Examination of sectioned, embedded material showed that almost all intermediate filaments normally present in cultured chick embryo fibroblasts, IMR-33 cells, and BHK-21 cells were eliminated by trypsinization. At the same time atypical filaments of intermediate size were formed by close apposition of thin filaments. Previous observations (Buckley et al., 1978, J. Cell Biol. 78:644-652) on negatively stained and on critical-point-dried whole-cell mounts confused these different filament types and, on the basis of heavy meromyosin binding to the atypical intermediate-sized filaments, we suggested that many normal intermediate filaments contain f-actin. The present results show that this suggestion was unwarranted and we therefore retract it.

Actins↗

PubMed1981

Amelanotic lentigo maligna.

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Adult↗

PubMed1981

The role of N-nitrosamine in carcinogenesis at the ureterocolic anastomosis.

Seven cases of colonic tumours occurring at the ureterocolic anastomosis (UCA) in patients diverted for benign disease are presented and comparisons made with existing literature. There appears to be a definite increase in the incidence of colonic malignancy in patients with this diversion. A theory of carcinogenesis involving bacterial activation of endogenously formed N-nitrosamine is suggested. Preliminary results of rectal urine analysis support this theory in that high concentrations of N-nitrosamine have been found mutagens demonstrated.

Adenocarcinoma↗

PubMed1981

Urethral diverticula in the adult female.

Forty female patients with persisting urinary symptoms were investigated using positive pressure urethrography. Sixteen patients were found to have urethral diverticula; 14 of these underwent diverticulectomy. In addition, 17 patients were found to have a markedly distensible urethra on X-ray and only the remaining 7 were deemed normal. The clinical and radiological features and surgical management of these patients are presented.

Adult↗

PubMed1981

Scanning electron probe x-ray microanalysis of elemental distributions in freeze-dried cryosections of Bacillus coagulans spores.

High-resolution scanning electron probe X-ray microanalysis had been employed to examine elemental distributions in freeze-dried cryosections of Bacillus coagulans spores. Calcium, manganese, and phosphorus were concentrated in the protoplast and the coat. Iron was found in the coat but not in the protoplast, whereas the silicon seen on the coat of other spore species was absent. Sulfur was present in the coat, but was distributed over a broader area than the other elements, which suggested that phosphorus and the metal ions were located in the outer coat layer.

Bacillus↗

PubMed1981

Urinary beta 2 microglobulin in the biological monitoring of cadmium workers.

Urinary beta 2 microglobulin (beta 2m) estimation has been added to an existing pre-employment and periodic medical surveillance programme for cadmium workers. Pre-employment values were measured in 203 men not occupationally exposed to cadmium. The overall geometric mean was 76 microgram/l (adjusted to specific gravity 1016): a significantly higher level of 96 microgram/l was found in the specimens stored continually at -20 degrees C after voiding, compared with 73 microgram/l in specimens that thawed during transport. Sodium azide had been added to all specimen bottles. Employees exposed to cadmium pigments at various stages of their manufacture had no evidence of raised urinary beta 2m despite exposures above 50 microgram/m3 for up to 11 years. This is believed to be due to the insolubility of the compounds. Five known cases of cadmium induced proteinuria whose exposure ceased up to 15 years ago had raised urinary beta 2m concentrations. Moderately raised concentrations were found in seven others with a history of cadmium oxide exposure and in whom proteinuria has never been detected. The place of urinary beta 2m in the health care of cadmium workers is discussed and the question of correct management of a cadmium worker with a high beta 2m is raised.

Adolescent↗

PubMed1981

Nursing the community.

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Aged↗

PubMed1980

Prostacyclin, indomethacin and the cerebral circulation.

The effect of intracarotid prostacyclin (PGI2) on cerebral blood flow (CBF) was measured by the 133xenon intracarotid injection technique in 8 baboons. Intracarotid prostacyclin increased CBF by 22% at 10(-7) g/kg/min and by 71% at 5 x 10(-6) g/kg/min, accompanied by systemic hypotension and tachycardia. The effects of PGI2 (10(-7) g/kg/min) were not potentiated by transient opening of the blood-brain barrier with the intracarotid hypertonic urea technique. At hypercapnia, the vasoconstrictor effect of indomethacin on the cerebral circulation was reversed by PGI2. These results support our suggestion that a prostaglandin, in particular PGI2, is required for hypercapnia to produce full cerebral vasodilatation. In separate experiments, following craniectomy in 5 cats, PGI2, but not its stable metabolite 6-keto-PGF1 alpha, dilated pial arterioles when locally injected into the mock CSF overlying the arteriole.

Animals↗