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Biomedical subjects

M St Pierre

Publications and source records attributed to M St Pierre.

31 records · Page 2Linked to original sources
PubMed1996

Cellular response to a multiple-metal stress in Pseudomonas fluorescens.

Pseudomonas fluorescens multiplied in a minimal mineral medium supplemented with millimolar amounts of aluminum (5 mM), iron (5 mM), zinc (3 mM), calcium (2 mM) and gallium (1 mM). A slight decrease in growth rate and a 22% diminution in cellular yield were observed as compared to the control medium. Citrate, the sole source of carbon to which the test metals were complexed, was completely utilized. Although at stationary phase of growth most of the metals were immobilized in an exocellular lipid-rich residue, ultracentrifugation and dialysis studies revealed that metals were associated with phosphatidylethanolamine (PE) from early stages of growth. As growth progressed the metal content of the soluble cellular extract increased reaching an optimum at 35 h of incubation. However, no detectable amounts of metals in this cellular component were discerned at stationary phase of growth. There appeared to be no marked variation in exocellular protein and carbohydrate production in control and metal-stressed cultures. Transmission electron microscopic studies revealed metal rich bodies associated with the cytoplasm. Scanning electron microscopic analyses of the dialyzate aided in the identification of the metal-rich bodies associated with elongated structures comprised of carbon, oxygen and phosphorus. PE appeared to be an important organic constituent of the gelatinous residue.

Biotechnology↗
PubMed1996

Diversity in delivery: the Medicare home health benefit.

Most home care providers know that Medicare covers home care nursing; home care aide and medical social services; physical, speech, and occupational therapy; as well as medical supplies and durable medical equipment. However, few agencies realize that they can also use dietitians and respiratory therapists to help meet their patients' needs. Also, few agencies use available resources or establish programs to deliver care to special-needs populations. Yet all of these home care services are reimbursable under the Medicare home health benefit.

Aged↗
PubMed1995

HCFA's home health initiative: the first comprehensive reassessment of the Medicare home health benefit.

Last year the Health Care Financing Administration announced an exciting program: the HCFA Home Health Initiative, which provided the home care industry an opportunity to contribute to changes in the way Medicare operates. For a year the Initiative pushed forward with goals and meetings. The newly elected members of Congress, however, have revealed many plans for cutting back on spending as well as government regulation. What does that mean for the Initiative and the Medicare home health benefit?

Centers for Medicare and Medicaid Services, U.S.↗
PubMed1993

Dubin-Johnson-like syndrome in golden lion tamarins (Leontopithecus rosalia rosalia).

On routine blood screens, persistent conjugated hyperbilirubinemia was discovered in two groups of closely related adult female golden lion tamarins (Leontopithecus rosalia rosalia, n = 8). Bromosulfophthalein (BSP) retention tests were performed on four hyperbilirubinemic and three control tamarins. BSP excretion was delayed in hyperbilirubinemic tamarins as compared with controls. Grossly, liver of affected tamarins was dark brown to black, with a prominent reticulated pattern. Histologic examination revealed abundant intrahepatic pigment, primarily in a centrilobular and midzonal distribution. Most of the pigment did not react with Perls' Prussian blue method for iron, Hall's method for bilirubin, or the Armed Forces Institute of Pathology acid-fast method for lipofuscin but was positive with Fontana and lipofuscin-ferric ferricyanide reduction techniques. Liver from control golden lion tamarins had intrahepatocellular Perls' iron-positive pigment diffusely throughout the lobule with a small amount of Fontana method-positive pigment. Ultrastructurally, hepatocytes from a hyperbilirubinemic tamarin contained pleomorphic electron-dense structures within lysosomes. Transport studies demonstrated secretion of fluorescein isothiocyanate-labeled glycocholic acid, a fluorescent bile acid analog, into bile canaliculi and no secretion of carboxydichlorofluorescein diacetate, a non-bile acid organic anion, by liver from a hyperbilirubinemic tamarin. In contrast, control liver secreted carboxydichlorofluorescein diacetate readily into bile canaliculi. The clinicopathologic presentation of this syndrome in golden lion tamarins is similar to that described for Dubin-Johnson syndrome of human beings.

Animals↗
PubMed1981

Tumor and placental histaminase, I. affinity chromatography purification and characterization of the placental enzyme.

Histaminase (diamine oxidase) is an enzyme produced at very high levels by the decidua of the placenta and is found to be associated with a number of human cancers. A procedure for the affinity chromatography purification of this enzyme is described. In this procedure, cadaverine-AH-sepharose was used to bind the enzyme in the placental extract. After extensive washing of the column with 2.5% Triton X-100 in 1 M NaCl, the enzyme was released from the column by 0.1 N chromotropic acid. This purification, essentially a one step procedure, provided 1800-fold purification, and yielded mg quantities of histaminase, homogeneous by SDS-gel electrophoresis and immunodiffusion tests. The procedure usually recovered more than 40% of the enzyme applied and the specific activity of the final enzyme preparation was around 5000 units/mg protein. SDS-gel electrophoresis of the enzyme in different concentrations of acrylamide indicated that the subunit molecular weight of histaminase was about 90,000. Isoelectric focusing of the enzyme in polyacrylamide gel revealed 5 major enzyme components. Results of amino acid analyses indicated that the enzyme had a low content of sulfur-containing amino acids and a relatively high content of dicarboxylic amino acids. The availability of this purification will be useful for the development of immunological methods for detections and quantitation of this enzyme in specimens from cancer patients.

Amine Oxidase (Copper-Containing)↗