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Biomedical subjects

M Tajima

Publications and source records attributed to M Tajima.

At least 343 records · Page 19Linked to original sources
PubMed1969

Electron microscopy of equine infectious anemia virus.

Equine infectious anemia (EIA) virus was observed in thin sections of infected cultured horse leukocytes by electron microscopy. The virus particles had a spherical shape and were between 80 and 120 nm in diameter. Most of them contained an electron-dense nucleoid 40 to 60 nm in diameter. They were observed to form by a process of budding from the plasma membrane and appeared to have thin surface projections. The particles described were not detected in uninfected cultured cells, and their appearance could be prevented by adding EIA immune serum to the inoculum. The implications of these findings in the classification of EIA virus are discussed.

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PubMedDate not supplied

Variations in effectiveness of antigen retrieval pretreatments for diagnostic immunohistochemistry.

Enzymatic and non-enzymatic pretreatments for antigen retrieval (AR) on formalin-fixed, paraffin-embedded, deparaffinised sections were compared and optimised by immunostaining of tissues infected with 13 different pathogens. Enzymatic pretreatment was achieved by incubating slides in 0.25 per cent trypsin (TR) and non-enzymatic pretreatments by heating them in a microwave oven (MWO) or an autoclave (AC). Three different buffers of different pH and molarity including phosphate buffer (PB), citrate buffer (CB) and acetate buffer (AB) were used as AR solutions. All of them were effective regardless of molarity, for the MWO with a processing time of 20 minutes, and the AC with a processing time of 15 minutes. The detectability of many viral antigens was significantly improved by MWO or TR pretreatment, but that of most bacterial antigens was not improved and that of some viral antigens was decreased by TR pretreatment. These results suggest that AR pretreatment is a prerequisite procedure for immunohistochemical detection of many infectious agents but the optimal pretreatment has to be individualised for each agent.

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PubMedDate not supplied

A comparative study on anti-inflammatory activities of the enantiomers, shikonin and alkannin.

A pharmacological comparison between the enantiomers, shikonin (R) and alkannin (S), was made with regard to their inhibitory effects on the increased capillary permeability and thermal edema in rats, using phenylbutazone as a positive control in both models. The results of experiments have shown that there is no significant difference in the anti-inflammatory activity between the two compounds.

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