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Biomedical subjects

M Szczygiel

Publications and source records attributed to M Szczygiel.

10 recordsLinked to original sources
PubMed1999

Teratozoospermia and its effect on male fertility potential.

Teratozoospermia and its significance for male fertility problems is discussed. Different points of view and available information concerning the phenomenon of teratozoospermia have been presented. Ongoing debate about the influence of sperm morphology on male infertility and IVF results emphasizes the fact that when sperm morphology is evaluated using strict criteria, this parameter is thought to have an excellent predictive value and shows a significant positive correlation with successful fertilization. Selective abilities of zona pellucida to differentiate between morphologically normal and abnormal spermatozoa, the superior role of acrosomal morphology assessment in the prediction of IVF outcome, and the involvement of cell surface molecules in the adhesion of spermatozoa to oocytes are described. The usefulness of the hamster oocyte penetration assay (HOPA), which is important not only because of its predictive value of sperm function, but also because of its role as a tool for visualizing sperm chromosomes, was found to be questionable in cases of teratozoospermia. Cytogenetic findings related to teratozoospermia are controversial as classical methodology of visualizing sperm chromosomes (through HOPA) may cause selection of sperm metaphases which can be obtained only from good-penetrating spermatozoa. There are few reports presenting chromosome complements of abnormal sperm visualized with the help of micromanipulation. There is clear evidence that a variety of genetic defects can affect spermatogenesis. The possible genetic background of teratozoospermia is discussed.

Animals↗
PubMed1983

Plasma insulin and insulin kinetics in growing sheep. Influence of age and diet.

This study was carried out to clarify the nutritional control of insulin metabolism in growing sheep fed a control or an experimental diet low in crude protein and high in propionic acid used as a feed additive. Daily variations in blood insulin and the disappearance from the circulation of unlabelled injected insulin were investigated. These data were used to calculate the metabolic clearance rate of insulin and insulin secretion. At 23 kg of body weight (BW), blood insulin showed an increase in the control group at 10 a.m. (i.e. 2 h after feeding) and a large peak in the experimental group at 3 p.m. At 33-kg BW, blood insulin in both groups showed a peak at 10 a.m. and a moderate increase at 3 p.m. Mean plasma insulin throughout the day (except at 3 p.m.) rose with increasing BW. It was lower in the experimental than in the control group. Injected insulin disappeared rapidly from the circulation; its half-life was constant (13 min). In both groups, the insulin level along the disappearance curve was higher at 33-kg BW than at 23-kg BW, and insulin metabolic clearance rate decreased. Except at 3 p.m., the mean insulin secretion rate over the day was lower in the control group at 33-kg BW than at 23-kg BW. Feeding the experimental diet increased the insulin level along the disappearance curve. In young animals, it decreased insulin metabolic clearance rate and diminished mean insulin secretion rate over the day, except at 3 p.m. when the insulin secretion rate increased. In old animals, the experimental diet did not significantly change the kinetic parameters of insulin metabolism.

Aging↗
PubMed1983

Insulin binding to liver plasma membranes from growing ruminating sheep. Maintained on different diets.

The purpose of this work was to investigate insulin receptors in growing ruminant sheep given a control diet or undergoing nitrogen restriction. Live weights ranged within 30-36 kg in both groups. Before the animals were fed (i.e. at 8 a.m.) blood insulin was not significantly different in the two groups (0.39 vs 0.53 nM in experimentals and controls, respectively). The 125I-monoiodoinsulin binding to purified liver plasma membranes was studied. Membrane recovery and purity were similar in both groups. Results showed that specific 125I-iodoinsulin binding increased with time and reached a maximum value within 60-120 min. Increasing the nonlabelled insulin level inhibited 125I-iodoinsulin binding at steadystate. Among the animals from both groups, specific insulin binding decreased significantly with increased live weight. In addition, specific insulin binding lowered with increasing blood insulin. The latter relationship partly reflected the insulin binding-live weight-blood insulin relationships. The insulin binding was similar in both groups. Furthermore Scatchard analysis indicated no significant differences between apparent affinity constants and apparent binding capacities in the two groups.

Animals↗
PubMed1981

Prognostic indicators of ultimate long-term survival following advanced life support.

In an attempt to establish prognostic indicators for ultimate long-term survival following advanced life support (ALS), a two-year (1978-79) retrospective study was made of 92 survivors. The survivors were divided into two groups: the first survived admission to the intensive care unit, but subsequently died in the hospital; the second survived to ultimate discharge from the hospital. The groups were compared for sex, age, basic and advanced provider response time, scene an transport time, witnessed versus unwitnessed arrest, traumatic versus medical arrest, type of airway, presence of aspiration, initial cardiac rhythms, post-arrest vital signs, number of defibrillations, post-arrest ECG changes, essential and useful drugs used, and duration of basic and advanced life support. Of all prognostic indicators studied, duration of ALS correlated most closely with ultimate survival. This article advances the definition of ultimate survival of ALS to mean discharge of the patient from the hospital, and advocates this as the most objective evaluation of successful ALS. The article furthermore offers duration of ALS as an effective prognostic indicator of a patient's chance for ultimate survival.

Critical Care↗
PubMedDate not supplied

[Free amino acids in leukemic leukocytes].

Using the method of ion-exchange chromatography in automatic analyser the level of free amino acids was determined in peripheral blood leucocytes of 14 healthy blood donors and 36 patients with various types of leukaemia. In comparison with granulocytes the concentration of free amino acids in lymphocytes was reduced. The concentration of free amino acids in lymphocytes of chronic lymphatic leukemia was not essentially changed. The leucocytes in chronic myeloid leukemia showed a rise in the concentration of most free amino acids. In the blast cells of patients with acute myeloid the concentration of free amino acids was reduced in relation to DNA contents while in relation to proteins this concentration was raised.

Adult↗