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Biomedical subjects

M T Moss

Publications and source records attributed to M T Moss.

At least 37 records · Page 2Linked to original sources
PubMed1991

Specific detection of Mycobacterium paratuberculosis by DNA hybridisation with a fragment of the insertion element IS900.

This paper describes the evaluation of a newly developed DNA probe for Mycobacterium paratuberculosis. DNA probe PCR278 is a 278 bp fragment obtained by polymerase chain reaction (PCR) amplification of the 5'-region of IS900, an insertion element contained in the genome of M paratuberculosis. This DNA probe can specifically distinguish M paratuberculosis from a wide range of other organisms, including members of the M avium-M intracellulare complex. When used in conjunction with the PCR amplification technique DNA probe PCR278 could detect as little as 10 fg (equivalent to two genomes) starting material of M paratuberculosis genomic DNA. Use of PCR amplification assays based on IS900, for the detection of M paratuberculosis, and homologous IS elements found in disease isolates of M avium should greatly help our understanding of the role of these organisms in Crohn's disease and other chronic inflammatory disorders.

Base Sequence↗
PubMed1989

Sequence and characteristics of IS900, an insertion element identified in a human Crohn's disease isolate of Mycobacterium paratuberculosis.

The complete sequence of an insertion element IS900 in Mycobacterium paratuberculosis is reported. This is the first characterised example of a mycobacterial insertion element. IS900 consists of 1451bp of which 66% is G + C. It lacks terminal inverted and direct repeats, characteristic of Escherichia coli insertion elements but shows a degree of target sequence specificity. A single open reading frame (ORF 1197) coding for 399 amino acids is predicted. This amino acid sequence, and to a lesser extent the nucleotide sequence, show significant homologies to IS110, an insertion element of Streptomyces coelicolor A3(2). It is proposed that IS900, IS110, and similar insertion elements recently identified in disease isolates of Mycobacterium avium are members of a phylogenetically related family. IS900 will provide highly specific markers for the precise identification of Mycobacterium paratuberculosis, useful in defining its relationship to animal and human diseases.

Amino Acid Sequence↗
PubMed1989

DNA probes demonstrate a single highly conserved strain of Mycobacterium avium infecting AIDS patients.

Strains of the Mycobacterium avium intracellulare complex (MAIC) have become important colonisers of patients with acquired immunodeficiency syndrome (AIDS). Restriction fragment length polymorphisms were used to study the DNA from 88 MAIC isolates, including 51 derived from 47 AIDS patients. MAIC isolates from 33 of 45 AIDS patients were identical at the molecular level and distinct from the mycobacteria isolated from the stools of healthy subjects. The study also showed that serotyping correlates poorly with the genetic identity of these organisms. Mycobacterium paratuberculosis, which has been implicated in Crohn's disease, was not identified in any of the cultures studied.

Acquired Immunodeficiency Syndrome↗
PubMed1989

Insertion elements in mycobacteria.

We have isolated and characterised a repetitive element from the genome of Mycobacterium paratuberculosis. This repetitive element shows many features characteristic of a bacterial insertion element.

Cloning, Molecular↗
PubMed1986

Cloning and expression of Mycobacterium bovis BCG DNA in "Streptomyces lividans".

The ability of "Streptomyces lividans" to use the expression signals of genes from Mycobacterium bovis BCG was tested in vivo by using gene fusions. Random DNA fragments from M. bovis BCG were inserted into promoter-probe plasmids in Escherichia coli and in "S. lividans." Comparison with promoter activity detected with random DNA fragments from the respective hosts suggested that "S. lividans" efficiently utilizes a high proportion of mycobacterial promoters, whereas a smaller fraction are expressed, and expressed more weakly, in E. coli. M. bovis BCG DNA fragments were also inserted into the specially constructed translational fusion vector (pIJ688) in "S. lividans." pIJ688 contains the kanamycin phosphotransferase gene (neo) from transposon Tn5, truncated at its amino terminus, as the indicator. The results suggested that "S. lividans" uses M. bovis BCG translational signals almost as efficiently as its own signals. Moreover, several hybrid proteins with an M. bovis BCG-derived amino terminus seemed to be reasonably stable in "S. lividans." These experiments indicate that "S. lividans" may be a suitable host for the expression of Mycobacterium leprae and Mycobacterium tuberculosis genes from their own signals. This is a precondition for the expression of entire biosynthetic pathways, which could be valuable in the production of diagnostic and therapeutic agents. The vectors may also have wider applications for the analysis of gene expression in Streptomyces.

Cloning, Molecular↗
PubMed1976

Out of the frying pan into the fire?

During the last 10 years or so, many departments of therapeutic radiology have lost their medical and fiscal independence by administrative incorporation into "departments of oncology." The author feels that the aims of oncology centers, viz., consolidation of cancer-related activities, promotion of the image of a functioning cancer center, and the multidiscipline care of cancer patients, do not justify the radiotherapist's loss of identity within such a complex.

Hospital Departments↗
PubMedDate not supplied

Principles, values, and ethics set the stage for managed care nursing.

Principles, values, and ethics constitute an ethical system that provides nurses a context in which to make ethical decisions. As the managed care environment generates new ethical issues and intensifies already existing ones, ethical education and decision-making skills become even more critical to nursing professionals, as well as physicians and hospital administrators.

Decision Making, Organizational↗
PubMedDate not supplied

Operating room budget factors: a pocket guide to OR finance.

This pocket guide will provide step-by-step directions how to manage departmental finances and how to compete in a managed care environment using cost-based and break-even analysis. Several suggestions for enhancing revenue generation will also be provided.

Budgets↗
PubMedDate not supplied

Service integration in the reform era.

Fifty years of health care reform have centered around "fixing up" existing operational processes by examining the tasks attached to those processes. Today, restructuring seeks to identify the core processes of hospitals, such as patient care, and reorganize around those processes. Reorganization is painful, but also exhilarating. If hospitals are to use this exhilaration as a source of growth in a new economic climate, management must redefine itself and its relationship to staff.

Health Care Reform↗
PubMedDate not supplied

Outcomes management in perioperative services.

Unit-based outcomes management addresses DRG-specific patient populations, making established services more economically efficient within critically monitored populations. Perioperative outcomes management balances quality of care and costs.

Clinical Protocols↗
PubMedDate not supplied

An interview with Mae Taylor Moss. Interview by Connie R. Curran.

Mae Taylor Moss, MSN, RN, the recipient of the 1992 Nursing Economic$ Excellence Award, is assistant vice president of perioperative services, St. Luke's Episcopal Hospital, Houston, TX. In this interview, Ms. Taylor Moss discusses the challenges facing her department and her strategies for decreasing costs while increasing revenues and motivating staff.

Financial Management, Hospital↗
PubMedDate not supplied

Practical budgeting for the operating room administrator.

To meet the challenge of an annual budget controlled by revenue projections and cost-containment initiatives, an OR administration reviewed all expenditures, planned new initiatives, evaluated service expansion, and created an environment to encourage new products and services while maintaining quality. This revenue enhancement project maintained the institution's market share and increased revenue.

Budgets↗