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Biomedical subjects

M Tanimoto

Publications and source records attributed to M Tanimoto.

At least 235 records · Page 13Linked to original sources
PubMed1983

[M-mode and two-dimensional echocardiography in the evaluation of right atrial masses].

Five cases of miscellaneous right atrial mass were described to illustrate the very valuable diagnostic contribution of two-dimensional echocardiography (2DE). Two patients had a large myxoma in the right atrium, and other two had an extension of hepatoma into the right atrium through the inferior vena cava. The fifth patient with a past history of myocardial infarction had a floating right atrial thrombus. The myxoma in the right atrium appeared as a mottled, ovoid, and sharply demarcated mobile mass attached to the interatrial septum. The diagnosis of these two patients was confirmed at operation. The right atrial myxoma in the first case weighed 310 g and filled almost the entire right atrium and right ventricle. To our knowledge, this was the largest myxoma among previously reported cases. The hepatoma extended into the right atrium resembled myxoma, but was obscurely demarcated. The 2DE was useful to localize a large immobile mass extending into the right atrium. All these right atrial tumors were adequately demonstrated in the right lateral decubitus position with the transducer over the right parasternal position. In the fifth case, bedside real-time 2DE was performed after the attack of pulmonary thromboembolism, and an irregular echogenic mass was seen to float freely, suggesting a thrombus. Following the immediate anticoagulant therapy with heparin, the thrombus echo was no longer visible by 2DE. It was concluded that 2DE should be extensively applied to diagnose right atrial tumors or thrombi.

Adult↗
PubMed1982

Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodies.

Nine antigens systems were defined. Two were related to HLA-A,B,C and to Ia-like antigens; the others could be grouped into three categories. (i) NL-22, NL-1: NL-22 antibody reacted with leukemia cells from 12 to 16 cases of null cell acute lymphocytic leukemia (null-ALL) but not with any other type of leukemia tested or with lymphoid cells of various origins. Among cultured cell lines tested, one (NALM-6) of three null-ALL cell lines was positive, the others were negative. Absorption analysis confirmed the restriction of NL-22 antigen to null-ALL. NL-1 antibody was reactive with leukemia cells from 10 to 16 cases of null-ALL and 3 of 6 cases of chronic myelocytic leukemia in blastic crisis (CML-BC). The antigen was present also on a minor population of normal lymphoid cells. The distribution and molecular weight (100,000; glycoprotein) of the NL-1 antigen resembled that of the previously described common ALL antigen (cALL). (ii) NL-30, NL-4: Both antibodies exhibited almost identical patterns of reactivities against cultured cell lines tested. They reacted with leukemia cells from some cases of null-ALL, adult T-cell leukemia, and CML-BC, although they showed discordance in their reactivities against a panel of leukemia cells, (iii) NL-9, NL-8, NL-25: These three antibodies detect serologically distinguishable determinants on a broad range of leukemias and normal lymphoid and hematopoietic cell types. The antibodies analyzed in this study provide evidence for the heterogeneity of null-ALL by demonstrating a variety of antigen phenotypes on leukemia cells. One of the antigens (NL-22) appears to be restricted to null-ALL.

Animals↗
PubMed1982

Production of 2-Keto-l-Gulonic Acid from d-Glucose by Two-Stage Fermentation.

A practical method for the production of calcium 2-keto-l-gulonate (an intermediate in the Reichstein synthesis of l-ascorbic acid) from d-glucose has been established by using a two-stage fermentation system. d-Glucose was first converted to calcium 2,5-diketo-d-gluconate by a mutant strain of Erwinia sp. in a medium containing d-glucose, corn steep liquor, (NH(4))(2)HPO(4), and CaCO(3). After a 26-h cultivation, 328.6 mg of calcium 2,5-diketo-d-gluconate per ml was obtained, with a 94.5% yield from d-glucose. This broth was used directly for the next conversion without removal of cells by treatment with sodium dodecyl sulfate. The stereospecific reduction of calcium 2,5-diketo-d-gluconate to calcium 2-keto-l-gulonate was performed with a mutant strain of Corynebacterium sp. When the cell growth reached a maximum (about 16 h) in a medium containing d-glucose, corn steep liquor, NaNO(3), KH(2)PO(4), and trace elements, NaNO(3) was added to the culture, and then the calcium 2,5-diketo-d-gluconate broth was fed over a period of about 50 h. Since the mutant strain requires a hydrogen donor for reduction, the calcium 2,5-diketo-d-gluconate broth was mixed with d-glucose before being fed. The results of four two-stage fermentations in 10-m conventional fermentors showed that an average of 106.3 mg of calcium 2-keto-l-gulonate per ml was obtained, with a 84.6% yield from d-glucose, the starting material of calcium 2,5-diketo-d-gluconate production. Calcium 2-keto-l-gulonate was stable in the broth. Neither 2-keto-d-gluconic acid nor 5-keto-d-gluconic acid was detected in the final broth.

Journal Article↗
PubMed1982

Echocardiographic abnormalities of tricuspid valve motion in pulmonary embolism.

Echocardiographic findings in five patients with pulmonary embolism were studied. Tricuspid echocardiograms showed abnormalities in valve motion, that is a monophasic triangular wave during diastole in all the patients as well as an increased dimension of the right ventricle. An "a' dip of the pulmonary valve echocardiogram was also recognised in all five. Later tricuspid echocardiograms regained the normal M-shaped configuration. The monophasic triangular pattern of the tricuspid valve during diastole may be related to the shorter duration of tricuspid valve opening compared with that of the mitral valve. Tachycardia cannot explain this difference between tricuspid and mitral valve motion, which seems to be caused by a prolonged isovolumic relaxation time of the right ventricle resulting in a delayed opening of the tricuspid valve. These results were obtained by comparing these data with those of control subjects and patients with chronic right ventricular overloading resulting from atrial septal defect.

Adult↗
PubMed1982

Serological analysis of cell surface antigens on methylcholanthrene-induced mouse sarcomas with antisera produced against the tumors.

Out of 9 cell lines from methylcholanthrene-induced fibrosarcomas in BALB/c, 4 lines without expression of murine leukemia virus (MuLV) were selected for immunization of three different F1 hybrids with mitomycin C-treated in vivo sarcoma cells. Antibody activity was tested by immune adherence (IA) and protein A (PA) assays after the 8th immunization. Two sarcomas, CMC-11 and CMC-12, produced antibodies detectable by IA in (BALB/c x C3H/He)F1, but no antibodies were detected by PA. (BALB/c x C3H/He)F1 anti-CMC-11 was tested against CMC-11 and three other MuLV- sarcomas as well as against the skin fibroblast (C-11) from a BALB/c mouse, in which the primary CMC-11 sarcoma was induced. CMC-11 line reacted up to 1/80, but other lines were weakly positive or negative. Absorption analysis showed that the antigen defined by anti-CMC-11 was present on CMC-11 as well as on many other sarcoma cell lines, but not on BALB/c thymocytes and spleen cells, nor on leukemia and myeloma cells. Among 5 fibroblast cultures tested, C-14 showed complete absorption. It seems significant that only C-14 produced tumor nodules in BALB/c mice, in contrast to 4 other fibroblast cultures. This suggested that this antibody was directed against antigens present on transformed fibroblasts and sarcoma cells. The specificity of (BALB/c x C3H/He)F1 anti-CMC-12 serum indicated that this antiserum also seemed to detect transformation-related antigens, although it showed a broader specificity with regard to the tissue representation detected.

Animals↗
PubMed1982

The expression of HLA on two cultured choriocarcinoma cell lines.

Since gestational choriocarcinoma is believed to be derived from trophoblast, paternal antigens are expected to be present on carcinoma cells. Two choriocarcinoma lines (GCC-SV and Lu-75) transplantable in nude mice were obtained and cultures were started in order to study the expression of HLA on these tumor cells. To date, two human chorionic gonadotropin-producing cell lines, designated GCC-SV(c) and Lu-75(c), have been maintained for over two years with more than 15 passages and have been employed for serological studies. First, GCC-SV(c) (patient A2, AW30, B8, B10; husband A3, AW26, BW16, BW35) was tested by protein A assay with HLA-A typing sera and anti-beta 2 microglobulin; the phenotype was A2+, A3-, AW26-, beta 2+, which corresponded to HLA-A type of the patient. In contrast, HLA-A was not demonstrated on Lu-75(c) cells (patient A2, BW35, BW39; husband A9, AW26, BW22) by the direct test, but anti-beta 2 microglobulin serum gave a positive reaction. A mouse monoclonal antibody against HLA was also employed, but it did not react against Lu-75(c) cells. Further serological analysis was carried out by means of absorption tests, and it was confirmed that GCC-SV(c) was A2+, A3-, AW26- and Lu-75(c) was A2-, A9-, AW26-. The results so far obtained do not demonstrate the expression of paternal HLA-A antigens on choriocarcinoma cells.

Adult↗
PubMed1982

Production of monoclonal antibodies against MM antigen: the serologic identification of MM antigen with Ly-6.2 alloantigen.

Monoclonal antibodies against MM46, an ascitic mouse mammary tumor of C3H/H2, were produced by fusing mouse myeloma cell line NS-1 with spleen cells from a (BALB/c X C3H/HeN)F1 mice hyperimmunized with MM46, an MM antigen-positive tumor. Eight antibodies showed cytotoxicity against MM46, but not against MM48, an MM antigen-negative ascitic mammary tumor, and one hybridoma produced an agglutinating antibody. One of the cytotoxic monoclonal antibodies, 3-3-C, was selected, and the strain distribution and the tissue distribution of MM antigen were studied. The results demonstrated that MM antigen had a strain distribution identical to Ly-6.2 antigen, and a similar tissue distribution. Therefore, the characterization of MM antigen and Ly-6.2 antigen was investigated. Ly-6.2 antibody was shown to be cytotoxic for MM46, but not for MM48, in accordance with 3-3-C. Genetic segregation analysis of MM and Ly-6.2 antigens in 33 backcross mice demonstrated complete concordance between these two antigens. In addition, MM antigen phenotype of an Ly-6.2 congenic strain, C3H.B6-Ly-6b, was studied, and it was found to be positive in contrast to C3H/HeN. Furthermore, cross-absorption studies revealed that both MM46 cells and C3H.B6-Ly-6b lymph node cells could absorb cytotoxic activities of 3-3-C and monoclonal anti-Ly-6.2 antibody. The results so far obtained suggested strongly that these two loci controlling expression of MM and Ly-6.2 antigens were identical or very closely linked.

Absorption↗
PubMed1982

[Echocardiographic triangular pattern of the mitral valve during acute pressure overload of the left ventricle: an experimental study].

The changes of mitral valve echo and hemodynamic data [isovolumic relaxation time (IRT)/square root R-R, time constant T, peak positive dP/dt/P, left ventricular enddiastolic pressure (LVEDP) and left ventricular systolic pressure (LVSP] during acute pressure overload produced by aortic root obstruction were analyzed in 13 mongrel dogs under sodium pentbarbital anesthesia (25 mg/kg). IRT/square root R-R, time constant T, positive dP/dt and LVSP were expressed as percent changes to the value (=100%) of pre-pressure overload, LVEDP was expressed by an absolute value as mmHg. In 7 of 13 dogs, an abnormal diastolic monophasic triangular pattern of the mitral valve was observed during acute pressure overload of the left ventricle, and values of five hemodynamic data were compared between cases with or without the triangular pattern. The values of IRT/square root R-R, time constant T, positive dP/dt/P, LVSP amd LVEDP in cases with the triangular pattern became from 200 to 500% (275 +/- 100%), from 175 to 267% (220 +/- 50%), from 55 to 112% (81 +/- 21%), from 129 to 200% (59 +/- 21%) and from 7 to 33 mmHg (16 +/- 9 mmHg), respectively. The values of IRT/square root R-R, time constant T, positive dP/dt/P, LVSP and LVEDP in cases with the non-triangular pattern became from 116 to 155% (133 +/- 17%), from 116 to 154% (136 +/- 16%), from 111 to 186% (62 +/- 34%) and from 9 to 20 mmHg (9 +/- 6 mmHg), respectively. Thus, the values of IRT/square root R-R and time constant T were significantly different between the two groups. The possible explanation for the triangular pattern of the mitral valve seems to be due to impaired active relaxation system of the left ventricle resulting in a markedly delayed opening of the mitral valve. We conclude that early diastolic isovolumic relaxation of the left ventricle is impaired by acute pressure overload, and the echocardiographic diastolic monophasic triangular pattern of the mitral valve reflects this impairment.

Animals↗
PubMed1982

[M-mode and two-dimensional echocardiographic features of porcine xenograft valve dysfunction].

Clinical survey was made on the porcine xenograft valve replacements in 76 patients who underwent the replacement for the past five years at Hyogo College of Medicine Hospital. The follow-up period was from 3 to 84 months after implantation. Seventy-one patients had mitral valve replacements, 1 aortic, 2 mitral and tricuspid and 2 tricuspid. Seven patients were diagnosed as porcine valve dysfunction echocardiographically, and in four of these the dysfunction (two with bacterial endocarditis, one with perivalvular leak and one with ruptured porcine aortic valve) was confirmed at operation, and the echocardiographic features were correlated with surgical findings. M-mode and two-dimensional echocardiograms of one patient with fungal endocarditis demonstrated vegetations on the mitral and tricuspid valves. In another patient with endocarditis, the echocardiographic finding of valve thickening associated with the flail and torn cusp was observed. The two-dimensional echocardiographic study was particularly useful in detecting the dislocation of the stent echo in one patient with paravalvular regurgitation. In one patient with the ruptured and flail porcine aortic valve, the two-dimensional echocardiogram was characterized by rapid diastolic motion of the involved leaflet into the left ventricular outflow tract beyond the line of valve closure. Three patients were not confirmed at operation. In one patient, the two-dimensional echocardiogram demonstrated a systolic prolapse of the porcine mitral valve. In another two patients the M-mode echocardiographic finding included a coarse fluttering of the porcine mitral cusp in diastole. The major M-mode features of prosthetic regurgitation were fuzzy echoes with fluttering of the cusp in systole or diastole or both. In one patient with fluttering, the two-dimensional echocardiogram also demonstrated the thickening of the cusp. But in another patient with fluttering, the two-dimensional echocardiogram revealed no abnormality, and prosthetic regurgitation was not confirmed at cardiac catheterization. It was postulated that this patient had a false positive echocardiogram. Two-dimensional echocardiography complemented the M-mode echocardiographic findings and both techniques were very useful in identifying porcine valve dysfunction. Moreover, we considered that the comparison of the echocardiographic features in the course of individual case was very important in detecting porcine valve dysfunction.

Adult↗
PubMed1982

[Echocardiograms of a monophasic triangular wave of the tricuspid valve].

The echocardiographic and clinical study was performed in six patients (three acute pulmonary embolism, one for each hypertensive cardiomyopathy, ischemic heart disease and primary pulmonary hypertension) who had a diastolic monophasic triangular pattern of the tricuspid valve echogram. Left-sided and right-sided IRT / square root R-R, ICT / square root R-R, PEP, Q-Mc and Q-Tc, and PEP / ET (IRT; isovolumic relaxation time, ICT; isovolumic contraction time, PEP; preejection time, Q-Mc or Q-Tc; interval of the Q wave of the ECG to the closing point of the mitral or tricuspid valve, and ET; ejection time) were measured from echocardiograms, and the comparisons of these parameters were made between two kinds of echogram with or without triangular pattern of the tricuspid valve. There were no significant differences in the left-sided parameters between the two kinds of echocardiograms. The mitral valve echogram showed a persistent M-shaped pattern irrespective of the pattern of the tricuspid valve. Right-sided IRT / square root R-R and ICT / square root R-R were significantly prolonged and Q-Tc was significantly shortened in the echogram with a triangular pattern of the tricuspid valve. Right ventricular (RV) catheterization was performed using a Swan-Ganz catheter in four patients with the triangular pattern of the tricuspid valve echogram. The mean pulmonary artery pressure ranged from 24 to 96 mmHg (40 mmHg on an average) and RV end-diastolic pressure from 8 to 17 mmHg (12 mmHg on An average). The possible explanation for the production of the triangular tricuspid valve echogram was an impaired early diastolic relaxation and increased stiffness of the RV due to the acute pressure overloading, resulting in a delayed opening and an early closing of the tricuspid valve. We conclude that a diastolic monophasic triangular pattern of the tricuspid valve echogram is a reflection of an impaired early diastolic relaxation and an increased end-diastolic stiffness of the RV.

Adult↗
PubMed1982

[Echocardiography in superacute phase of myocardial infarction: an experimental study].

The significance and usefulness of two-dimensional echocardiography (2DE) in the evaluation of superacute phase of myocardial infarction were studied in 13 dogs with coronary occlusion, and 2DE findings were compared with the hemodynamic indices. Myocardial infarction was produced by the occlusion of anterior descending branch of the left coronary artery in 13 anesthetized adult mongrel dogs. In 6 dogs, the end-diastolic area and percent fractional shortening (%FS) in each short-axis view of the left ventricle at the level of the mitral valve, chordae tendineae, papillary muscles, low papillary muscles and apex were measured during 60 minutes, and end-diastolic wall thickness of infarct area situated in the transitional zone between the septum and the anterior wall were compared with that of non-infarct area immediately and subsequent 60 minutes after occlusion. Positive dP/dt/P, time constant T and cardiac output were measured simultaneously with an echocardiographic study. Severe enlargement and expansion of the left ventricular cavity (ballooning) and a decrease of %FS and thinning of the left ventricular wall perfused by the occluded artery occurred immediately after occlusion and persisted during subsequent 60 minutes. Time constant T was significantly prolonged, while positive dP/dt/P and cardiac output were decreased immediately and continued up to 60 minutes after occlusion. 2DE findings corresponded well with the changes of cardiac function and hemodynamics determined simultaneously. We concluded that the detection of the left ventricular ballooning is important in the diagnosis of superacute phase of myocardial infarction in dogs.

Acute Disease↗
PubMed1981

[Echocardiographic triangular patterns of the mitral valve in acute myocardial infarction: a clinical and experimental study (author's transl)].

Echocardiographic study was performed on the mitral valve echogram in cases with clinical as well as experimental acute myocardial infarction (AMI). The echocardiograms were recorded in 88 patients with AMI within 7 days from the onset. In 8 patients (9%), and abnormal monophasic triangular diastolic pattern of the mitral valve was observed. Among them, 6 were examined within 12 hours after the onset and the remainder 2 within 24 hours. In all 8, markedly prolonged isovolumic relaxation time (IRT) was present. Mitral valve motion, IRT and peak negative dp/dt of the left ventricle were examined in 5 mongrel dogs following obstruction of the main left coronary artery, one of which was followed up for next 24 hours. Following coronary occlusion, the IRT progressively prolonged from the control value of 20 msec to 130 msec (15 min), 130 msec (30 min), 140 msec (1 hr), 150 msec (6 hr) and 150 msec (24 hr), respectively. The respective values of peak negative dp/dt were 2,000, 800, 840, 840, and 620 mmHg/sec. The possible explanation for these changes was the impaired active relaxation of the left ventricle, resulting in a delayed opening (or at least the lack of usual diastolic opening) of the mitral value, which gave an abortive E-point or a markedly delayed D point. We conclude that the diastolic monophasic triangular pattern of the mitral valve echogram in a reflection of the prolonged left ventricular isovolumic relaxation, and an index of impaired left ventricular diastolic relaxation.

Aged↗
PubMed1980

Pharmacologic and clinical studies of N4-behenoyl-1-beta-D-arabinofuranosylcytosine.

Two metabolites of N4-behenoyl-1-beta-D-arabinofuranosylcytosine (BH-AC) were found in the plasma and urine, and a hydrolytic product, arabinosylcytosine (ara-C) and its deaminated product, arabinosyluraci (ara-U), were found in a preclinical study using monkeys. Of a given dose, 96% was found as ara-U and 3% as ara-C in urine in 24 h. The plasma disappearance curve of BH-AC is biphasic; the half-life of the initial phase is 40 min and that of the second phase is 120 min. At 8 h the BH-AC level is 21.9 micrograms/ml and falls exponentially to 3.6 micrograms/ml by 12 h. Ara-C was detected at the levels of 0.4-0.6 microgram/ml for 4 h. Comparative data of pharmacokinetic parameters among BH-AC, ara-C, and O2,2'-cyclocytidine showed that BH-AC had the longest plasma half-life, the smallest elimination-rate constant and the smallest excretion-rate constant. The plasma-clearance study of BH-AC in 13 patients showed essentially a pattern similar to that in monkeys; the plasma t 1/2 of 60 min in the first phase and of 180 min in the second. The BH-AC level at 2 h is 15.4 micrograms/ml, and 1.8 microgram/ml at 8 h. Initial phase I study of BH-AC was evaluated in 14 patients with leukemia and other malignancies. The starting dose was 1.5 mg/kg given as a single IV infusion for 3. The doses were when escalated up to 5.0 mg/kg. No side effects were noted with a single dose schedule. Daily consecutive infusions of 2.0 mg/kg-6.0 mg/kg for 4-21 days resulted in two patients having nausea, two anorexia, and one developing skin eruptions. Significant hematologic effects were noted with the daily infusion. One patient with acute myeloblastic leukemia achieved complete remission with 5.0 mg/kg BH-AC given daily for 21 days. It pharmacologic features, minimal toxicity, and the capability of inducing complete remission in acute leukemia indicate that BH-AC undoubtedly deserves further prospective clinical trials.

Animals↗
PubMed1980

Mixed lymphocyte-tumor cell reaction between remission lymphocytes and autochthonous leukemia cells and its relationship to the prognosis of acute leukemia.

The mixed lymphocyte-tumor cell reaction (MLTR) between peripheral lymphocytes and cryopreserved autochthonous leukemia cells was studied in 36 adult patients with acute leukemia after complete remission as a result of chemotherapy. In 20 patients the lymphocytes showed a significant blastogenic response to autochthonous leukemia cells. Eleven out of 18 patients with acute myeloblastic leukemia and 4 out of 5 with acute monocytic leukemia showed positive MLTR, whereas 5 out of 13 with acute lymphoblastic leukemia gave a positive reaction. Cryopreserved leukemia cells of the patients with not only positive but also negative MLTR were able to stimulate allogeneic lymphocytes, except in one case. The relationship between MLTR and the prognosis of leukemia was analyzed in 30 patients who were alive more than 6 months after diagnosis. No apparent correlation was observed between MLTR and the survival time from diagnosis.

Acute Disease↗
PubMed1979

Inactivation of the mitogenic property of pokeweed mitogen by erythrocytes.

In whole blood culture, pokeweed mitogen (PWM) is unable to stimulate human lymphocytes. This is because the mitogenic property of PWM is inactivated by erythrocytes, presumably due to absorption. The inactivation was observed with as few as 5 x 10(6)/ml of human erythrocytes. Therefore, when PWM is used to study the functions of human lymphocytes, especially of suppressor T lymphocytes, erythrocytes should be removed from lymphocyte preparations for accurate analysis.

Absorption↗