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Biomedical subjects

M T Ryan

Publications and source records attributed to M T Ryan.

53 records · Page 3Linked to original sources
PubMed1994

Isolation of a cDNA clone specifying rat chaperonin 10, a stress-inducible mitochondrial matrix protein synthesised without a cleavable presequence.

We have isolated a cDNA clone encoding chaperonin 10 from rat liver. The cDNA specifies a protein of 102 amino acids which, when transcribed and translated in vitro, yields a single basic product (pI > 9) that co-migrates exactly with the heat shock inducible cpn10 of rat hepatoma cells during 2D gel-electrophoresis. It is concluded that cpn10, unlike the majority of nuclear-encoded proteins of the mitochondrial matrix, is synthesised without a cleavable targeting signal and that, following removal of the initiating methionine, it becomes acetylated prior to mitochondrial import. Incubation of 3H- or 35S-labelled cpn10 with mitochondria confirms these conclusions and shows that cpn10 is imported into mitochondria in an energy-dependent process which is inhibited by the presence of 2,4-dinitrophenol.

Amino Acid Sequence↗
PubMed1989

Providing shelter.

1. Individuals with chronic mental illness are a diverse group requiring a variety of housing options to live successfully in the community. 2. Assistance in obtaining safe, affordable housing must be part of a comprehensive treatment plan for individuals with chronic mental illness if homelessness is to be avoided. 3. Nurses are caring for the homeless in many capacities, including staff and volunteer positions. 4. Single room occupancy housing provided by a supportive staff is an effective alternative to institutionalization for assisting individuals with chronic mental illness to live in the community.

Adult↗
PubMed1987

The impact of an isotopic effect on the interpretation of bioassay data for Pu.

A review of the aggregate recoil phenomenon and its potential influence on transfer rates of 238PuO2 and 239PuO2 across the human alveolar wall indicated a significant difference between these two isotopic compounds. Transfer rates were determined in in-vitro experiments and the ratio of the transfer rates for 238Pu to 239Pu was approximately 140 (Ry82). Calculations were performed to assess the importance of this finding on the internal dosimetry of insoluble 238Pu, using methods and models recommended by the International Commission on Radiological Protection (ICRP). It was found that the committed dose equivalent per unit intake was not influenced by the transfer rate from the pulmonary compartment to blood.

Administration, Inhalation↗
PubMed1978

Testosterone-induced proton release and inhibition of tryptic hydrolysis of albumin.

Further investigation of the effect of bound testosterone on the susceptibility of human serum albumin to tryptic hydrolysis has revealed a stimulation of hydrolysis at low binding levels, becoming an inhibition at higher binding levels. The inhibition, but not the stimulation, is associated with a two-stage steroid-induced proton release phenomenon which may involve the cysteine (SH) group and tyrosine residues. Proton release is also induced by Ca2+ and ethanol. The nature of the effect and the influence of Ca2+ suggests that stimulation of hydrolysis is associated with the presence of one molecule of testosterone and inhibition with the presence of 2-5 molecules at a set of sites which are thermodynamically equivalent.

Calcium↗
PubMed1976

The paradoxical effect of fatty acid on steroid-albumin interaction.

Careful investigation of the influence of palmitic and lauric acid on the interaction of progesterone and testosterone with several batches of untreated and defatted bovine and human serum albumins have revealed that, by contrast with published data for studies with progesterone as well as nonsteroid ligands, there is a surprising stimulation rather than inhibition of binding, albeit with a reduction of the apparent number of binding sites in almost all instances. Furthermore, fatty acid tends to minimize or eliminate the well-known differences in affinity between bovine and human albumin for interactions with these two steroids. The values for binding affinity in the interaction of testosterone with these batches of human serum albumin are significantly higher than those previously published by us and other authors and the value for progesterone-bovine albumin interaction is not in accordance with the "polarity rule". Studies of these same interactions by ultraviolet difference spectroscopy give further evidence of the augmentation in binding but, in the case of defatted bovine albumin only, the aromatic difference troughs are indicative of tyrosine perturbation whereas refatted bovine albumin, defatted and refatted human albumin manifest tryptophan perturbation. Quantitative correlation of perturbation with level of bound steroid suggests that fatty acid alters the ratio (possibly hydrogen-bonded to non hydrogen-bonded) of two forms of bound steroid. There is also further evidence that the binding sites for testosterone and progesterone are not identical.

Binding Sites↗