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Biomedical subjects

M Takami

Publications and source records attributed to M Takami.

At least 163 records · Page 9Linked to original sources
PubMed1983

[Study on postoperative local chemotherapy of malignant brain tumors using ACNU and PSK].

There have been many attempts to treat patients with malignant brain tumors represented by glioblastomas using nitrosourea (NU) derivatives such as BCNU and CCNU but the clinical results are not so remarkable compared with previous reports concerning experimental studies. The reason for an efficacy of NU derivatives in brain tumors is considered to be its higher lipid solubility which makes the drug crossing the BBB easily. On the other hand, there is some evidence that higher lipid solubility did not guarantee NU to reach always to all portions of solid tumor after systemic administration. We have performed a chemotherapy of malignant brain tumors using ACNU for five years. This drug is not only lipid but also water-soluble in some grade; therefore, the drug is administrated intravascularly and locally with ease. Nine cases of malignant gliomas were treated with local chemotherapy employing ACNU and two cases of glioblastomas are surviving now over five years and about four years, respectively. From the anatomical standpoint of view, it is considered to be necessary for local chemotherapy of brain tumors to possess some pathognomonic characters such as cyst formations, central necrosis and localized cortico-meningeal adhesions, which are rather frequently found in malignant gliomas and are suspected also easily by CT examination preoperatively. A local chemotherapy in the present study has been performed using 10 to 50 mg of ACNU through an indwelling catheter inserted during operation. No general toxicity occurred in all patients except one, who experienced purulent meningitis after long-term drainage. In our study on concentration of ACNU, intracarotid injection resulted in higher concentration in brain tumor tissue than intravenous injection, but these concentration considered to be not high enough to suppress the tumor cell growth. On the other hand, the intracavitary concentration of ACNU at 24 hr after drug administration was high enough to suppress the tumor cell growth.

Adjuvants, Immunologic↗
PubMed1982

Kinetic aspects of hemoglobin.haptoglobin-receptor interaction in rat liver plasma membranes, isolated liver cells, and liver cells in primary culture.

125I-Hemoglobin.haptoglobin injected intravenously into rats was incorporated into liver parenchymal cells as evidenced by a cell separation technique. A mixture of freshly isolated liver parenchymal and nonparenchymal cells failed to internalize and degrade the 125I-hemoglobin.haptoglobin added, although it retained the ability to bind the molecule. The liver parenchymal cells in primary culture also lacked the ability to degrade 125I-hemoglobin.haptoglobin, although they bound the molecule more extensively as compared with the freshly isolated liver cells. It was confirmed that the 125I-hemoglobin.haptoglobin which was bound to the freshly isolated liver parenchymal cells localized on the outer surface of liver plasma membranes. Scatchard plots revealed the existence of two binding sites for 125I-hemoglobin-haptoglobin on the isolated liver plasma membrane: an apparent high affinity binding site (Kd = 1.3 X 10(-7) M) and an apparent low affinity binding site (Kd = 4.0 X 10(-6) M) at 37 degrees C. In contrast, freshly isolated liver parenchymal cells had only an apparent low affinity binding site (Kd = 1.4 X 10(-6) M) at 37 degrees C. Impairment of the apparent high affinity binding site during the isolation procedure with collagenase seemed to be related to loss of the ability to internalize and degrade the 125I-hemoglobin.haptoglobin molecules into the freshly isolated liver parenchymal cells or liver parenchymal cells in primary culture.

Animals↗
PubMed1982

A growth-hormone--producing pituitary adenoma and an internal carotid artery aneurysm.

The case of a growth-hormone-producing pituitary adenoma which was in direct contact with a right internal carotid, medial-type aneurysm is reported. Direct clipping of the aneurysm and subcapsular removal of the adenoma were performed through a right frontotemporal craniotomy. The rarity of this direct contact and the cause of formation and enlargement of the aneurysm are discussed. The importance of angiography in cases of pituitary adenoma is stressed.

Acromegaly↗
PubMed1982

Malignant transformation of intrathoracic ancient neurilemoma in a patient without von Recklinghausen's disease.

A case of intrathoracic ancient neurilemoma undergoing malignant change in a 48-year-old woman is reported. The lesion was first discovered incidentally during routine roentgenologic examination of the chest 15 years before its surgical removal. The gross and histologic examination of the tumor showed the features of ancient neurilemoma with extensive regressive changes as well as small preserved areas composed of Antoni type A and B tissues. In addition, obviously histologically malignant foci manifested by the presence of increased cellularity, active mitotic activity, and gradual loss of orginary neurilemomatous differentiation were present. The patient died 16 months after operation of recurrent and distant metastatic disease. At autopsy, there was no evidence of generalized neurofibromatosis or other primary cancer. A review of the available literature revealed only two other similar cases documenting malignant transformation of solitary benign neurilemomas in patienets without von Recklinghausen's disease.

Aged↗
PubMed1982

[Postoperative treatment of malignant brain tumors with ACNU and PSK-particularly immunological follow-up research].

From the recent progress in immunological study, many authors have reported immune suppression in patients with malignant brain tumors. On the other hand, many factors are considered to influence the patterns of cellular and humoral immune parameters in patients with brain tumors, pre- and post-operatively. We treated the patients with malignant brain tumors using ACNU and PSK pre- and post-operatively, and 4 year survival rate was 35.3% (N: 17) in glioblastomas. We planned this time to measure immune parameters (Ig,lymprocyte number,Lyn and T-cell, Tn) pre- and post-operatively in order to study the immune states of patients. The results were as follows: The most significant finding was the elevated serum level of IgG in longterm survival of the patients with glioblastoma and the reduced one in patients with postoperative death. No other humoral immunocompetence existed except slightly reduced IgM level in the patients with death. Contrary to many previous studies, our research has suggested that humoral immune parameters are considered to be valuable to understand postoperative immune states of patients, and administration of PSK be effective as an immunomodulator.

Adult↗
PubMed1982

[A rare case of HGH producing adenoma in direct contact with an IC medial type aneurysm].

A rare case of HGH producing pituitary adenoma which was in direct contact with right IC medial type aneurysm was reported. A 60-year-old woman was admitted to our clinic in February, 1981, for the acromegalic features, who was treated by 5 mg/day of CB-154 since 1976 under the diagnosis of HGH secreting adenoma. The diagnosis of bitemporal hemianopsia was made in 1977, but it was disappeared at the time of admission to our clinic. A coronal section of computed tomography demonstrated a high contrasted tumor shadow in the sella turcica without definite suprasellar extension. A right carotid angiography showed the right IC-medial type aneurysm between the right posterior communicating artery and ophthalmic artery. In March, 2-81, direct neck clipping of the aneurysm and subcapsular removal of the adenoma were performed by the right frontal approach. From the operative findings it was concluded that the aneurysm was partially embedded in the adenoma tissue. Post operative course was uneventful except for the fact that the basal HGH level could not be normalized without CB-154 therapy. Many cases of association of cerebral aneurysm with pituitary adenoma were reported in the literature. However, direct contact between the tumor and the aneurysm could not be detected. This case seems the first report of the direct association of cerebral aneurysm with pituitary adenoma. Etiology for the unique combination of adenoma and aneurysm was discussed. It has been well known that the prolonged high level of serum growth hormone in acromegaly produces arteriosclerotic and degenerative changes in the arterial wall. It was speculated that the formation of the aneurysm in our case particular was due to the combination effects of the following events. That is, the direct mechanical pressure of pituitary adenoma to the right IC arterial wall, the arteriosclerotic or degenerative change of the arterial wall due to the prolonged exposure to the high level of HGH, and finally traction effect of the adenoma to the arterial wall because of the reduction of the size of the adenoma due to CB-154 therapy, all above mentioned influences were considered to contribute to the aneurysm formation. Finally, the preoperative bilateral carotid angiography is not unnecessary to the operative planning of the pituitary adenoma, especially when the adenoma was HGH producing one.

Adenoma↗
PubMed1980

Hemoglobin-haptoglobin receptor in rat liver plasma membrane.

The presence of a receptor specific for the hemoglobin . haptoglobin complex is demonstrated in rat liver plasma membranes. Hemoglobin . haptoglobin complex, administered intravenously to rats, was cleared from the circulation at a constant rate with exclusive incorporation of the molecule into hepatocytes. This incorporation was unaffected by the simultaneous injection of asialoglycoprotein or heme . hemopexin complex. In vitro experiments with isolated liver plasma membranes indicated the absence of competitive binding of these molecules to the membrane and suggested that this receptor might recognize an altered conformation of the haptoglobin moiety of the complex resulting from the binding with hemoglobin. These observations suggest that the mechanism of recognition and binding of hemoglobin . haptoglobin complex by the receptor is different from that of the asialoglycoprotein receptor or heme . hemopexin receptor.

Animals↗
PubMed1980

[Studies on the suppressor cells acting on the mitogen-induced blastogenesis in tumor-bearing hosts. I. Suppressor cells in the spleens and peripheral blood of tumor-bearing rats (author's transl)].

When Walker carcinosarcoma 256 cells were transplanted to the inbred Fischer male rats, the responses of spleen cells to Concanavalin A (Con A) or Phytohemagglutinin (PHA) stimulation were gradually reduced as tumor grew up. However, these responses were significantly restored in most cases when the phagocytic or adherent cells were removed from spleen cell suspensions by treating them with a carbonyl iron-magnetic procedure or the passing through a Sephadex G-10 column. Similarly, the decreased responses of peripheral blood mononuclear cells from tumor-bearing rats to Con A stimulation were also restored by removal of the adherent cells with a Sephadex G-10 column. These results strongly suggest a possibility that the suppressor cells, probably the monocyte-macrophage series, were contained in both spleen cells and peripheral blood mononuclear cells and these cells exert their suppressive effect on the mitogen-induced lymphocyte transformation.

Animals↗
PubMed1980

[Studies on the suppressor cells acting on the mitogen induced blastogenesis in tumor-bearing hosts. II. Suppressor cells in the peripheral blood of patients with malignant bone and soft tissue sarcoma (author's transl)].

The responses to Concanavalin A (Con A) of peripheral blood mononuclear cells were studied in 22 patients with malignant bone and soft tissue sarcoma. The decreases of response were shown in many cases depending on their clinical stages. The responses were increased by removal of the adherent cells from mononuclear cells by passing through a Sephadex G-10 column in 7 of 13 cases tested. The enhancement of responses to Con A was also found when indomethacin, a inhibitor of prostaglandin biosynthesis, was added to mononuclear cell suspensions. However, no additional increase of the responses was found when indomethacin was supplemented to the adherent cell-depleted mononuclear cell suspensions. Since it has been previously shown that prostaglandin was primarily produced from macrophages, the author concluded that the suppressor cells affecting on the lymphocyte transformation probably belong to monocyte-macrophage series and their suppressive effect may be mediated by prostaglandin. In the normal control, the responses of mononuclear cells to Con A stimulation were rather reduced by removal of the adherent cells, suggesting that these suppressor cells were contained only in mononuclear cells of the tumor-bearing hosts.

Bone Neoplasms↗