she is doing well.

Emergencies↗
PubMed1989

HST1 and INT2 gene coamplification in a squamous cell carcinoma of the gallbladder.

The HST1 gene has previously been found to be amplified in over 40% of squamous cell carcinomas of the esophagus. We performed Southern blot analyses on squamous cell carcinomas of the lung, nasal cavity, uterine cervix and gallbladder, using HST1, INT2 and five other oncogenes as probes. The HST1 and INT2 genes, both of which were mapped to chromosome 11 at band q13, were coamplified in a squamous cell carcinoma of the gallbladder. The degree of amplification exceeded eight fold.

Blotting, Southern↗
PubMed1989

[Modified Fontan procedure on 106 cases: indication and surgical results].

Since 1974, we have performed modified Fontan procedure on 106 patients, ranging in ages from 1 to 32 years, consisting of 44 cases of tricuspid atresia (TA), 21 with univentricular heart (UVH) of right ventricular type, 18 with UVH of left ventricular type, for which ventricular partition was unfeasible, and 23 with various complex anomalies. Hospital mortality rates for TA and other complex anomalies were 11.4 and 11.3%, respectively. Surgical results have markedly improved recently. Since 1986, 50 cases underwent Fontan procedure with 3 hospital deaths (6.0%). Late death occurred in 4 cases in a mean follow-up period of 49 months. Regarding the indication for operation, majority of patients had 2 to 3 parameters which were out of 10 criteria for Fontan procedure. Regurgitation of atrioventricular valve was repaired by annuloplasty in 19 patients underwent Fontan procedure and 17 survived. Abnormal systemic venous connection was seen in 11 cases and all survived. Association of total anomalous pulmonary venous connection is still a difficult problem and 2 of 5 cases died. Fontan procedure was performed in 8 patients following palliative right ventricular outflow reconstruction for poor development of pulmonary artery and 7 survived. Cumulative mortality rate for the entire series was relatively well at 15.1%.

Adolescent↗
PubMed1988

Differential expression of two homologous and clustered oncogenes, Hst1 and Int-2, during differentiation of F9 cells.

HST1 (or HSTF1 in human gene nomenclature) transforming gene encodes a novel heparin-binding growth factor which has 40-50% homology with fibroblast growth factors and mouse Int-2 protein. Expression of mouse Hst1 or Int-2 is rare in adult tissues, but both of them are transcribed in embryos. We found that mouse Hst1 and Int-2, like their human counterparts, were located close to each other on the genome: the distance was less than 20 kbp. Hst1 was expressed in an undifferentiated mouse teratocarcinoma cell line, F9. Upon induction of differentiation of F9 cells, the amount of Hst1 transcript was markedly decreased, while that of Int-2 transcripts increased concomitantly.

Animals↗
PubMed1988

Two homologous oncogenes, HST1 and INT2, are closely located in human genome.

Pulsed field gel electrophoresis and Southern blot analysis showed that the human oncogenes, HST1 and INT2, which code for proteins homologous to fibroblast growth factors, are less than 45 kb apart on the long arm of chromosome 11. Moreover, analysis of two overlapping cosmid clones, one containing INT2 and the other HST1 sequences, showed that HST1 is located about 35 kb downstream of INT2 in the same transcriptional orientation. The observed close proximity of the INT2 and HST1 genes may provide important insight on the origin and regulation of expression of these related genes.

Blotting, Southern↗
PubMed1988

Increasing incidence of hepatocellular carcinoma possibly associated with non-A, non-B hepatitis in Japan, disclosed by hepatitis B virus DNA analysis of surgically resected cases.

At the National Cancer Center Hospital in Japan, the total number of surgically treated hepatocellular carcinomas (HCCs) has been increasing steadily and rapidly over the last 10 years, whereas the number of cases positive for hepatitis B surface antigen in sera (HBsAg) has remained almost stable. Thus, the relative percentage of HCC cases with serum HBsAg has shown a marked decrease. In order to examine whether this increased proportion of HBsAg-seronegative patients carries hepatitis B virus (HBV) DNA in the liver, we extracted DNA from the formalin-fixed and paraffin-embedded cancerous and noncancerous liver tissues of 79 patients with HCC. The HCCs examined included 49 specimens resected during a period from 1970 to 1980 and 30 resected in 1986 and 1987. We were able to detect reliably the presence of HBV DNA by dot-blot hybridization. The presence of HBV DNA in liver tissues showed a good correlation with positivity for serum HBsAg in both examined groups. In total, HBV DNA was detected in 81% (21 of 26) of HBsAg-seropositive cases and in only 8% (4 of 53) of HBsAg-seronegative cases, indicating that the increased number of HBsAg-seronegative cases had no HBV involvement. Among these HBsAg-seronegative HCC patients, 89.7% showed a histology of cirrhosis or chronic active hepatitis in the noncancerous liver and 29.1% had a history of blood transfusion. These results suggest an increasing incidence of non-A, non-B hepatitis-associated HCCs in Japan and the possible transmission of factors by means other than blood transfusion.

Carcinoma, Hepatocellular↗
PubMed1988

Expression of the HST1 oncogene in human germ cell tumors.

HST1 (or HSTF1 in human gene nomenclature) is a transforming gene isolated from several cancerous and noncancerous cells. The HST1 protein is a heparin-binding growth factor with significant homology with human fibroblast growth factors and the mouse Int-2 protein. Here, we report the identification of expression of HST1 in a human teratoma cell line and in 5 out of 9 surgically resected human testicular germ cell tumors including seminomas and embryonal carcinomas. Mouse HST1 homologue was expressed in a certain stage of mouse embryo but not in postnatal mice.

Animals↗
PubMed1988

Expression of genetically determined diabetes and insulitis in the nonobese diabetic (NOD) mouse at the level of bone marrow-derived cells. Transfer of diabetes and insulitis to nondiabetic (NOD X B10) F1 mice with bone marrow cells from NOD mice.

The development of autoimmune diabetes in the nonobese diabetic (NOD) mouse is controlled by at least three recessive loci, including one linked to the MHC. To determine whether any of these genetic loci exert their effects via the immune system, radiation bone marrow chimeras were constructed in which (NOD X B10)F1-irradiated recipients were reconstituted with NOD bone marrow cells. Unmanipulated (NOD X B10)F1 mice, or irradiated F1 mice reconstituted with F1 or B10 bone marrow, did not display insulitis or diabetes. In contrast, insulitis was observed in a majority of the NOD----F1 chimeras and diabetes developed in 21% of the mice. These data demonstrate that expression of the diabetic phenotype in the NOD mouse is dependent on NOD-derived hematopoietic stem cells. Diabetogenic genes in the NOD mouse do not appear to function at the level of the insulin-producing beta cells since NOD----F1 chimeras not only developed insulitis and diabetes but also rejected beta cells within pancreas transplants from newborn B10 mice. These data suggest that the beta cells of the NOD mouse do not express a unique antigenic determinant that is the target of the autoimmune response.

Animals↗
PubMed1988

Infrequent loss of chromosomal heterozygosity in human stomach cancer.

By molecular genetic approach using polymorphic DNA markers which detect allelic deletion at specific chromosomal loci, we analyzed 30 human stomach cancers for possible loss of chromosomal heterozygosity. We analyzed 25 loci on 18 different chromosomes covering regions frequently deleted in several types of cancers. Loss of chromosomal heterozygosity was observed only in five of 30 cases examined, and it was infrequently detected at 10 loci on seven different chromosomes including chromosome 1 in two of 12 cases, chromosome 12 in one of four cases and chromosome 13 in three of 27 cases. It was also observed at loci on chromosomes 11, 14, 16, and 19 with very low frequency (less than 10%), but not on other chromosomes: chromosomes 3, 5, 6, 9, 10, 15, 17, 18, 20, and 22. Thus, in human stomach cancer, loss of heterozygosity occurs infrequently even at chromosomal loci often deleted in other types of cancers.

Alleles↗
PubMed1988

Cloned hst gene from normal human leukocyte DNA transforms NIH3T3 cells.

The hst gene was originally identified as a transforming gene in DNAs from stomach cancers and a noncancerous portion of stomach mucosa by transfection assays using NIH3T3 cells (1,2). Subsequently, the hst gene obtained directly from leukocyte DNA of a leukemia patient was sequenced (3,4). Here, cosmid clones containing the hst gene were isolated directly from normal human leukocyte DNA and from T361-2nd-1 cells, a secondary transformant of NIH3T3 cells induced by transfection of DNA from a stomach cancer. All clones containing the hst gene from these different sources transformed NIH3T3 cells with similar efficiency. Restriction map of the hst gene from normal leukocyte DNA was identical with that from leukocyte DNA of a leukemia patient, while the hst gene from T361-2nd-1 cells was rearranged at the 168th nucleotide upstream of the TATA box.

Adult↗
PubMed1988

A new prostaglandin E1 analogue (TFC-612) prevents a decrease in motor nerve conduction velocity in streptozocin-diabetic rats.

A new prostaglandin E1 analogue (TFC-612) was orally given to streptozocin-diabetic rats for 4 weeks after the induction of diabetes and its effects on motor nerve conduction velocity were studied. The compound significantly prevented a decrease of the velocity but did not reverse abnormal sorbitol and myo-inositol contents of the sciatic nerve. The results suggest that TFC-612 has a potent effect on diabetic nerve dysfunction via other mechanism than the correction of sorbitol and myo-inositol metabolisms and could be a potential compound for therapy of diabetic polyneuropathy.

Alprostadil↗
PubMed1988

Clonal origin of atypical adenomatous hyperplasia of the liver and clonal identity with hepatocellular carcinoma.

We present details of two separate nodular lesions of atypical adenomatous hyperplasia, within one of which a small area of overt hepatocellular carcinoma was detected. The patient was positive for serum hepatitis B surface antigen, and Southern blot analysis revealed that deoxyribonucleic acid from the lesions of hepatocellular carcinoma and surrounding atypical adenomatous hyperplasia showed the same restriction pattern of integrated hepatitis B virus deoxyribonucleic acid, indicating that the two lesions were derived from an identical clone. It was also indicated that the two separate lesions of atypical adenomatous hyperplasia were independently derived through clonal expansion of different cells.

Blotting, Southern↗