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Biomedical subjects

M T Blanco

Publications and source records attributed to M T Blanco.

25 records · Page 2Linked to original sources
PubMed1989

Phagocytic activity of polymorphonuclear leukocytes on Escherichia coli previously exposed to metronidazole.

A study was made of the action of different concentrations of metronidazole of the viability of Escherichia coli under aerobic and anaerobic conditions. The viability of E. coli was reduced by 60 to 99% after 24 hours of anaerobic incubation, according to the concentration of metronidazole tested. In addition, there were significant morphological changes in the bacteria. Exposure of antibiotic-induced filaments of E. coli LP 136 to phagocytosis caused the cfu/ml value to drop by 60% after 120 minutes. Under identical conditions, using the mutant strain E. coli RYC 819, which did not become filamented by metronidazole although it did present similar ultrastructural changes, this reduction reached 83%. These results may explain the therapeutic success of metronidazole in polymicrobial infections.

Adult↗
PubMed1987

The in-vitro activity of metronidazole against Enterobacteriaceae alone and in mixed cultures with Bacteroides fragilis.

The in-vitro antimicrobial action of therapeutic concentrations of metronidazole against Bacteroides fragilis and six different strains of Enterobacteriaceae in pure and mixed cultures have been studied. Under anaerobic conditions, metronidazole suppressed the growth of pure cultures of the Enterobacteriaceae. A reduction in the viable counts from 10(9) cfu/ml, in the 24 h controls, to 10(8), 10(7) and 10(5) cfu/ml in the presence of 10, 50 and 100 mg/l of metronidazole respectively, was observed. These concentrations of drug produced a marked bactericidal effect against B. fragilis, as expected. The antimicrobial activity of metronidazole on mixed cultures of B. fragilis and each one of the Enterobacteriaceae studied was greater against both micro-organisms than the corresponding effect on their respective pure cultures, under the same experimental conditions.

Bacteroides fragilis↗
PubMed1986

Effect of growth rate and aeration on the production of microcin by Escherichia coli growing in continuous culture.

The production of microcin 15, a low molecular weight antibiotic, resistant to proteases and antagonized by methionine, was measured in batch and continuous cultures of E. coli LP 136. The highest yield per cell was obtained in conditions (anaerobiosis, low glucose concentration, low growth rate) similar to those found in the intestinal tract. It is postulated that microcin production may be an important factor in growth competition between enterobacteria in the intestinal tract.

Aerobiosis↗