Preparation and evaluation of antisera to oxytocin.
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Biomedical subjects
Publications and source records attributed to M Talas.
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This is a report about 20 sterile women in whom the amenorrhoea or oligomenorrhoea was accompanied by hyperprolactinaemia. Parlodel, Lysenyl, and Deprenon were used for treatment. The therapy was followed by pregnancy of 70 per cent of the women.
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The levels of HCG, prolactin, progesterone, 17-beta estradiol and oxytocinase were simultaneously tested in one serum sample in women during the normal course of pregnancy and in those with late toxaemia of pregnancy or threatened premature labour. The significance of the deviations from the mean values measured in both groups of risk pregnancies was estimated in comparison with the values found in physiological pregnancies. On the basis of the comparison of all parameters followed, it has been found out that the deviations in the hormonal levels as measured in both groups of risk pregnancies were as a rule similar.
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In women during the third trimester of normal pregnancy and at labour the level of oxytocin and progesterone in plasma were determined by means of radioimmunoassay and the activity of oxytocinase was measured spectrophotometrically. The plasma oxytocin levels measured from the 25th week of pregnancy showed a gradual increase with the peak values at the 39th and 40th week. At labour, the oxytocin values remain at the same level as these found at the end of pregnancy. The increase in oxytocin levels at contractions compared to its values found between contractions was not statistically significant. Oxytocinase levels showed also an ascending character until the 38th week of pregnancy. Later, a decrease was found which continued until the termination of pregnancy. At labour, there was a decrease in progesterone level in plasma which was about at the limit of significance.
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