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Biomedical subjects

M Taniguchi

Publications and source records attributed to M Taniguchi.

At least 757 records · Page 42Linked to original sources
PubMed1983

Biosynthesis of S-(2-hydroxy-2-carboxyethylthio)-L-cysteine (3-mercaptolactate-cysteine disulfide) by the rat heart.

Incubation of 3-mercaptopyruvate with rat heart homogenate resulted in the formation of S-(2-hydroxy-2-carboxy-ethylthio)-L-cysteine (HCETC, 3-mercaptolactate-cysteine disulfide), L-cysteine and 3-mercaptolactate with the concomitant decrease in glutamate and aspartate. These results indicate that a part of 3-mercaptopyruvate was converted to L-cysteine by transamination, a part was reduced to 3-mercaptolactate, and HCETC was formed from these two products. Another peak which corresponds to L-cysteine-glutathione disulfide on amino acid analysis was also produced during the incubation.

Amino Acids↗
PubMed1983

[Monoclonal antitumor antibodies--its application to diagnosis and treatment of tumors].

The two types of monoclonal anti-melanoma antibodies (M562 and M2590) were obtained by the fusion of P3U1 myeloma cells and spleen cells of C57BL/6 mice hyperimmunized with mitomycin C-treated syngeneic B16 melanoma cells. The M2590 antibody recognized the cross-species melanoma determinant commonly shared among at least mouse, hamster and human melanoma cells, while the M562 antibody reacted with the mouse (B16) specific melanoma antigenic determinant. The M2590 antibody reacted specifically in radioimmunoassay with NP40 tumor cell extracts obtained from melanoma patients but not from other tumor patients. Thus this antibody would provide a powerful tool for immunodiagnostic evaluation of patients with malignant melanoma. Moreover, these antibodies were found to suppress the growth of melanoma cells in vivo.

Animals↗
PubMed1982

Monoclonal alloantibodies specific for the constant region of T cell antigen receptors.

We established three distinct monoclonal antibodies (7C5, 7D1; IgM and 6A4; IgG1) by the fusion of P3U1 and BALB/c (Igh-1a) spleen cells hyperimmunized with T cell blasts from the immunoglobulin heavy chain (Igh) allotype congenic CB-20 (Igh-1b) mice. The 7C5 or 6A4 antibody reacts with the constant region determinants on the antigen-binding molecule (Ct) of the antigen-specific suppressor T cell factor (TsF) or augmenting T cell factor (TaF), respectively. The 7D1 antibody, however, recognizes the shared determinants on the Ct molecules of TsF and TaF. Genetic studies of determinants recognized by these monoclonal antibodies have also suggested that the distinct Ct molecules of TsF and TaF are encoded by two discrete genes linked to the Igh-1b genes, which are located on the right side of the variable genes of Igh on the 12th chromosome. By using the immunoadsorbent columns of 6A4 antibody and anti-I-Ab, TaF, in a manner similar to TsF, was demonstrated to be composed of two chains, i.e., the Ct molecules and the I-A-encoded products. Furthermore, the Ct-bearing molecules were shown to possess the antigen-binding moiety.

Animals↗
PubMed1982

Two distinct allotypic determinants on the antigen-specific suppressor and enhancing T cell factors that are encoded by genes linked to the immunoglobulin heavy chain locus.

The alloantiserum was raised in BALB/c (H-2d, Igh-1a) mice hyperimmunized with spleen cells of Igh allotype congenic mice, CB-20 (H-2d, Igh-1b). It was found to define the new allotypic determinants (distinct from B cell Igh constant region determinants: Igh allotype) expressed only on a small population of T cells belonging to the Thy-1 dull-stained Lyt-2- or Lyt-2+ population of Igh-1b mice. Genes coding for the determinants were shown to be accommodated somewhere in the right side of the Igh variable region gene (Igh-V) cluster, as the antibody activity was completely absorbed with BAB-14 thymocytes. It was also demonstrated that the products detected by the antiserum represent the allotypic determinants (probably constant region determinants) on the antigen-binding moiety of the antigen-specific augmenting (TaF) and suppressor (TsF) T cell factors. Moreover, determinants on TsF were found to be distinct from those on TaF. Therefore, it can be suggested that the two genes coding for the T cell allodeterminants (distinct from those of the B cell Igh) are located in the right side of the B cell Igh-V on the 12th chromosome, and that both encode the antigen-recognition units of the functionally distinct T cell factors.

Animals↗
PubMed1982

The use of a monoclonal i-j-specific antibody to distinguish cells in the feedback suppression circuit from those in the contrasuppressor circuit.

A monoclonal anti-I-Jb serum designated D-7 reacts in high titer with three different T-cell subsets and one cell-free product involved in generating contrasuppressive activity, but has no activity against I-J T-cell subsets (or their cell-free mediators) involved in feedback suppression. These results give evidence for heterogeneity in the I-J subregion. They also indicate that the serological markers on I-J+ cells may define the functional activity of the regulatory circuits they belong to. Clearly, they do not separate the role that the cells play within a particular immunoregulatory circuit, i.e., inducer, transducer, or effector cells.

Animals↗
PubMed1982

In situ reconstitution of myosin filaments within the myosin-extracted myofibril in cultured skeletal muscle cells.

We studied the in situ reconstitution of myosin filaments within the myosin-extracted myofibrils in cultured chick embryo skeletal muscle cells using the electron microscope and polarization microscope. Myosin was first extracted from the myofibrils in glycerinated muscle cells with a high-salt solution containing 0.6 M KCl. When rabbit skeletal muscle myosin was added to the myosin-extracted cells in the high-salt solution, thin filaments in the ghost myofibrils were bound with myosin to form arrowhead complexes. Subsequent dilution of KCl in the myosin solution to 0.1 M resulted in the formation of thick myosin filaments within the myofibrils, increasing the birefringence of the myofibrils. When Mg-ATP was added such myosin-reassembled myofibrils were induced either to form supercontraction bands or to restore the sarcomeric arrangement of thick and thin filaments. Under the polarization microscope, vibrational movement of the myofibrils was seen transiently upon addition of Mg-ATP, often resulting in a regular arrangement of myofibrils in register. These myofibrils, with reconstituted myosin filaments, structurally and functionally resembled the native myofibrils. The findings are discussed with special reference to the myofibril formation in developing muscle cells.

Animals↗
PubMed1982

Effect of prostaglandin E1 and polyphloretin phosphate on hemolysis of human erythrocytes.

Prostaglandin E1 was found to reduce the hemolysis rate induced by various factors, such as frequent shakings, treatment with hog pancreatic phospholipase A2, the addition of active oxygens generated by a xanthine oxidase system, and the addition of a prostaglandin antagonist, polyphloretin phosphate (PPP). Prostaglandin E1 was found to act on the erythrocytes in such a way as to cause the phospholipids in the membrane to become more compactly arranged thus becoming less susceptible to the attack of hemolytic reagents. It was observed that the extents of hemolysis were different between erythrocytes from males and females and furthermore, it was shown that prostaglandin E1 clearly reduced the rate of hemolysis of erythrocytes from males, while, in females, the effects of prostaglandin E1 were less than those in erythrocytes from males.

Adult↗
PubMed1982

The role of cytotoxic T lymphocytes in the pathogenesis of Vogt-Koyanagi-Harada disease.

Peripheral blood lymphocytes (PBL) were isolated from blood of 6 patients with Vogt-Koyanagi-Harada disease (VKH), and their cytotoxic activity was assayed using 51Cr release from the labelled cells; the cytotoxicity was expressed as percentage release in reference to the complete release using saponin. The PBLs from the patients showed a significant cytotoxic activity against P-36 human melanoma cells, and the specificity of the reaction was confirmed by the cold target inhibition test and also by the use of HeLa-S3 human cervical carcinoma cells and B-16 mouse melanoma cells, since no significant cytotoxicity was seen against these cells. The cytotoxic activity was greatly reduced by pretreatment of the PBLs with monoclonal antihuman Leu-1 antibody plus the rabbit complement. It was, therefore, thought that the cytotoxic activity against the melanoma cells is mainly due to T cells. In addition, the cytotoxic activity was specifically blocked by monoclonal antihuman Leu-2a antibody, the specificity of which is distributed on cytotoxic/suppressor T cell subset of normal human peripheral lymphocytes and normal human thymocytes. A possibility was discussed as regards the involvement of the cytotoxic T lymphocytes in the pathogenesis of VKH.

Adult↗