We report the phototoxicity of meso-tetrakis(4-sulphonatophenyl)porphine (TPPS4) and zinc metallocomplex (ZnTPPS4) sensitizers in the presence or absence of 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) on G361human melanoma cells. Morphological changes in cell cultures have been evaluated using inversion fluorescent microscope and image analysis. Viability of cells was determined by means of molecular probes for fluorescence microscopy (LIVE/DEAD kit- double staining with Calcein AM and Ethidium Homodimer). The quantitative changes of cell viability in relation to sensitizers concentrations and irradiation doses were proved by fluorometric measurement with fluoroscan Ascent. We found that the most effective sensitizer is ZnTPPS4 bound to HP-beta-CD, since the IC50 value was 12.5 g/ml at the dose of light radiation of 10 J/cm2.

Cell Line, Tumor↗

PubMedDate not supplied

Variability of haptoglobin and alpha 2-macroglobulin in Albino Oxford and Dark Augustin inbred strains of rats before and after trauma.

Increased synthesis of acute-phase proteins (APP) in the liver represents the most prominent aspect of acute phase reaction (APR). Haptoglobin (Hp) and alpha 2-macroglobulin (alpha 2-M) are among the most important APP in rats. In this study AO (Albino Oxford) and DA (Dark Augustin) inbred strains of rats, with different resistance to trauma, were used to compare concentrations of Hp, alpha 2-M, IL-1, IL-6 and TNF, before and after tourniquet trauma. Cytokines IL-1, IL-6 and TNF have an important role in APP gene expression. Our results showed that before trauma, more resistant AO rats had higher concentrations of Hp and alpha 2-M compared to DA rats. The influence of genetic elements of these differences is significant. Tourniquet trauma caused an early increase of IL-1, IL-6 and TNF activities in the serum which preceded the increase in the Hp and alpha 2-M concentrations but differences between strains were not found.

Animals↗

PubMedDate not supplied

Opinion of patients on the quality of hospital care.

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Health Services Research↗

PubMedDate not supplied

[Follow-up of congenital malformations: routine health statistics, a new registry or something else?].

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Congenital Abnormalities↗

PubMedDate not supplied

[Analysis of hospital mortality in the Croatian Republic using selected indicators].

Mortality trends and distribution in a system of 32 general and teaching hospitals in the Republic of Croatia for the years 1966 through 1989, separately for 1986/1987, were evaluated as a possible informational support to the national policies regarding developing of health care system. By means of 13 elaborated and/or selected indicators, using hospital health statistics as a data base, we measured the changes in the dynamics, structure and level of death at institutions, individual medical departments and among patients. Hospitals mortality statistics was found to offer a good informational orientation for monitoring realization of the hospital's restructuring activities envisaged under the Aim 29 of the Croatian strategy Health for All by the Year 2000 as well as for planning technical surveillance and streamlining the special statistical studies on inpatient health care. It can also provide a solid base for the evaluation of the quality of inpatient medical care, if the diagnosis of causes of death is improved, methodology for sentinel-health-events adopted, commissions for the evaluation of inpatient health care organized, medical registration units established and the National Register of Autopsy begun.

Adult↗