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Biomedical subjects

M Takayama

Publications and source records attributed to M Takayama.

At least 145 records · Page 8Linked to original sources
PubMed1995

[Development of the monoclonal antibody to cultured uterine endometrial adenocarcinoma cells (EI)].

A new monoclonal antibody (Moab), 3H2, was produced by immunizing Balb/C mouse with EI, a newly established human cell line from moderately differentiated endometrial adenocarcinoma of the uterus. The molecular weight of the Moab 3H2-defined antigen was 36,000 daltons, and suggests that the epitope of the Moab 3H2-defined antigen was protein moiety. Moab 3H2 was of the IgG1, subclass. Using formalin-fixed, paraffin-embedded human tissues and immunohistochemical techniques (ABC method), Moab 3H2 has demonstrated reactivity, Moab 3H2 reacted with well and moderately differentiated endometrial adenocarcinoma of the uterus, but did not react with poorly differentiated endometrial adenocarcinoma of the uterus, Moab 3H2 did not react with other tissues, malignant or normal, except ovarian clear cell adenocarcinoma, suggesting that Moab 3H2 reacts with well and moderately differentiated endometrial adenocarcinoma of the uterus with high specificity. To analyze the epitope of the Moab 3H2-defined antigen, it should be useful to make clear the function of carcinogenesis, differentiation and growth of endometrial adenocarcinoma of the uterus.

Adenocarcinoma↗
PubMed1995

[Establishment and characterization of the new cell line (EI) from a human endometrial adenocarcinoma].

A moderately-differentiated endometrial adenocarcinoma cell line(EI) was established from a surgical specimens obtained from a 55-year-old woman with endometrial carcinoma. This cell line could be transplanted to nude mice, where the cells showed the same histological type as the primary tumor. The doubling time of the cell line was 50.5 hours; the saturation density was 7.5 x 104 cells/cm2; the plating efficiency was 46%. This cell line was determined to produce TPA, but not other tumor markers, such as CA125 or CEA. Neither estrogen receptor, nor progesterone receptor was detected from the culture cell or the primary tumor. Chromosome analysis revealed that cells examined were all 46,XX, + 8,t(14q14q), and only cells with this karyotype were thought to be able to grow. From these results, it was suggested that a gene on No. 8 chromosome would be involved in the carcinogenesis of endometrial adenocarcinoma. Thus this cell line was thought to be useful for the clarification of gene conversion during the process of development of endometrial adenocarcinoma.

Adenocarcinoma↗
PubMed1995

[Stereotactic radiosurgery using a linear accelerator (LINAC): simulation and positioning].

Stereotactic radiosurgery using a Gamma unit obtains good results for small intracranial diseases, arteriovenous malformation (AVM) and acoustic neurinoma. In stereotactic radiosurgery using a linear accelerator (LINAC), many fundamental problems are to be solved. 1) accuracy of a LINAC, 2) making the collimators for high energy X-ray narrow beams, 3) dosimetry for high energy X-ray narrow beams, 4) irradiation methods for stereotactic radiosurgery, 5) fixation of a patient's head and 6) simulation of a target. The usefulness of our method for simulation of a target and for positioning for radiosurgery was investigated. High energy X-ray narrow beams obtained with the collimators for narrow beams (field sizes: 9mm phi, 18mm phi and 27mm phi) satisfy clinical requirements for stereotactic radiosurgery, as indicated by dose profiles and isodose curves. No dosimetry method for high energy X-ray narrow beams has been established yet. Of the main irradiation methods for stereotactic radiosurgery, the method using multiple non-coplanar converging arcs needs no drastic reconstruction for use with LINAC. A patient's head was completely fixed by the stereotactic frame (Patil stereotaxic system or Leksell micro-stereotactic system). Simulation of a target was performed under CT scan. On CT image, the center of a target was determined and the three-dimensional coodinate on the stereotactic head frame target was settled so that the target would be reached. The three-dimensional coordinate for the target was coincided with the isocenter of a LINA by the laser beams of three-directional pointers. Afterwards, the target position was finely adjusted, by using the target positioner manipulator system.(ABSTRACT TRUNCATED AT 250 WORDS)

Humans↗
PubMed1995

[Estimation of effective dose from CT examination].

Tissue or organ doses related to radiological risk were determined for four different types of CT scanners with a spiral scan function. Dose measurements were performed using a Rando phantom and two types of thermoluminescent dosimeters. The effective doses recommended by the International Commission on Radiological Protection in 1990 were evaluated using the tissue or organ doses determined with the phantom measurement. The resultant effective dose per CT examination ranged from 4.6 to 10.8 mSv for chest examination and from 6.7 to 13.3 mSv for upper abdominal examination. It should be noted that the effective dose from CT examination will be increased by increasing in the frequency of CT examinations and technical development of CT scanners.

Humans↗
PubMed1995

[mRNA expression and protein localization of placental tissue protein 11, 12, 19 in gynecologic malignant tumors].

In recent years, the localization and function of placental tissue proteins (PPs), extracted by Bohn et al., have been extensively studied, the genetic code has been identified for each of the PPs. The present study was carried out to clarify the mRNA expression and protein localization of PP11, PP12, PP19 at the cell level and also to define PP19 the nature of which has remained obscure. PP19 was said to be placenta-derived S-100P. PP11 mRNA was not expressed in cytotrophoblast-derived normal placental tissue or endometrium-derived normal cells, but was expressed in syncytiotrophoblast-derived normal placental tissue, and in choriocarcinoma and endometrial adenocarcinoma, suggesting its involvement in carcinogenesis. PP12 mRNA was expressed in cytotrophoblast-derived normal placental tissue or endometrium-derived normal cells, but was not expressed in syncytiotrophoblast-derived normal placental tissue, choriocarcinoma or endometrial adenocarcinoma, suggesting that this protein serves in the function of normal cells. PP19 mRNA was expressed in the squamous epithelial cells of the uterine cervix and the villous cells, PP19 was localized in more differentiated regions, where cells tended toward keratinization, in both normal and dysplastic uterine cervices. In squamous cell carcinoma, PP19 was localized in more differentiated cells with a large cytoplasm. PP19 mRNA was not expressed in normal endometrial glands, but was detected in endometrial adenocarcinoma, suggesting its involvement in cell differentiation in cervical epitherial cells.

Base Sequence↗
PubMed1995

In vivo regeneration of vestibular hair cells of guinea pig.

Recent studies have shown that inner ear sensory cells may regenerate in mammals as well as in birds. In this in vivo study we investigated the regeneration of vestibular hair cells in guinea pigs after gentamycin intoxication, and found that the vestibular sensory epithelia reproduced new hair cells 30 days after degeneration, due to 30 consecutive days of gentamycin treatment. From the regeneration pattern of sensory epithelia, the supporting cells were identified as the progenitors of hair cells.

Animals↗
PubMed1995

The most electron-donating sites in duplex DNA: guanine-guanine stacking rule.

The most readily oxidizable sites in one-electron oxidation of duplex DNA are shown to be the guanine (G) residues located 5' to guanine. Based on experimental results and ab initio calculations, G-G stacking rule has been proposed. This rule indicates the most electron-rich sites contained in B DNA and is very useful for understanding CT interactions between electron-accepting molecules and DNA.

Binding Sites↗
PubMed1994

[Effective dose evaluation of angiography staff].

The effective doses received by staff members involved in angiographic examinations were determined using data compiled on radiation doses obtained from monitoring personnel and phantom measurements. Thermoluminescence personnel monitors were used to evaluate the personal doses received by the staff during radiological procedures. Organ or tissue doses were determined by using phantom measurements in which a RANDO-woman phantom was used to simulate geometric conditions during actual angiographic examinations. The annual effective dosage received by radiologists was estimated to be 8 to 9 mSv per year when they wore a lead-rubber protector. When this protector was disregarded, their annual effective doses frequently went beyond the annual limit of 50 mSv. The surface dose to the female abdomen per ten months was estimated as 1.7 mSv. Thus, care should be taken by women staff members.

Angiography↗
PubMed1994

Reactivity of varicella-zoster virus subunit antigens in enzyme-linked immunosorbent assay to sera from varicella, zoster, and herpes simplex virus infections.

Serological responses to varicella-zoster virus (VZV) subunit antigens, such as capsid, envelope, and soluble (S) antigens, in patients with VZV and herpes simplex virus (HSV) infections were studied by comparing with responses to virion (V) antigens using an enzyme-linked immunosorbent assay (ELISA). S antigen, prepared by concentrating supernatant of VZV or HSV type 1 (HSV-1)-infected cell culture fluid, reacted strongly to sera from patients with secondary infection but reacted poorly to those from patients with a primary infection of VZV or HSV. Antibody titers to VZV-S antigen persisted for a long period in patients with VZV infections. Patients infected with VZV showed antibody increase to HSV-1, when tested by complement fixation or complement-enhanced neutralization test, in cases with a history of prior HSV infection. However, such a cross-reaction was only observed to a minor extent in ELISA test using S antigen. S antigen reaction was stronger in secondary infections in tests with various subunit antigens. Almost no cross-reactivity was observed in an immunoblotting test with S antigen. Differentiation between infections with either varicella or zoster or HSV can be made by comparison of antibody responses to V and S antigens.

Adolescent↗
PubMed1994

Superoxide dismutase inhibits interleukin-1-induced degradation of human cartilage.

Interleukin-1 (IL-1) is a key mediator in the pathogenesis of cartilage destruction in arthritis-related disorders. Human recombinant superoxide dismutase (SOD) blocked IL-1-mediated cartilage destruction but had no effect on IL-1-mediated changes in glycosaminoglycan and prostaglandin E2 synthesis. These data suggest that superoxide anions may be one factor of IL-1-mediated cartilage destruction.

Aged↗
PubMed1994

Escherichia coli cad operon functions as a supplier of carbon dioxide.

We examined the gene expression of the Escherichia coli cad operon, which consisted of the genes cadB and cadA (lysine decarboxylase), using cells possessing cadB-lacZ fusion gene. The cad operon was expressed when O2 was limited, and the expression was optimal at pH 6.3. The beta-galactosidase activity was lowered by the addition of sodium carbonate to the medium. The expression of the cad operon was reduced in cells containing the plasmid-encoding ornithine decarboxylase, which produced carbon dioxide, indicating that the gene expression of the cad operon was regulated by carbon dioxide (or its derivatives). It is known that the Krebs cycle is a major pathway for producing carbon dioxide, and that its activity is repressed when O2 is limited. Thus, our present results suggested that the physiological role of the cad operon is to supply carbon dioxide when its internal level is lowered under O2-limiting conditions at a low pH.

Amino Acid Transport Systems↗
PubMed1994

End-systolic volume and long-term survival after coronary artery bypass graft surgery in patients with impaired left ventricular function.

BACKGROUND: Left ventricular function is the main predictor of long-term survival in patients with coronary artery disease. In patients with impaired left ventricular function after myocardial infarction, end-systolic volume is a better predictor than the global ejection fraction. We analyzed long-term follow-up of patients with impaired left ventricular function undergoing coronary artery bypass graft surgery to evaluate preoperative predictors of survival. METHODS AND RESULTS: Consecutive patients with ejection fractions < or = 40% (n = 193) who had undergone surgical revascularization were followed to assess the predictive value of preoperative baseline characteristics and catheterization findings for long-term survival. Patients were followed for 133 +/- 30.7 months. At the time of surgery, patient age was 56 +/- 7.9 years and 169 patients (87.6%) had a history of previous myocardial infarction. Thirty-one patients (16%) were female. The ejection fraction was 32 +/- 7%, and the end-systolic volume was 147.4 +/- 52.6 mL. One hundred sixty-four patients (84.9%) had three-vessel disease, and 44 (22.8%) had a left main stenosis with > 50% diameter loss. Follow-up was complete in 99%. Fourteen patients died (7.3%) within the first 30 days after surgery. Twelve-month actuarial survival was 86%, 4-year survival was 80%, and 10-year survival was 40%. Predictors of poor long-term survival on multivariate analysis were end-systolic volume index (chi 2 = 14.02, P = .002), number of previous myocardial infarctions (chi 2 = 6.47, P = .001), preoperative stenosis score (chi 2 = 4.97, P = .02), and age at the time of surgery (chi 2 = 4.45, P = .03). CONCLUSIONS: End-systolic volume index is the major predictor of survival after coronary artery bypass graft surgery in patients with impaired left ventricular function. Strategies to prevent ventricular dilatation, such as angiotensin-converting enzyme inhibitors, may improve the long-term outcome in these patients.

Adult↗
PubMed1994

[Simple analysis of fatty acid in human milk by HPLC].

The conventional analysis of fatty acid requires such complicated operations as extraction of total lipid, evaporation of organic layer, alkaline hydrolysis, washing with n-hexane, re-extraction of fatty acid and washing with water, and therefore, is time-consuming. Herein, we report a simplified method dispensing with the steps of the extraction of total lipids, the evaporation of organic layer and the washing with n-hexane after hydrolysis. This method showed satisfactory results on the linearity of standard curves and their productibility, and a correlation was obtained between those obtained by the traditional method and by our simple method in human milk. We employed this method for the analysis of human milk secreted between the 3rd day and the 42nd day after delivery. The concentration of total fatty acid in human milk secreted after lactation showed a higher value than that before it, while the constituent ratio of fatty acid did not show such a great difference.

Adult↗