Search PubMed⌕ Search

Biomedical subjects

M Szpakowski

Publications and source records attributed to M Szpakowski.

At least 55 records · Page 3Linked to original sources
PubMed1994

Antinuclear antibodies in women with recurrent pregnancy wastage and their prognostic value for immunotherapy.

Autoimmune diseases are suspected of causing some cases of recurrent pregnancy loss. We sought serological evidence of such diseases in women with recurrent pregnancy wastage. Antinuclear antibodies were studied administrating indirect immunofluorescence test using HEP-2 cells as targets. We compared the frequency of a low-level antinuclear antibody titers in the 68 women with a history of unexplained recurrent pregnancy losses (group A) to that in 35 with explained repeated pregnancy losses (group B) and 44 healthy pregnant (group C), and 36 healthy nonpregnant women (group D). The frequency of positive ANA tests at a titer of 1:40 or higher was 51.5% in group A, 34.3% in group B, 6.8% in group C and 5.6% in group D. No negative influence on pregnancy outcome was found in case of occurrence of ANA in group A where women were treated with husband's lymphocyte immunisation. The success rate was 78.6% in patients with positive ANA and 62.5% in patients with negative ANA. This study demonstrates a high prevalence of low-titer antinuclear antibody-positive serum in patients with both explained and unexplained pregnancy losses; however, the significance of these findings remains unclear.

Abortion, Habitual↗
PubMed1994

Essential edema-proteinuria-hypertension (EPH) gestosis and gestosis superimposing pre-existing renal disease: comparison of cellular immunity parameters.

The paper is aimed at evaluation of certain indices of cellular immunity in pregnant women with gestosis superimposing pre-existing renal diseases and comparing them with the immunological condition of women with essential gestosis. T lymphocyte subpopulations and lymphocyte proliferative responses to mitogens (phytohemagglutinin--PHA, concanavalin A--Con A and pokeweed mitogen--PWM) in the fetal calf serum and autological sera were studied. The groups of examined pregnant women in the 3rd trimester did not vary with regard to gestation age, calendar age and to the severity of edema (E), proteinuria (P) and hypertension (H) gestosis symptoms. It has been found that equal changes occur in both groups of women, regarding the examined indices of cellular immunity, when referred to normal pregnant women. In the gestotic women a decreased absolute and percentage content of CD8+ T cells and increased percentage of CD3+ and CD4+ lymphocytes were found in comparison with the normal pregnant women, which led to an almost 2.5-fold increase of CD4+/CD8+ ratio. The sera of gestotic women, in comparison with the healthy pregnant group, strongly increased the proliferation of Con A and PWM-induced lymphocytes. It may then be assumed that the observed immunological changes do not coincide with the primary causes of gestosis. We suggest that immunological factors largely contribute to the development of essential gestosis and gestosis superimposing pre-existing renal disease.

Adult↗
PubMed1992

[Certain factors of cellular immunity in clinical evaluation of women with EPH gestosis symptoms].

The applicability of clinical examination was studied in women with symptoms of medium-advanced or advanced primary gestosis of subpopulation of T lymphocytes (CD3+, CD4+, CD8+) in the peripheral blood. Also studied was the usefulness of the proliferative activity of lymphocytes in in vitro cultures: spontaneous and mitogenic (PHA, ConA, PWM)--in the environment of fetal calf serum (FCS) and autologous sera: heat-inactivated and heat-non-inactivated. It has observed that the percentages of particular T Lymphocyte subpopulations and the proportion of CD4+ lymphocytes to CD8+ lymphocytes in the peripheral blood and the mitogenic activity of the heat-non in activated autologous serum in the test of lymphocyte spontaneous blastic transformation reveal a correlation with an enhancement of particular clinical symptoms of EPH gestosis and with the birth condition of newborns.

Adult↗
PubMed1992

[Spontaneous proliferative activity of lymphocytes in normal and EPH gestosis complicated pregnancy].

The spontaneous proliferative activity of lymphocytes and CD4+/CD8+ ratio has been estimated in normal and EPH gestosis-affected pregnancy; also the effects of autologous and allogenic sera on this lymphocyte activity was evaluated. Although in normal pregnancy there was a very low CD4+/CD8+ ratio, the spontaneous proliferative activity of lymphocytes and the effects of autologous sera exerted thereupon were the same in either healthy pregnant women or in non-pregnant controls, what indicates a normal cellular immunity in physiological pregnancy. In gestosis an increased proliferative activity of lymphocytes and very high CD4+/CD8+ ratio was observed, when referred to non-pregnant controls. The serum of normal pregnant women did not reveal any immunosuppressive features against the activity of both autologous lymphocytes and gestosis--affected pregnancy, whereas it clearly inhibited the lymphocyte proliferation in non-pregnant controls. A different model of the autologous sera effects on the lymphocyte activity in gestosis women--when compared with the other examined women--is an evidence for altered immuno-modulating parameters of the serum in question.

CD4-CD8 Ratio↗
PubMed1992

[Subpopulations of t lymphocytes and lymphocyte proliferative activity in normal pregnancy].

T lymphocyte subpopulations (CD3+, CD4+, CD8+) and the lymphocyte proliferative responses to mitogens (PHA, Con A, PWM), in the environment of fetal calf serum (FCS) were examined in 16 normal primigravidas in the third trimester of pregnancy and in 15 healthy nonpregnant women. In normal pregnant women significantly lower absolute and percentage numbers of CD3+ and CD4+ T cells and almost twice reduced CD4+/CD8+ ratio were found, in comparison with nonpregnant subjects. Despite the shifts among particular T lymphocyte subsets in the peripheral blood, non disorders were found in the lymphocyte proliferative responses to mitogens in normal pregnancy.

Adult↗
PubMed1992

[Immunomodulating properties of sera in normal pregnant women].

The purpose of this study was to analyze the immunosuppressive properties of sera of normal pregnant women. Effects of pregnancy sera on the proliferation of mitogen (PHA, Con A, PWM)-induced lymphocytes were examined in healthy primiparas. The sera of normal pregnant women inhibited the proliferation of PHA- and Con A- induced both autologous and allogenic lymphocytes (obtained from nonpregnant and pre-eclamptic women), while remaining neutral towards the proliferation of PWM- induced lymphocytes in comparison with fetal calf serum. The basic immunomodulatory activity of the sera in pregnant women was contained in the heat-stable fraction.

Adult↗
PubMed1985

Chromosome studies in 3665 consecutive newborn children.

Results of chromosomal studies done on 3665 newborn children from central Poland have been described. As against a global incidence of 1:1022 for trisomy G and 1:1308 for the balanced translocation D/D in infants, the respective figures for the Polish infants were, 1:610 and 1:1221. Likewise, against the global incidence of 1:823 for the karyotype 47, XXY and of 1:1072 for the karyotype 47, XYY in infants, the respective incidence in the Polish infants was 1:626 and 1:939. The study has demonstrated also that the frequency of occurrence of the different widths of the C-bands had a distribution close to normal. The authors have noted that the children with chromosomal abnormalities were more frequently not as well developed at birth as were children with normal karyotypes.

Adult↗