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Biomedical subjects

M Sugi

Publications and source records attributed to M Sugi.

At least 55 records · Page 3Linked to original sources
PubMed1984

Isozymic changes in myosin of human atrial myocardium induced by overload. Immunohistochemical study using monoclonal antibodies.

An immunohistochemical study using monoclonal antibodies specific for the heavy chains of either human atrial (HC alpha) or ventricular (HC beta) myosin was performed to clarify the distribution of each isozyme in normal as well as pressure-overloaded human hearts. In normal human ventricles, all muscle fibers were stained by a monoclonal antibody (HMC14) specific for HC beta, whereas a small number of fibers reacted with a monoclonal antibody (CMA19) specific for HC alpha. In contrast, in normal human atria, almost all muscle fibers were stained by CMA19, and a relatively larger number of muscle fibers also reacted with HMC14. Furthermore, in pressure-overloaded atria, muscle fibers reactive with HMC14 were strikingly increased while those reactive with CMA19 showed a corresponding decrease. The extent of this isozymic redistribution was in good correlation with atrial pressure. These results not only confirmed the existence of isoforms of myosin heavy chain in human hearts, but also demonstrated that redistribution of iso-myosins could occur as an adaptation to pressure overload.

Animals↗
PubMed1984

Continuous culture of human melanoma cells in protein-free medium: establishment and partial characterization.

Human melanoma cells (MEC) continuously grown in protein-free RPMI-1640 medium were established. These cultured cells, designated "sf-MEC," grow well in suspension and have been successfully subcultured more than 100 passages for 30 months with complete loss of serum requirement for their growth. Although sf-MEC as well as MEC had a moderate number of mature melanosomes in the cytoplasm, the tumorigenicity in nude mice was lower in sf-MEC than in MEC. In the tumor tissue formed by sf-MEC but not by MEC, a few immature melanosomes and an increased C-type-like virus production were detected. Furthermore, conditioned medium of sf-MEC stimulated DNA synthesis in both normal and transformed cells, including MEC and sf-MEC. Addition of the conditioned medium of sf-MEC in its cell growth assay caused a twofold to eighteenfold increase in cell number as compared to that for the control.

Animals↗
PubMed1983

[Sensitivity to bleomycin of human cultured tumor cells and analysis of related factors].

Several types of human cultured tumor cells were tested for the sensitivity to BLM, an effective antitumor antibiotic for epidermoid (squamous cell) carcinomas. Three cell lines (HeLa, KB, Hepd) derived from epidermoid carcinomas were very sensitive to BLM under concentrations tested, whereas BLM-resistant HeLa cells (HeLa-BLMr), neoplastic cells derived from salivary glands (HPA, HSG), malignant fibrous histiocytoma (MFH) and melanoma (MEC) were much less sensitive to BLM than epidermoid carcinoma cell lines under the same conditions. To investigate the possible mechanism of BLM resistance, these cell lines, namely HeLa, HeLa-BLMr, HSG and MEC, were examined for i) BLM permeability into cells by using 3H-PEP which was a new derivative of BLM, ii) BLM-inactivating activity in cell extracts by bioassay for antibacterial activity with B. subtilis PCI 219 strain, and iii) DNA repair activity after UV irradiation. Consequently, as compared with BLM-sensitive HeLa cells, BLM less sensitive HeLa-BLMr, HSG and MEC cells showed 34%, 50% and 39% reduction per 10(6) cells in BLM permeability, 1.6-, 5.6- and 4.7-fold increase per mg protein in BLM inactivating activity, and 24.5-, one and 8-fold enhancement in DNA repair activity, respectively. Therefore, it was indicated that above three factors at least were involved in the BLM sensitivity of human tumor cells.

Bleomycin↗
PubMed1982

Microinjection of macromolecules into normal murine lymphocytes by means of cell fusion. II. Enhancement and suppression of mitogenic responses by microinjection of monoclonal anti-cyclic AMP into B lymphocytes.

Reproducible methods are now available for introducing protein molecules such as antibodies into normal murine lymphocytes by fusion with protein molecule-containing erythrocyte ghosts. Monoclonal antibodies against cyclic AMP were raised by hybridoma technique and packed into erythrocyte ghosts. Then, monoclonal anti-cyclic AMP containing ghosts were fused with splenic B lymphocytes by polyethylene glycol-mediated fusion at various intervals after LPS stimulation. This method made it possible for us to quantitatively microinject antibodies into B lymphocytes. Microinjection of anti-cyclic AMP antibody molecules into lymphocytes at a very early stage of LPS stimulation resulted in a marked enhancement of DNA synthetic responses as well as increased numbers of plaque-forming cells. Intracellular cyclic AMP levels were found to be markedly decreased after microinjection of monoclonal anti-cyclic AMP, suggesting that lowering the intracellular cyclic-AMP level in the B lymphocytes at an early stage of stimulation might have induced the enhanced proliferative as well as differentiative responses to LPS. Similar enhancing effects on cell proliferation were obtained when antibodies were injected 18 hr after stimulation. Microinjection of anti-cyclic AMP at 12 hr after culture, however, inhibited the DNA synthetic responses, and induction of plaque-forming cells was suppressed when anti-cyclic AMP was injected 6 hr after LPS stimulation. The present data suggest the biphasic regulatory roles of cyclic AMP at the early stage of B lymphocyte activation. This approach may be useful in identifying regulatory molecules in B lymphocyte induced by mitogenic or antigenic stimulation.

Animals↗
PubMed1981

Carcinoma of the maxillary sinus with eosinophilia. Report of a case.

A patient with carcinoma of the maxillary sinus presented with a blood eosinophilia and infiltration of eosinophilic leukocytes into the tumor tissue. Immediately after cancer therapy with intraarterial infusion of 5-fluorouracil into the superficial temporal artery, necrotomy and resection of the maxilla including radical neck dissection, the number of eosinophilic leukocytes was suddenly decreased and thereafter a transient increase in blood eosinophilic leukocyte count was observed. In this communication, a tentative mechanism for these events is suggested.

Carcinoma, Squamous Cell↗
PubMed1981

[Genetic study of ossification of the spinal ligaments -hereditary factors for ankylosing hyperostosis (author's transl)].

There has been no report concerning genetic investigation of ankylosing hyperostosis (AH). This is to report the influence of hereditary factor for AH on seventeen probands and fourty-two relatives of sixteen AH families. 1) In the AH families ossifications of the yellow ligament (OYL), posterior longitudinal ligament (OPLL), and supraspinous ligament (OSSL) were more frequently observed roentgenographically in or around the spine, than in the control group. There were 32 cases with OYL, 8 with OPLL and 29 with OSSL among the total of 59 cases. Especially OYL and OSSL were frequently seen even in such younger cases as aged thirties. 2) There were 6 families in which the sibships of probands were able to be examined. Eleven of 15 cases or more than one-half of the sibships were affected by AH in the 6 families. Fourteen out of 15 had some ossifications of the spinal ligament. 3) In this series, Hahn's groove of the vertebral body was observed in 44 out of 59 members. Hahn's groove is presumed to be a causative factor of AH, which we have already reported, and is rarely encountered in normal persons after the age of 20. These results suggest that there exists a genetic diathesis of ossification of the spinal ligaments and a tendency of heredity of ankylosing hyperostosis.

Adult↗