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Biomedical subjects

M Smith

Publications and source records attributed to M Smith.

At least 1,459 records · Page 81Linked to original sources

Oligodeoxyribonucleotides: chemical synthesis in anhydrous base.

The sugar hydroxyl of a methoxytrityldeoxyribonucleoside reacts with a methoxytrityldeoxyribonucleoside phosphorofluoridate in dimethylformamide and potassium tertiary butoxide to yield the protected dinucleoside monophosphate. The reaction is fast and specific, and is used in a stepwise synthesis to prepare trinucleoside diphosphate and tetranucleoside triphosphate.

Chromatography, Ion Exchange↗