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Biomedical subjects

M Suda

Publications and source records attributed to M Suda.

At least 145 records · Page 8Linked to original sources
PubMed1989

Histiocytic hemophagocytosis in the bone marrow in children with sepsis and disseminated intravascular coagulation.

Two children with systemic E. coli and candidial infections developed disseminated intravascular coagulation (DIC). Bone marrow examination in both cases showed histiocytic hemophagocytosis, consistent with the diagnosis of the hemophagocytic syndrome. Histiocytic hemophagocytosis in the bone marrow, one of the markers of the activated mononuclear phagocyte system, might be common in patients with severe sepsis and DIC, especially in immunodeficiency.

Adolescent↗
PubMed1989

Malignant histiocytosis involving pancreas at initial presentation.

A 10-year-old boy with malignant histiocytosis presented with fever, hepatosplenomegaly, and diffuse pancreatic enlargement, mimicking acute pancreatitis. Although malignant histiocytosis involving pancreas at initial presentation is exceptional, this entity should be included in the differential diagnosis of acute pancreatitis, especially when hepatosplenomegaly and pancytopenia are present.

Acute Disease↗
PubMed1989

[Prognosis for the deciduous molars of juvenile dogs after root canal filling using apatite root sealer].

There are various restrictions on the root canal filling of deciduous roots after root canal treatment unlike in the case of permanent teeth, because deciduous molars undergo physiological root resorption with aging. Among the restrictions, it is most important that the root canal filling material be similarly absorbed and disappear along with the deciduous root so that it does not prevent the eruption of the permanent successors. Hardly any of the root canal filling materials currently on the market, however, are suitable for deciduous molars because some of them disappear earlier than the deciduous root without becoming indurated in the root canal, while some delay tooth replacement because they are resorbed with great difficulty, and continue strongly to stimulate the surrounding tissue of the root apex. Root canal filling materials utilizing alpha-TCP and hydroxyapatite with excellent biocompatibility have recently been put on the market by Sankin Kygyo Co. Type-III, in particular, contains only 5% iodoform, causing only very slight stimulation, and is a relatively good product from a practical standpoint, i.e., filling the root canal using a lentulo. Root canal filling was performed immediately after dental pulp extraction from the deciduous mandibular molars of juvenile dogs to determine the feasibility of filling deciduous teeth with root canal filling materials. Three dogs were then observed for sequential changes in the resorption of the deciduous roots, presence/absence of adverse effects on permanent successors, and influence on the process of eruption by means of radiographic examination every week. The results obtained were as follows: 1. The type-III apatite root sealar or Vitapex used for root canal filling was not absorbed, and there was almost no resorption of the root. Thus, the deciduous teeth on the experimental side were ejected and shed. 2. There was no significant difference in the amount of tooth germ formed between the experimental and control sides. 3. The amount of eruption tended to be delayed because of insufficient resorption of the root of the deciduous predecessor, but this tendency was considered to be due to the influence of the timing of root canal filling and individual difference. In the final analysis, no definite conclusions could be drawn.

Animals↗
PubMed1989

Effect of probenecid on oxypurines in plasma.

Probenecid decreased the plasma concentration of oxypurines (hypoxanthine and xanthine) but did not increase the renal excretion of oxypurines. However, the concentrations of hypoxanthine and nucleotides (inosine monophosphate, adenosine monophosphate, adenosine diphosphate, adenosine triphosphate, guanosine diphosphate and guanosine triphosphate) in red blood cells did not change after the administration of probenecid. In addition, the drug did not inhibit adenosine deaminase, purine nucleoside phosphorylase, hypoxanthine guanine phosphoribosyl transferase and xanthine oxidase in vitro. These results suggested that the rapid fall of plasma concentration of uric acid due to the potent uricosuric action of probenecid resulted in the fall of plasma concentration of oxypurines.

Adenosine Deaminase↗
PubMed1989

Virus-associated hemophagocytic syndrome: the diagnostic usefulness of immature histiocytes with benign features in the bone marrow.

A 2-year-old girl developed fever, hepatosplenomegaly, jaundice, lymphadenopathy and pancytopenia. Bone marrow examination revealed increased immature histiocytes (5.5%) and mature histiocytes with hemophagocytosis. All the abnormalities were normalized in one month without any chemotherapy. It was suggested that the presence of immature histiocytes with benign features, even if their number exceeds that of mature histiocytes, does not favor the diagnosis of malignant histiocytosis.

Bone Marrow↗
PubMed1989

The effect of phenobarbital on human plasma lipids.

The effect of phenobarbital to increase the activity of hepatic microsomal enzyme system, resulting in an increase in high density lipoprotein (HDL)-cholesterol, is well known. However, it remains uncertain whether phenobarbital increases plasma concentrations of cholesterol and triglyceride, and which subfractions of the high density lipoprotein fractions phenobarbital increases. The present study was therefore, conducted to investigate these points. Long-term treatment of phenobarbital increased the plasma concentrations of HDL2-cholesterol, HDL3-cholesterol and HDL3-phospholipid, and decreased the plasma concentrations of low density lipoprotein + very low density lipoprotein-cholesterol. On the other hand, its long-term treatment did not affect the plasma concentrations of cholesterol, triglyceride and phospholipid. In addition, phenobarbital increased the plasma concentration of apolipoprotein A-I, decreased the plasma concentration of apolipoprotein B, but did not affect the plasma concentration of apolipoprotein A-II. These results indicate the possibility that phenobarbital has an antiatherogenic effect.

Adult↗
PubMed1987

Malignant histiocytosis in infants: surface marker analysis of malignant cells in two cases.

Two infants with malignant histiocytosis, as diagnosed by morphological and cytochemical examinations of malignant cells infiltrating pleural effusion, skin, and bone marrow, are described. Surface marker analysis of the malignant cells with monoclonal antibodies showed these cells to express markers of T-lymphocytes and HLA-DR antigen, but not those of B-lymphocytes, granulocytes, or monocytes. These immunological findings demonstrated that the neoplastic cells in malignant histiocytosis of infancy may be of T-cell origin, rather than histiocytic or monocytic origin.

Antibodies, Monoclonal↗
PubMed1987

Structure and expression of cDNA for an inhibitor of blood coagulation isolated from human placenta: a new lipocortin-like protein.

An inhibitor of blood coagulation, a new protein with an apparent molecular weight of 34,000 and an isoelectric point of 4.9, was purified from human placental tissue by EDTA extraction. Five cDNA clones were isolated from the human placental lambda gt11 cDNA library using the mouse monoclonal antibody raised against the coagulation inhibitor as the probe. The longest insert consists of 1,566 nucleotides, and contains 960 nucleotides entirely encoding the 320 amino acids of the inhibitor, and a poly A tail. The deduced amino acid sequence was corroborated by chemical analyses of the protein. The entire amino acid sequence shows homology to those of lipocortin I, lipocortin II, and endonexin-related proteins. The cDNA for the inhibitor was expressed in Escherichia coli under the regulation of the trc promotor of the plasmid pKK233-2. The resulting recombinant protein manifested inhibitory activities against both blood coagulation and phospholipase A2 activity, as did the coagulation inhibitor isolated from human placenta.

Amino Acid Sequence↗
PubMed1987

Familial erythrophagocytic lymphohistiocytosis: surface marker analysis using monoclonal antibodies.

Surface marker analysis of atypical cells, present at elevated levels in the peripheral blood of a 2-yr-old patient with familial erythrophagocytic lymphohistiocytosis (FEL), was performed with monoclonal antibodies. The atypical cells expressed T11, T8, Ia and LeuM2 antigens, markers of suppressor/cytotoxic T lymphocyte and monocyte. The monoclonal antibody-defined surface markers were thus distinguishable from those of other lymphoproliferative disorders clinically resembling FEL.

Antibodies, Monoclonal↗
PubMed1987

Two types of phosphorylase from etiolated soybean cotyledons.

Two types of phosphorylase [EC 2.4.1.1] from the etiolated soybean (Glycine max) cotyledons were separated by column chromatography on DEAE-Sephacel and further purified to apparent homogeneities. Molecular weights of the subunits were 100,000 and 113,000 for phosphorylases I and II, respectively. The native enzymes I and II were a dimer (200,000) and tetramer (450,000), respectively. Electrophoretic analysis by the Hedrick and Smith method indicated that the two phosphorylases were distinct proteins with no correlation. The apparent Km values for glucose 1-phosphate, glycogen, and maltoheptaose of enzyme I were 4.00 mM, 0.18 mg/ml, and 10.3 mM, respectively, while those values of enzyme II were 5.43 mM, 23.8 mg/ml, and 0.30 mM, respectively. The relative activity of enzyme I increased with increasing chain length of the substrate glucans, and waxy maize amylopectin was the best substrate among the 11 saccharides examined. For enzyme II, maltooligosaccharides with degree of polymerization (DP) 5-7 were the better substrates than amylose (DP 38) and glycogen. These results indicated that the soybean phosphorylases I and II have similar properties to a cytoplasmic and chloroplastic type of plant leaf enzyme, respectively.

Chromatography, DEAE-Cellulose↗
PubMed1987

Eosinophilia in children. Cytochemical and immunological analysis of increased eosinophils.

Cytochemical and immunological studies of increased eosinophils were performed in three children with marked eosinophilia. These children were diagnosed as having non-Hodgkin's lymphoma, transient eosinophilia, and hyper-eosinophilic syndrome. All showed marked eosinophilia, with eosinophil counts of more than 5,000/mm3 in the peripheral blood and with eosinophils in all maturational stages in the bone marrow. In contrast to normal eosinophils, the increased eosinophils in three patients on cytochemical analysis showed moderately to strongly positive reactions for staining with alpha-naphthyl-butyrate esterase. Also, the mature eosinophils in the patient with lymphoma showed a strongly positive reaction for periodic acid-Schiff staining. The positive reaction for alpha-naphthyl-butyrate esterase and periodic acid-Schiff stain indicates the presence of eosinophilic proliferative disorders in these cases. Surface marker analysis of peripheral eosinophils demonstrated that an increased proportion of eosinophils expressed My7, a marker of granulocytes, in two patients with transient eosinophilia or hyper-eosinophilic syndrome, but not in a patient with lymphoma. In addition, 30-40% of eosinophils expressed Ia antigen, a differentiation marker of eosinophils, in two patients with lymphoma or hypereosinophilic syndrome. Thus, eosinophils in transient eosinophilia showed mature features, and those in hypereosinophilic syndrome showed immature features immunologically. Whereas, eosinophils in lymphoma may have a dysmaturity on the expression of Ia and My7. Cytochemical and immunological analyses of increased eosinophils may play an important role in clarifying the etiology of eosinophilia, especially its association with malignancy or other proliferative disorders.

Antigens, Surface↗
PubMed1986

Changes in the immunoreactivities of an opioid peptide leumorphin in the hypothalamus and anterior pituitary during the estrous cycle of the rat and their relation to sexual behavior.

Leumorphin, an opioid peptide whose functions are unknown, is found in mammalian brain and pituitary and stimulates lordosis behavior in estrogen-treated female rats. To elucidate the role of leumorphin in the physiological control of female sexual behavior, the levels of immunoreactive (ir) leumorphin as well as ir dynorphin (dynorphin A) were measured in the rat brain and pituitary during the estrous cycle. There was a clear variation of ir leumorphin in the hypothalamus and anterior pituitary during the estrous cycle. The levels of ir leumorphin in the hypothalamus and anterior pituitary on the afternoon of proestrus were significantly higher (P less than 0.01) than those on the afternoons of estrus and metestrus. The rise in the hypothalamic levels of ir leumorphin on the afternoon of proestrus was correlated with the receptivity of lordosis during the estrous cycle. Furthermore, there was a close correlation with ir dynorphin levels. These findings are in agreement with studies demonstrating a common precursor for leumorphin and dynorphin. Ir leumorphin in the hippocampus and neurointermediate pituitary did not change significantly during the estrous cycle. Because the leumorphin antiserum used recognizes rimorphin (dynorphin B) 1.78 times more than porcine leumorphin on a molar basis, high performance-gel permeation chromatography was done on pooled extracts of hypothalamus taken at proestrus and estrus. The peak in the leumorphin-like substance in the activation of sexual behavior is discussed.

Animals↗
PubMed1986

Immunofluorescent localization of structural collagen types in endochondral fracture repair.

A nonimmobilized rat tibial fracture model of endochondral osseous repair was examined for the unique localizations of specific collagen genetic types. At various stages of the healing process, the demineralized callus was reacted with immunofluorescent antibodies directed against the type specific forms of matrix collagen. Type III collagen rapidly appeared (day 8-10) and remained in the primitive mesenchymal callus until remodeled. It was particularly prominent in the highly vasoformative regions and the pericallus encapsulation but not present in preexisting cortical and neoformed lamellar bone. The type II collagen, a marker of cartilage, was uniquely located only in areas of chondroid differentiation and calcification. Type II collagen was absent from all bone and was not identified beneath the repairing intact periosteum. The differentiating chondrocytes synthesized type II collagen on an underlayer of type III collagen already within the mesenchymal matrix. From these studies of genetically unique collagen markers, it appears that only in areas of motion or anoxia does an intermediate of chondroid tissue appear. The utilization of specific type II and type III collagen immunofluorescent antibodies has facilitated the understanding of the fracture repair process and has acted as an indicator for unique matrix components.

Animals↗
PubMed1986

[Effect of isepamicin (HAPA-B) on reproduction. IV. Peri- and post-natal study in rats (intramuscular administration)].

Peri- and post-natal effect of HAPA-B, a new aminoglycoside antibiotic, was studied in Jcl: Wistar rats. The antibiotic was given intramuscularly to dams at doses of 25, 100 and 200 mg/kg from day 17 of gestation to 21 days after delivery and throughout lactation. Influences of the drug on dams and their offspring were studied. A decrease in food intake and an increase in water intake were observed in the 200 mg/kg treated group. At autopsy of dams after weaning, pale discoloration and hypertrophy of the kidney were observed in 100 and 200 mg/kg groups. All other observations including delivery and nursing performance of dams, postnatal development of offspring, behavior and reproduction performance of the offspring were normal. The no effect dose level of HAPA-B found in this study was 25 mg/kg on rat dams and 100 mg/kg on the offspring.

Animals↗