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Biomedical subjects

M Stern

Publications and source records attributed to M Stern.

At least 289 records · Page 16Linked to original sources
PubMed1982

[Cow's milk protein antibodies determined by immunofluorescence (RIFT) in cow's milk protein intolerance and in controls (author's transl)].

An immunofluorescent method using antigencoupled red cells was used to determine antibody titres against five main cow's milk proteins in 20 children with CMPI, in 130 normal controls, and in 70 children with various gastrointestinal disorders. Antibody findings were related to age and nutritional conditions. For no antibody group, a pathogenetic significance was found with respect to antigen or immunoglobulin class. Formation of serum cow's milk antibodies was far from indicating a pathological process. Nevertheless, determination of cow's milk antibody titres by RIFT had some use in the differential diagnosis of CMPI, although in comparison it has not the specific of gliadin antibodies in coeliac disease. Combined clinical and morphological criteria for the diagnosis of CMPI can not be replaced by immunofluorescent cow's milk antibody testing.

Adolescent↗
PubMed1981

Polymeric 2-mercaptopyridine and 2-mercapto-nitrobenzene derivatives. New reagents for peptide synthesis.

Insoluble 2-mercaptopyridine and 2-mercapto-nitrobenzene derivatives were prepared by modification of commercially available polystyrene. Applicability of these polymers as reagents for the thiolytic removal of the 2-nitrophenylsulphenyl amino-protecting group and as supports for preparation of polymeric active esters was evaluated. Polymeric 2-mercaptopyridine (PMP) was found efficient for both purposes. It was used in the stepwise synthesis of Leu-enkephalin via the polymeric reagent approach, serving as an Nps-cleaver. Polymeric esters derived from PMP and Boc-amino acids proved to be excellent acylators. Their usefulness is exemplified in the preparation of two dipeptides, which were produced rapidly and in high yields and purity.

Endorphins↗
PubMed1981

Total aganglionosis of the colon (Hirschsprung's disease) and congenital failure of automatic control of ventilation (Ondine's curse).

Total aganglionosis of the colon presenting with small intestinal obstruction in the neonatal period was observed in combination with congenital alveolar hypoventilation requiring continuous mechanical ventilation in a boy. The patient died aged 15 months from acute dehydration due to enteritis, long after total resection of the aganglionic bowel had been performed. Pulmonary hypertension was found in the newborn period. There was progressive right ventricular myocardial hypertrophy. This is the fourth case reported with a combination of defects involving nerve cell function of the brain stem and gastrointestinal tract.

Colon↗
PubMed1981

LHRH and analogues as potential therapy for benign prostatic hyperplasia and hormone-dependent cancers.

In adult male rats, daily s.c. injections of 0.008-125 microgram luteinizing hormone releasing hormone (LHRH) or its analogue (D-Ala, des-Gly-NH2)LHRH ethylamide, led to significant differences in inhibitory effects on testicular function and accessory sex organ weights. The analogue was at least 35 times more potent than LHRH in reducing testicular LH/hCG receptors and 350 times more effective in decreasing plasma testosterone concentrations. In a second study, adult male rats treated with 25-2500 microgram LHRH daily for a 3-week period showed 85%-90% decrease in plasma testosterone concentrations. Treatment with 25 or 250 microgram LHRH effected a maximal 25% and 40% decrease of the ventral prostate and seminal vesicles, respectively, without affecting the testicular weight, the latter being reduced by 15% with the higher dose of 2500 microgram. The LHRH analogue is proposed for the treatment of prostatic and breast cancers while native LHRH could be an effective therapy for benign prostatic hyperplasia.

Animals↗
PubMed1981

[Cow's milk protein intolerance: clinical and pathogenic aspects (author's transl)].

Cow's milk protein intolerance is a transient food intolerance of early infancy. Ingestion of cow's milk protein causes an enteropathy of variable degree. Clinical manifestations are primarily gastrointestinal, although dermal and respiratory symptoms add to the clinical syndrome. Three types are found: an acute anaphylactic reaction, a chronic mild form, and a chronic severe form which is of utmost practical importance in severe protracted diarrhoea of infancy. A graduated diagnostic procedure is proposed, taking into account clinical and morphological reactions to cow's milk proteins. Pathogenesis is immunologically mediated. A concept of transient food protein intolerance is developed which has to be separated from the permanent intolerance of gluten in coeliac disease. Prognosis of cow's milk protein intolerance is excellent after elimination of the offending agent. Breast feeding seems to be effective in prevention of the disease.

Anaphylaxis↗
PubMed1980

Protracted diarrhoea: secondary monosaccharide malabsorption and zinc deficiency with cutaneous manifestations during total parenteral nutrition.

Protracted diarrhoea in early infancy gives rise to many diagnostic and therapeutic problems. Jejunal biopsy often reveals villous atrophy of varying degrees. Severe reduction of small intestinal absorptive area causes secondary monosaccharide malabsorption, as well as secondary disaccharide deficiency, consequences which are relevant in any attempts at oral feeding. Morphologic, metabolic, endocrinological and microbiological studies have to be undertaken in order to establish a definitive diagnosis in protracted diarrhoea, but these studies often fail to reveal the aetiology of the disease. Immunologic abnormalities like phagocyte dysfunction, thymic atrophy and hypoplasia of B-cell regions in lymph nodes might be secondary events, but some types of immunodeficiency are of primary importance in the development of protracted diarrhoea. Total parenteral nutrition in many cases has to be instituted, with all its implications and hazards: septicaemia is the most dangerous of these. Zinc deficiency and acrodermatitis enteropathica may occur during total parenteral nutrition, and zinc deficiency secondarily contributes to the symptoms of diarrhoea. Parenteral administration of zinc is able to overcome these effects.

Acrodermatitis↗
PubMed1980

[Coeliac disease: clinical and pathogenic aspects (author's transl)].

Coeliac disease is a permanent food intolerance with a genetic basis which persists throughout the whole life. Ingestion of gluten proteins (wheat, rye, barley, oats) causes atrophy of the jejunal villi and, as a consequence, malabsorption. Diagnosis can only be proved by three consecutive intestinal biopsies; initially on normal diet, after 12--18 months of gluten-free diet, and after a final challenge with gluten-containing food. Biochemical changes have been discussed for a long time to be of primary importance in the pathogenesis of coeliac disease. Recently, however, evidence is increasing that immunological mechanisms are primary factors in the development of the disease. A synopsis of biochemical and immunological phenomena and of membrane receptor alterations of enterocytes and immunocytes which are genetically based is more likely to answer the question of pathogenesis than any single theory. Therapeutically, life-long gluten-free diet is necessary. In some cases, after a long course the prognosis is limited by the increased incidence of malignancy.

Celiac Disease↗
PubMed1980

Pelvic kidney masquerading as an adnexal mass.

A case of a pelvic kidney diagnosed as an adnexal mass, resulting in surgery, is reported. The employment of ultrasonography in the evaluation of suspected pelvic masses is well established. A number of pathologic conditions can be responsible for a pelvic mass in female patients and consideration of the differential diagnosis of such masses has been well documented. The discrimination of a pathologic pelvic mass from an anatomic variation or unusual location of a normal structure can be, however, a potential source of confusion and erroneous interpretation. This report concerns the unfortunate result of not considering a renal anomaly, i.e. ectopic pelvic kidney, in the differential consideration of a "pelvic mass".

Adnexal Diseases↗
PubMed1979

Immunofluorescent serum gliadin antibodies in children with coeliac disease and various malabsorptive disorders. I. Technique, clinical evaluation and diagnostic use of a gliadin antibody assay using pyruvic aldehyde-treated human red cells.

An immunofluorescent gliadin antibody assay is described using pyruvic aldehyde-stabilized human erythrocytes coated with gliadin. Fifty coeliac children all had high serum IgG-antigliadin titres during a normal diet or a challenge with gluten. On a gluten-free diet (30 children), titres were much lower. In patients followed-up for one year on a gluten-free diet, an initial rise in titres was followed by a slow decline. On challenge, IgG-antigliadin titres showed a slow rise or persistence at the same level in most patients. Fifty-two percent of control children with malabosrptive disorders, but without the typical "flat" mucosal lesion on jejunal biopsy, were shown to have positive titres in their sera, as were 6% of normal children and 4% of adult blood donors. The fluorescent antibody technique was compared with methods commonly used to detect wheat-protein antibodies, and was found to be superior to all of them. The immunofluorescent gliadin antibody assay appears to be useful in following-up children with coeliac disease, and in selecting patients for jejunal biopsy, although it does not replace biopsy.

Antibodies↗
PubMed1979

Shwachman's syndrome and leukaemia.

The clinical and morphological characteristics of Shwachman's syndrome (exocrine pancreatic insufficiency, pancytopenia, skeletal changes) were observed in a boy who, at the age of 8 years, developed a juvenile form of chronic myeloic leukemia which did not respond to cytostatic treatment. Autopsy revealed a striking lipomatous atrophy of the pancreas, defects in the ossification zones of the bones and marked dwarfism. In addition there was leukaemic infiltration of the pancreas, the spleen, the liver and the lymph nodes. The association of Shwachman's syndrome with leukaemia is a rare, but remarkable complication of this entity because of its relationship to the preceeding pancytopenia. Thorough follow-up of the haematological status of patients with Shwachman's syndrome is recommended.

Autopsy↗