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Biomedical subjects

M Taylor

Publications and source records attributed to M Taylor.

At least 451 records · Page 25Linked to original sources
PubMed1993

Characterisation of UGP and its relationship with beta-core fragment.

Urinary gonadotrophin peptide (UGP) was originally identified by immunoassay in the urine of patients with various types of cancer and by immunohistochemistry in human cancers of various histological types. Extracts of normal adult male urine also contained UGP by immunoassay. Purified UGP from different starting material was subjected to high pressure liquid chromatography (HPLC) prior to defining amino acid sequences. Chromatographed UGP after HPLC showed three distinct fractions. The N-terminal sequence of peptide 2 was completely homologous with the beta-core fragment of human chorionic gonadotrophin (hCG) and this was found associated with two smaller peptides. The N-terminal sequence of peptide 1 has not been described previously whilst the N-terminus of peptide 3 that was sequenced showed complete homology with the N-terminal sequence of eosinophil derived neurotoxin and non-secretory ribonuclease. The monoclonal antibodies 2C2 and 6D3 only bind beta core-fragment (peptide 2) whilst the polyclonal (rabbit) antibody AK12 could bind all three peptides. The radioimmunoassay system using AK12 could be inhibited by all three peptides and the immunoradiometric assay although based on a capture antibody (2C2) that only bound peptide 2, had the potential to measure all three peptides (when bound together as UGP) at the second step when 125I-AK12 was introduced as the detector. A specific radioimmunoassay for peptide 3 was generated using 125I-peptide 3 and the AK12 antibody. Beta core-fragment on iso-electric focusing was found to have a pI > 9.5, peptide 3 showed two bands at pI = 3.5 and 3.8 whilst insufficient purified peptide 1 was available to determine its iso-electric point. Bioassay studies on UGP showed that any biological activity could be attributed to trace contamination with hCG.

Amino Acid Sequence↗
PubMed1993

The prevalence of antibodies to the human T lymphotropic virus (HTLV) in Ghana, West Africa.

We conducted a population-based serosurvey of urban areas and rural regions of southern Ghana, West Africa. Subjects (3763) of all ages were enrolled from 25 city and village sites and in studies of groups of special interest. "Positive" results were difficult to define because of a high frequency of results that were indeterminate on immunoblotting, the current standard for confirmation of HTLV-I. However, polymerase chain reaction results and HTLV type-specific discriminatory tests proved HTLV-I was present in Ghana. No HTLV-2 positivity was observed. By using strict criteria that considered indeterminate results as negative, the overall prevalence was found to be between 1 and 2% in all areas, with no difference by geographic location. Prevalence rose with age and was higher in adult women than men. However, in substudies of selected populations, we found HTLV prevalence among 124 persons with lymphomas and hematological malignancies was not different from that in the general population. Furthermore, the prevalence in prostitutes was similar to that in the general population and in pregnant women. HTLV-I is present in West Africa, but we were unable to associate HTLV-I seropositivity with malignancy or with prostitution.

Adolescent↗
PubMed1993

Cyclosporin A prolongs human hair growth in vitro.

We studied the effect of cyclosporin A on human hair growth using a recently described model in which isolated hair follicles are grown in vitro. Cyclosporin A had no effect on the rate of hair growth, but at 10(-7) M, a dose within the therapeutic blood range, it maintained hair growth for longer than control to give a 42% greater mean follicle elongation after 15 d (p < 0.05). Eighteen of 42 cyclosporin-treated follicles (43%) were still growing after 15 d compared with one of 42 control follicles (2%). These results suggest that the hypertrichotic action of cyclosporin A may be due to prolongation of the anagen phase of the hair-growth cycle.

Cyclosporine↗
PubMed1993

Evaluation of reliability and reproducibility of dental indices.

Evaluation of periodontal therapy involves the use of several oral indices to describe the health status of hard and soft tissues. It was the objective of the present study to evaluate the reliability and reproducibility of some of these indices. A calibration and standardization session was designed to calibrate 10 examiners and a "gold standard" (an experienced examiner) in evaluating the following indices: the Volpe-Manhold calculus index (VM), the Lobene stain index (SI), a non-invasive modification of the Löe-Silness gingival index (GI), the papillary bleeding score (PBS) of Loesche, and the plaque index (PI) of Quigley-Hein as modified by Turesky. For each index, the average intraclass correlation was calculated between two subject visits. The highest intraclass correlation, 0.94, was found for PBS. The intraclass correlation for PI was 0.70 and for VM, 0.65. The lowest intraclass correlations were for stain, 0.47, and GI, 0.25. Intra-subject correlations between the 2 visits were good for all indices, but were best for PBS, followed by VM. PBS seems to be the most reliable index (both intra- and inter-examiner) for measuring the oral health status and is therefore recommended for use in clinical studies.

Analysis of Variance↗
PubMed1993

Steroid prescribing for asthmatics: relationship with Asthma Symptom Checklist and Living with Asthma Questionnaire.

Psychosocial consequences of asthma were assessed for 42 patients attending an out-patient clinic using the Asthma Symptom Checklist (ASC), a five-dimensional scale of experience during asthma attacks, and the Living with Asthma Questionnaire (LWAQ), a unidimensional scale of experience between attacks. Although all subscales of the ASC and the LWAQ correlated with the physician's prescription of steroids, multiple regression showed that 32 per cent of the prescription decision variance was accounted for by two dimensions of the ASC, panic-fear and irritability. The LWAQ did not explain additional prescription variance, indicating that the physician's decisions were influenced by psychological reactions during asthma attacks rather than between attacks. Prescription decisions were not influenced by clinic-measured respiratory function. The results show some differences with previous studies; implications for patient management are discussed.

Adaptation, Psychological↗
PubMed1993

Use of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside for the isolation of beta-galactosidase-positive bacteria from municipal water supplies.

A new medium, mX-Gal, has been developed for the membrane filter enumeration of beta-galactosidase-positive bacteria in municipal water supplies. mX-Gal medium contains the chromogenic beta-galactosidase substrate 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). All Aeromonas, Citrobacter, and Enterobacter strains isolated from raw water on mX-Gal medium were beta-galactosidase positive. In contrast, only 10 to 20% of these strains produced a red colony with a metallic sheen on m-Endo agar LES medium. Of 674 chlorinated water samples analyzed for total coliforms on m-Endo agar LES medium and for beta-galactosidase-positive bacteria on mX-Gal medium, 18 that were negative for coliforms on m-Endo agar LES showed beta-galactosidase-positive bacteria on mX-Gal. Of a total of 50 beta-galactosidase-positive bacteria isolated from these samples, 76% were identified as Aeromonas hydrophila.

Aeromonas↗
PubMed1993

SPECT imaging in the diagnosis of meniscal tears.

Sixty patients scheduled for arthroscopy of a knee because of suspected meniscal tears had preoperative planar and single photon emission computed tomography (SPECT) radionuclide scans. A crescentic pattern of uptake on the SPECT transaxial view was used as a criterion for diagnosing a tear of a meniscus. The sensitivity of the investigation was 77%, specificity 74%, negative predictive value (NPV) 65%, positive predictive value (PPV) 83%, and accuracy 76%. Tears of the posterior horn of the cartilage were also associated with intense focal uptake on the transaxial view as well as increased equilibrium activity in the adjacent femoral condyle. When these appearances were included as additional criteria the sensitivity rose to 90%, NPV 81%, and accuracy 84%. This study confirms the usefulness of SPECT imaging in identifying meniscal tears.

Adolescent↗
PubMed1993

Finite element analysis of poor distal contact of the femoral component of a cementless hip endoprosthesis.

The difficulty of achieving good distal contact between a cementless hip endoprosthesis and the femur is well established. This finite element study investigates the effect on the stress distribution within the femur due to varying lengths of distal gap. Three-dimensional anatomical models of two different sized femurs were generated, based upon computer tomograph scans of two cadaveric specimens. A further six models were derived from each original model, with distal gaps varying from 10 to 60 mm in length. The resulting stress distributions within these were compared to the uniform contact models. The extent to which femoral geometry was an influencing factor on the stress distribution within the bone was also studied. Lack of distal contact with the prosthesis was found not to affect the proximal stress distribution within the femur, for distal gap lengths of up to 60 mm. In the region of no distal contact, the stress within the femur was at normal physiological levels associated with the applied loading and boundary conditions. The femoral geometry was found to have little influence on the stress distribution within the cortical bone. Although localized variations were noted, both femurs exhibited the same general stress distribution pattern.

Biomechanical Phenomena↗
PubMed1993

The parent trap.

Explore the source record for details and available documents.

Child↗
PubMed1993

Hypertension education: patient knowledge and satisfaction.

Education of patients with various diseases, including hypertension, usually occurs at an individual level through a patient's doctor as well as via various media campaigns which also includes general community education. To evaluate their effects, the knowledge of 84 patients with hypertension was evaluated by a simple 13 question true/false questionnaire and the results compared with 58 normotensive subjects matched for sex, age and educational status. A significant difference in knowledge about hypertension could not be elicited between the two groups even when compared for sex or educational status. However, subjects > 70 years of age (hypertensives and controls) demonstrated a lower level of knowledge (P < 0.05). Further questioning of the hypertensive group elicited a high level of satisfaction with their doctor's educational skills (68%) and 57% were satisfied with their own knowledge. Nevertheless, 70% requested more information about their disease. These results suggest a reasonable level of knowledge within the general community presumably reflecting community education programmes. While hypertensive patients were not more knowledgeable under the test conditions, they were reasonably satisfied with their knowledge which they stated came primarily from their doctor. Nevertheless, these findings question the effectiveness of some current patient education initiatives.

Female↗
PubMed1993

Universal precautions in the operating department.

Those operating theatre personnel who aim to keep up to date by reading the professional journals may well be confused and feel uninformed about the concept of universal precautions. While some authors focus on universal precautions, they do not mention operating theatres. Others continue to discuss the Human Immunodeficiency Virus (HIV), the Hepatitis B Virus (HBV) and high risk patient's. Furthermore the practical aspects of the implementation of universal precautions have not, to date, been thoroughly documented, other than the vague advice of 'treat every patient the same'. This article aims to clarify the concept of universal precautions, consider why they are needed, and suggest a possible pathway for their implementation. The three main changes in practice will also be discussed.

Humans↗
PubMed1992

A method of preparing blood leucocytes for flow cytometry which prevents upregulation of leucocyte integrins.

LeuCAM (CD11/CD18) cell-surface antigens are easily upregulated on cell manipulation ex vivo. A procedure for preparing leucocytes, in which human blood is immediately treated ex vivo with buffered formaldehyde and then the erythrocytes and platelets are removed by lysis and differential centrifugation, has been successfully applied to the analysis of LeuCAM antigen expression by flow cytometry. We show that the increased expression of monocyte CD11/CD18, which occurs when mononuclear leucocytes are separated by a standard Lymphoprep density gradient separation, can be avoided if cells are fixed immediately. Following this fixation polymorphs are unable to upregulate CD11/CD18 in response to fMLP stimulation in vitro. The technique produces lymphocyte, polymorph and monocyte populations that can be clearly defined on the basis of forward scatter and side scatter, and preserves the expression of various surface antigens; the percentages of gated lymphocytes expressing CD3, CD4, and CD8 were similar to those obtained using a commercial fixing and lysis solution. The processing does not render cells permeable to antibodies, as evidenced by our failure to stain cells with antibodies to intracellular antigens. We believed the method to be useful for measuring CD11/CD18 expression on blood leucocytes from normal or pathological specimens and to have application to the measurement of other cells surface antigens which may also be upregulated by the separation procedures.

Antigens, CD↗
PubMed1992

Pentamidine blocks the pathophysiologic effects of endotoxemia through inhibition of cytokine release.

Pentamidine isethionate, an antiprotozoal agent with therapeutic value against Pneumocystis carinii pneumonia, has been used for over 30 years without a precise understanding of its mechanism of pharmacologic action. We have previously reported that pentamidine has the capacity to inhibit the release of cytokines from macrophages through a post-translational processing event. The present studies were undertaken to assess the ability of pentamidine to modulate the detrimental effects of murine endotoxemia, a disease with a pathophysiology clearly linked to host-produced cytokines. Under conditions where normal B6C3F1 mice succumbed to the lethal effects of endotoxin, mice pretreated with pentamidine were significantly protected from both mortality and loss of thermoregulatory control. The EC50 for protection from mortality by pentamidine was approximately 11.4 mg/kg. These observations correlated with decreased serum levels of tumor necrosis factor (TNF) and interleukin 6. Inhibition of cytokines was not manifested as part of a generalized inhibition of protein synthesis as demonstrated by the lack of significant modulation of serum albumin in pentamidine-treated animals. In addition to decreased serum concentrations of cytokines, lungs isolated from mice treated with both pentamidine and endotoxin exhibited a decreased release of TNF compared to lungs isolated from mice treated with vehicle and endotoxin. The lower levels of TNF released from lung tissue in pentamidine-treated mice correlated with a lesser degree of alveolar deterioration than was observed in vehicle-treated mice. These data indicate that following endotoxin administration, pentamidine has a protective and antiinflammatory role both systemically and in the lung and suggest that inhibition of inflammatory cytokines may be one mechanism operable in the therapeutic activity of the drug against P carinii pneumonia.

Animals↗
PubMed1992

A modified occlusal splint to avoid tracheotomy for total midface osteotomies.

A modified occlusal splint is described which may be used to avoid tracheostomy when a total midface osteotomy is required. We demonstrate the accuracy of the modified splint by using the technique in a control subject who underwent alginate impressions, dental casts, and a face-bow transfer. A modified splint was constructed on the articulated dental models and was found to be accurate within the range of rotational movement of the temporomandibular joint. The technique is demonstrated in a patient with Crouzon's syndrome undergoing cranial vault reshaping and a monobloc (midface) advancement. The modified occlusal splint provides an alternative method of airway management when precise intraoperative occlusal relationships are important.

Child↗