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Biomedical subjects

M Takeya

Publications and source records attributed to M Takeya.

144 records · Page 8Linked to original sources
PubMed1984

Tumor immunolocalization using monoclonal antibodies which bind placental alkaline phosphatase.

Increased levels of PLAP in the serum of patients with certain types of cancer, particularly ovarian cancer and seminoma, indicate that PLAP may be a useful marker for detection of those tumors. Our goal was to further examine the usefulness of PLAP as a tumor marker by investigating the possibility that monoclonal antibodies binding PLAP may be useful for detection of the growth and metastasis of tumors which express the enzyme. Thus, we developed an experimental system where both a PLAP- positive and PLAP- negative tumor were grown in nude mice. The mice were then injected with radioactively-labeled F(ab)'2 fragments of a monoclonal antibody. The results are in accord with those of others and indicate that the monoclonal antibody localized in the PLAP-positive tumor 10 times or more often than it localized in the PLAP-negative tumor or in normal mouse tissues. Therefore, PLAP would appear to be a useful marker for the immunodetection of certain tumors in humans by external scintigraphy. PLAP may similarly be effective as a target for the delivery of toxic reagents to tumor cells in vivo using drugs conjugated to PLAP-specific monoclonal antibodies.

Alkaline Phosphatase↗
PubMed1983

Long-term polyvinylpyrrolidone storage. Histochemical and ultrastructural observations in two biopsy cases.

Biopsy specimens of lymph nodes or bone marrow taken from two cases, which seemed to have received intravenous infusions of polyvinylpyrrolidone (PVP) more than 15 years ago, were examined histopathologically, histochemically or electron microscopically. In the lymph nodes, proliferation of foam cells, often with multinuclear giant cells, and basophilic vacuolar massive deposition were found markedly in the lymphatic sinuses and sporadically in the parenchyma. In the bone marrow, basophilic foam cells predominated, together with infrequent appearance of multinuclear giant cells and basophilic vacuolar deposits. The basophilic materials stored in the foam cells and multinuclear giant cells, as well as basophilic vacuolar massive deposits, were histochemically proved to stain with the special stains for demonstration of PVP. Electron microscopically, storage inclusions observed in the cytoplasm of PVP-storing macrophages were comprised of electrondense, amorphous substances or of vacuolar, fine reticulogranular, flocculent materials and were bounded by a single delimitating membrane. Most of the inclusions, particularly osmiophilic ones, showed intense acid phosphatase activity, while larger, vacuolar inclusions had a reduced or no enzyme activity. Extracellularly, vacuolar storage inclusions with no enzyme activity were found. The possible mechanism of long-term PVP storage in the tissues was briefly discussed.

Adult↗
PubMed1981

A postmortem study of malignant glucagonoma with heart muscle hypertrophy, including chemical, histochemical, immunohistological and ultrastructural observations.

The autopsy findings in a 58 year old female with the typical glucagonoma syndrome with heart muscle hypertrophy are described. Despite remission of a skin rash and hyperglucagonemia following removal of metastatic liver tumors, the patient died of massive cerebral hemorrhage caused by uncontrolled hypertension and septic cerebral angiitis. On the basis of surgical and autopsy materials, the tumors in the pancreas and liver were defined as malignant glucagonoma by histological, histochemical, immunohistochemical, and ultrastructural studies. The pathohistological changes in several organs that might be related to the hypersecretion of pancreatic glucagon are discussed.

Adenoma, Islet Cell↗
PubMed1981

Developmentally heterotopic urogenital tissues in a retroperitoneal lipoma with hematopoiesis.

This report concerns an unusual retroperitoneal lipoma with hematopoiesis in a 49-year-old male. A unique feature is the presence of well differentiated male urogenital tissues, such as the prostatic gland, urethra and urinary bladder in part of the tumor. The process of the differentiation of these tissues is also illustrated. Although this lipoma attained a large size weighing 1,500 g, it is apparently a kind of malformation derived from the sequestered retroperitoneal Wolffian vestige. No similar cases have so far been reported in the world literature.

Cell Differentiation↗
PubMed1980

Extramedullary hematopoiesis presenting as mediastinal tumor.

An autopsy revealed extramedullary hematopoietic foci scattered along the thoracic vertebrae and between the ribs in a patient who died of lung cancer. One of them formed a tumor-like mass adhering to the 10th vertebra, and measured 8X6X2 cm. The cellular components included erythrocytic, granulocytic and megakaryocytic series at various stages of maturation as well as abundant iron-pigmented histiocytes. The patient also suffered from chronic hemolytic anemia and secondary hemochromatosis. The intimate relationship of the intrathoracic tumor-like masses of extramedullary hematopoietic tissue with chronic hemolytic anemia is briefly reviewed.

Anemia, Aplastic↗
PubMed1980

Application of the enzyme histochemical method for the isolation of HeLa variant inducibly producing the Kasahara isozyme of alkaline phosphatase.

A cloning method utilizing the enzyme histochemical procedure was applied to isolate variant HeLa subclones which produce different alkaline phosphatase isozymes. Phosphate was used to distinguish between Kasahara isozyme and Regan isozyme. The use of filter paper made it possible to determine the localization of the Kasahara isozyme-positive colonies. By this method, two variant clones, HeLa S3-10 KP and HeLa S3-10 KN, were isolated. Kasahara isozyme was induced in the former by increased cell density, but not in the latter.

Alkaline Phosphatase↗
PubMed1980

Alkaline phosphatase from two HeLa S3 subclones: one producing Regan and the other, Kasahara isozyme.

HeLa S3 cells have been found to possess heat-stable alkaline phosphatase. However, the present electrophoretic study indicated that this cell line also contained a heat-labile isozyme as a minor component with the major heat-stable (Regan) isozyme. Histochemical demonstration of these two isozymes was also made through heat treatment and inhibition tests. By the single cell cloning of HeLa S3 cells, two subclones, HeLa S3-5 with heat-labile Kasahara isozyme and HeLa S3-10 with Regan isozyme, were established. All 18 subclones from this HeLa S3-5 possessed the same type of heat-labile alkaline phosphatase. These two isozymes were expressed in two cell lines, respectively, during long term culture. The present study suggests that certain cell populations of HeLa S3 have changed their phenotype to Kasahara isozyme due to alteration of a regulatory gene during long term cell culture.

Alkaline Phosphatase↗
PubMedDate not supplied

Vascular encasement by pancreatic cancer: correlation of CT findings with surgical and pathologic results.

PURPOSE: The purpose of this study was to correlate thin-slice high-resolution helical CT findings of arterial and venous involvement in pancreatic cancers with surgical and histopathologic results. METHOD: Forty-eight patients with pancreatic cancer underwent preoperative thin-slice high-resolution helical CT, followed by surgical dissection of the pancreatic vessels during curative or palliative surgery. Major vessels running within 1 cm from the tumor margin were evaluated. CT appearance was graded on a 0-4 scale (0: none, 1: <24%, 2: 25-49%, 3: 50-74%, 4: 75-100%) by circumferential contiguity of tumor to vessels. Resected specimens were available from 26 patients. RESULTS: Surgical correlation of CT findings was available in 89 veins and 83 arteries, and both surgical and histologic correlation was available for 42 veins and 29 arteries. At surgical observation, 29 of 35 veins (82.9%) evaluated as CT grade 3 or 4 were found to be involved, whereas only 18 of 30 arteries (60%) evaluated as CT grade 3 or 4 were proved to be involved. On microscopic observation, tumor invasion to the portal venous systems was confirmed in 15 of 42 (35.7%) vessels, and this invasion was depicted as from CT grades 1 to 4. In arteries, tumor invasion was seen in 3 of 29 vessels (10.3%), all of which were graded as 3 or 4 by CT. CONCLUSION: The grading system of vascular invasion should differ between arteries and veins. Involvement of the venous system exceeding one-half circumference of the vessels (grade 3 or 4) was suggestive of vascular invasion; however, this criterion was not always satisfactory for the evaluation of tumor invasion in the arterial system.

Adult↗
PubMedDate not supplied

Emperipolesis in a case of malignant lymphoma: electron microscopic and immunohistochemical investigation.

In the tissue sections of axillary lymph nodes surgically removed from a case of malignant lymphoma (non-Hodgkin's, diffuse, large cell type), many large cells were observed to contain lymphoma cells in their cytoplasm. From the findings of light microscopy in serial sections and electron microscopy, this phenomenon was confirmed to be "emperipolesis." By immunohistochemistry, the large cells that contained lymphoma cells possessed most of the monocyte/macrophage markers, whereas the lymphoma cells revealed some B-cell markers, suggesting that they were of germinal center cell origin. In a survey of the literature, we found no report describing emperipolesis in the tissue sections of malignant lymphoma. Although the precise mechanisms and biological significance of emperipolesis in the present case are not fully understood, the existence of some interactions between macrophages and lymphoma cells is suggested.

Aged↗
PubMedDate not supplied

Production of monocyte chemoattractant protein-1 by cultured glomerular epithelial cells: inhibition by dexamethasone.

Glomerular epithelial cells (GECs) have been shown to be one class of target cells in immunological and nonimmunological glomerular injury of glomerulonephritis, some cases of which are accompanied by infiltration of glomeruli by monocytes/macrophages. In this study we tested whether GECs in culture produce monocyte chemoattractant protein-1 (MCP-1), a potential molecule responsible for monocyte recruitment in inflammation, and whether the production is inhibited by glucocorticoid. GECs were obtained from outgrowth of rat glomeruli. Levels of MCP-1 mRNA and protein were determined by Northern blot analysis and ELISA, respectively. Northern blot analysis revealed the expression of MCP-1 mRNA in cytokine-treated GECs. The expression of MCP-1 mRNA was inhibited by dexamethasone. Quantitative analysis by ELISA confirmed the production of MCP-1 protein by GECs and the inhibitory effect of dexamethasone. These results indicate that cytokine-treated GECs produce and secrete MCP-1 and that the MCP-1 production is inhibited by dexamethasone. We suggest that MCP-1 produced by GECs may play a role in the recruitment of monocytes/macrophages in glomerulonephritis and that the therapeutic effect glucocorticoid on the disease might include the inhibition of the production of MCP-1.

Animals↗