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Biomedical subjects

M Takami

Publications and source records attributed to M Takami.

At least 91 records · Page 5Linked to original sources
PubMed1995

Possible role of thromboxane A2 in hyperresponsiveness of isolated rat lung tissue in a Sephadex-induced eosinophilia model.

Antigen-stimulated contraction and release of chemical mediators were examined in saline- or Sephadex-treated rat lung parenchymal strips. Sephadex treatment caused eosinophilia in the blood and the lung tissue. Antigen challenge of the isolated parenchymal strips in Sephadex-treated rat was followed by passive sensitization, resulted in an augmented contraction and elevated releases of thromboxane (TX) B2 and peptide-leukotrienes (p-LTs) in bath fluid compared with those of saline-treated control. Although 5-hydroxytryptamine (5-HT) and histamine were significantly released after antigen challenge, the levels were not different between saline- and Sephadex-treated groups. DP-1904, a selective thromboxane synthetase inhibitor, and methysergide but not atropine significantly reduced the augmented contraction and inhibited the elevated TXB2 release in the Sephadex-treated group. Similar increased contraction and the elevated TXB2 release above were observed when Sephadex-treated rat lung strips were stimulated by exogenous 5-HT and LTD4. These augmented contractions were closely correlated with the increase in TXB2 level (r = 0.83; p < 0.01). In addition, contraction to U-46619, a thromboxane mimetic, was significantly greater in Sephadex-treated rat lung strips. Our results indicate that the ability of Sephadex-treated rat lung tissue to synthesize newly generated mediators such as TXA2 and p-LTs is increased, and the spasmogenic susceptibility of the lung tissue to TXA2 itself is modified by Sephadex treatment, suggesting these are due to the augmented contraction in an established hyperresponsiveness state induced by Sephadex.

15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5↗
PubMed1995

[Study on therapeutics adopted after occlusion of intraarterial reservoir in patients with liver tumor].

Over the last 5 years, we experienced thirty-nine patients with liver tumors undergoing implantation of an intraarterial reservoir through the gastroduodenal artery. Nine of the 39 patients had hepatocellular carcinomas, while the rest had metastatic liver tumors. In 32 patients, intraarterial chemotherapy via an implanted reservoir was discontinued either because of death in 20 patients with an average survival period of 11.7 months or because of occlusion of an intraarterial line in 12 patients with an average treatment period of 20.2 months. Regarding treatment modalities adopted for intraarterial therapy, transcatheter arterial embolization, surgical resection, microwave tumor coagulation, ethanol injection therapy, and a subselective intraarterial chemotherapy were performed in 3 patients with hepatocellular carcinomas. All of them survived more than 2 years after disuse of the reservoir. Out of 5 patients with metastatic liver tumors of colorectal cancer, one patient underwent additional surgical resection, two patients had no therapy who survived only two or three months, and two patients were still alive without additional therapies. Of four patients with metastatic liver tumors, 3 from breast cancer and one from leiomyosarcoma of stomach were treated with systemic chemotherapy or subselective intraarterial chemotherapy combined with radiation therapy. The average survival period of these 12 patients was 16.2 months, and 7 of them are still alive.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1995

[Long-term survival in two cases of multiple liver metastasis successfully treated with intraoperative ultrasound-guided microwave tumor coagulation (MTC)].

Since April 1990, multiple metastatic liver tumors have been treated with intraoperative ultrasound-guided microwave tumor coagulation (MTC) in combination with postoperative intraarterial chemotherapy. Twenty-four patients have been enrolled in this therapeutic modality and two of five patients treated in 1990 achieved long-term survival. In case 1, a 67-year-old woman was diagnosed as bearing gastric leiomyosarcoma with multiple liver metastasis. Total gastrectomy was performed, and six hepatic lesions were treated by MTC along with implantation of an intraarterial reservoir. Postoperative intraarterial chemotherapy was administered in a selective or subselective manner. Her survival time was 4 years and 6 months. In case 2, a 47-year-old man was diagnosed as having liver metastasis from descending colon cancer 1 year and 5 months after left hemicolectomy. When he was laparotomized for the treatment of adhesive ileus, three metastatic liver tumors were treated with MTC. Afterward, he underwent hepatic resection, intraarterial and intraportal chemotherapy. He survived for 5 years and 5 months after being diagnosed with liver metastasis. MTC is one of the useful modalities for the treatment of multiple liver metastasis.

Aged↗
PubMed1995

[Establishment of metastatic sub-clone from estrogen independent Ishikawa cells and its characterization in vitro and in vivo].

An estrogen (E) independent sub-clone (EIIL) was separated from a human endometrial carcinoma cell line, Ishikawa, by culturing the wild type under an E free condition for 350 days. The cells were then implanted into nude mice subcutaneously and tumors allowed to develop for 35 days. The primary lesions were then excised to stimulate recurrence. One animal developed recurrence with multiple distant metastases. The primary tumor and metastatic tumors from the animal were studied for ErbB-2 expression by immunohystochemical techniques or by a reverse transcription followed by polymerase chain reaction (RT-PCR). Expressions of epidermal growth factor (EGF) receptors, aromatase, nidogen, E receptors, hepatocyte growth factors (HGF) and beta-actin were also examined. The results showed that metastatic lesions expressed high levels of ErbB-2, nidogen and aromatase but unchanged levels of EGF receptors and HGF. The metastatic lesions expressed one third of the E receptors which were detected in the EIIL in vitro. These observations suggest that a decrease in ER along with increased expression of nidogen and aromatase is associated with the process of metastasis and the model appears to be of value in studying the process of the acquisition of a metastatic phenotype.

Animals↗
PubMed1995

[Estramustine phosphate, estrogen conjugated with nitrogen mustard inhibits the growth of endometrial cancer cells in vitro].

Estra-1,3,5 (10)-triene-3,17-diol (17 beta)-, 3-[bis(2-chloroethyl) carbamate] (Estramustine EM) was tested for its anticancer effect on human endometrial cancer cell lines: Ishikawa and its estrogen (E) independent sub-clone EIIL (Estrogen Independent Ishikawa Line). The results showed: (1) EM inhibited growth of both cell lines in a dose dependent manner giving ID50 for Ishikawa as 12 microM and for EIIL as 65 microM. (2) The addition of EM to the culture medium caused cell detachment and death associated with a breakdown of DNA to approximately 90 base pair fragments. (3) Reverse transcription-polymerase chain reaction to examine expressions of c-erbB-2, nidogen and fas showed that EM completely abolished fas expression and resulted in a 40% decrease in nidogen expression in Ishikawa but not in EIIL. No change was seen in c-erbB-2 expression. The present data indicate that the E component of EM does not stimulate the growth of Ishikawa or EIIL. Since the growth of both cell lines was inhibited but apparently in an E receptor (ER) dependent manner, EM may be of value in an adjuvant therapy for endometrial cancer, especially an ER positive one.

Adenocarcinoma↗
PubMed1995

[A case of pulmonary metastasis from colon cancer successfully treated with high-dose 5'-DFUR].

We report a case, showing a complete response to high-dose 5'-DFUR, with pulmonary metastasis from colon cancer. A 52-year-old male patient underwent right hemicolectomy for ascending colon cancer in August, 1991. A recurrence of colon cancer developed in the left lower lung 16 months after surgery, and 5'-DFUR was administered at a dose of 1,600 mg/body/day. The pulmonary metastatic lesion was undetectable on chest X-ray film 9 weeks after the start of this therapy. The dose of 5'-DFUR was then reduced to 600 mg/body/day. Although this condition was maintained for 22 weeks, chest X-ray film again showed the metastatic lesion at the same site in the lung as before. The pulmonary metastasis was resected completely in April, 1994. This is suggested to be an effective therapy for pulmonary metastasis from colon cancer.

Adenocarcinoma↗
PubMed1994

Hydrophobic blue pigment formation from phosphatidylgenipin.

Phosphatidylgenipin, synthesized via the transphosphatidylation reaction of 1,2-dipalmitoyl-3-sn-phosphatidylcholine to genipin by phospholipase D, was found to react with L-phenylalanine in chloroform and gave a clear blue solution. This blue solution was also formed in following organic solvents: ethanol, ethyl acetate, diethyl ether, benzene, and hexane. However, genipin and L-phenylalanine did not give any colored product under the same conditions. The blue pigment resulted from phosphatidylgenipin and L-phenylalanine showed lambda max at 615 nm in chloroform, and had a similar blue color to an aqueous solution of the natural blue pigment "gardenia blue." This is an example for the preparation of a hydrophobic pigment from a phosphatidyl derivative of a water-soluble compound.

1,2-Dipalmitoylphosphatidylcholine↗
PubMed1994

[Microwave tumor coagulation (MTC) in liver tumor: indication and percutaneous approach].

Indications for microwave tumor coagulation (MTC) and percutaneous approach in liver tumor were investigated. The study population comprised 26 patients with unresectable liver tumor (4 with hepatocellular carcinoma, 22 with metastatic liver tumor) who underwent MTC at our department after April 1990. Concomitant therapies were alcohol injection in 2 patients, hepatectomy in 12 and selective arterial chemotherapy in 20. Percutaneous MTC was performed on 2 patients with a single lesion under general anesthesia. Following tip coagulation electrode penetration under echo guidance, the lesion was thermally coagulated at 60W. To establish indications for MTC by the effect of thermal coagulation, survival periods were compared by underlying disease, number of masses coagulated, and maximum tumor size, in 23 patients who had undergone MTC at least 1 year previously. Thirteen of these 23 survived for 1 year or longer, including all 3 with hepatocellular carcinoma, 3 with breast cancer, 2 with leiomyosarcoma (gastric, small intestine), 4 of the 10 with colon cancer and 1 of the 2 with pancreatic cancer. According to evaluation of the degree of coagulation, complete coagulation was obtained in 11 of 23, all of whom had at most 6 tumor masses (of up to 3 cm in diameter) coagulated, and 9 of whom survived for 1 year or longer. Percutaneous MTC, of low invasiveness, proved useful as a tool of regional cancer therapy.

Breast Neoplasms↗
PubMed1994

[Cyclooxygenase in ooplasm is essential for early embryonal differentiation but not for successful fertilization in the mouse].

It has been known that inhibition of prostaglandin (PG) synthesis interferes with successful implantation. The present study dealt with the chronology in cyclooxygenase (COX) expression and the effects of inhibition in mouse oocytes in the perifertilization period. COX protein was detected in both oocytes and fertilized eggs whereas the mRNA was not found by a high sensitivity RT-PCR only in blastocysts. Oocytes which were sham operated or micro-injected with preabsorbed anti-COX monoclonal antibody (hPES01) or non-immune IgG were fertilized and developed to two-cell embryos, whereas hPES01 injected oocytes were fertilized but failed to differentiate (p < 0.05 chi 2-analysis). This inhibitory effect on early embryonal development of COX inhibition was also seen in indomethacin pre-cultured oocytes which were successfully fertilized but failed to develop further. We concluded that the COX gene which is one of the immediate early genes is not transcribed in the perifertilization period but the enzyme which remains in the cytoplasm and the presence of intact COX before fertilization are essential for early embryonal differentiation.

Animals↗
PubMed1993

Catabolism of heme moiety of hemoglobin.haptoglobin in rat liver cells in vivo.

After intravenous administration of [3H-heme,14C-globin]hemoglobin.haptoglobin or [59Fe-heme]hemoglobin.haptoglobin to rats, the radioactive materials extracted from the liver homogenate or its subcellular fractions were subjected to a gel filtration column. In addition to the 82-kDa component, we found three metabolites of heme moiety of the complex in the subcellular fractions. Intact hemoglobin.haptoglobin complex with 3H, 14C, and 59Fe radioactivities, an 82-kDa component with 3H, 14C, and 59Fe radioactivities, a 40-kDa component with 3H and 59Fe radioactivities, a lower molecular weight component with 3H and 59Fe radioactivities, and a component with 3H and 59Fe radioactivities bound to the microsome, which are referred to as peaks A, B, C, D, and E, respectively. Using a differential centrifugation technique, most of peak B was found in the mitochondria-lysosomal fraction. Peaks C and D were in the 82,500 x g supernatant fraction, while peak D was also found in the mitochondria-lysosomal fraction. The molecular weight of peak C was approximately 40 kDa, and the 3H radioactivity of peak C was eluted at the same fraction as glutathione S-transferases using both gel filtration and ion exchange chromatography. Peak E that was solubilized from the microsomes was found at the microsomal heme protein fraction on gel filtration chromatogram. Some of the 3H radioactivity in the microsomes was partially co-purified with cytochrome b5 fraction. These results indicate that there are at least four metabolites of heme moiety of hemoglobin.haptoglobin complex in rat liver cells during its catabolism and suggest that some of the heme derived from catabolized hemoglobin.haptoglobin complex binds to glutathione S-transferases in the cytosol and is incorporated into apoheme proteins in the microsomes or at least apocytochrome b5.

Animals↗