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Biomedical subjects

M T Rossi

Publications and source records attributed to M T Rossi.

At least 19 recordsLinked to original sources
PubMed1990

In vitro non-enzymatic glycation and formation of browning products in the bovine lens alpha-crystallin.

Calf lens LMW alpha-crystallin was glycated by incubating with various sugars (glucose, glucose-6-P and ribose) for 21 days. All sugars induced disulfide formation, but ribose also produced higher molecular weight cross-linked species. The ribocated protein turned yellow in color and had a strong blue fluorescence (Ex/Em = 370/450 nm) typical for a browning product. The chromophore of the browning product showed a new circular dichroism (CD) band at 320-330 nm. Conformational study indicated that the browning reaction destablized protein and may play a significant role in protein aggregation and insolubilization.

Animals↗
PubMed1989

Limiting dilution analysis for detection of residual leukemic cells after bone marrow combined decontamination with mafosfamide followed by merocyanine-540-mediated photosensitization.

Human acute myelogenous or lymphoblastic leukemia cells of the K-562 and CCRF-SB lines were mixed with an excess of normal human bone marrow cells to simulate a leukemia remission marrow. The cell mixtures were then incubated in vitro with mafosfamide (AZ) followed by the photoreactive dye merocyanine-540 (MC). Treated cells (1 x 10(4] were seeded in microwell plates, and increasing numbers of the line used to contaminate the normal marrow were added. Treatment with AZ alone produced total elimination (i.e., 6 logs) of CCRF-SB cells, while addition of merocyanine-540 increased the cloning efficiency from 22% to 24.4%. After treatment of the K-562-contaminated cell mixtures with AZ, nearly 1.6 logs of K-562 acute myelogenous blasts were still present, whereas AZ purging followed by MC-mediated photosensitization resulted in 100% elimination of clonogenic cells. Moreover, the combined treatment caused an increase of the cloning efficiency from 37.3% to 62%, clearly indicating that cleansing by the two agents combined was more effective than treatment with one agent alone.

Antineoplastic Agents↗
PubMed1982

NMR determination of isosorbide dinitrate and beta-adrenergic blocking agents in tablets.

An NMR spectroscopic method for the determination of isosorbide dinitrate, alone or together with alprenolol or propranolol, is described. Spectra are determined in dimethyl sulfoxide-d6 containing maleic acid or 1,4-dinitrobenzene as internal standards. Both synthetic mixtures and commercial formulations were assayed, and the results were compared using compendial procedures.

Adrenergic beta-Antagonists↗
PubMed1979

[Determination of taste threshold].

A linear inverse relation links the HCl solution molarity to both the absolute HCl solution intake, and the ratio of the HCl solution to the total fluid intake. The last algebraic relation allows the taste threshold to be estimated.

Animals↗
PubMed1979

[Peripheral and central factors which modify the taste threshold].

Several anatomical, physiological and behavioral factors have part in changing the values of the detection threshold, among which the reflexly secreted oral fluid. The HCl molar concentration appears to be a hyperbolic function of preference (= taste solution/water ratio); the empirical formula of the function makes the HCl molar concentration to be known, at which the same voll. of water and taste solution are drunk, i.e. the taste threshold.

Animals↗
PubMed1979

[In vitro studies on megakaryocytes.-I-].

Normal rat's recent bone marrow has been suspended in an isoionic, oxygenated, warmed solution; platelets production by megakaryocytes has never been verified.

Animals↗