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Biomedical subjects

M Smith

Publications and source records attributed to M Smith.

At least 1,405 records · Page 78Linked to original sources
PubMed1974

Amino acid content of rabbit acrosomal proteinase and its similarity to human trypsin.

Rabbit acrosomal proteinase from epididymal spermatozoa of 44 male rabbits was purified by subcellular fractionation, sucrose density gradient centrifugation, and electrofocusing; the specific activity of the purified product was 20,047 units per milligram, a value similar to that observed for pancreatic trypsins from various sources. The molecular weight determined from the amino acid analyses and ultracentrifugation was about 22,000. This rabbit acrosomal proteinase showed great similarity to pancreatic trypsin, especially to human pancreatic trypsin, both in the number of individual amino acids and in the total number of residues. This similarity was confirmed by an antigenic cross reaction between rabbit antiserum to bovine pancreatic trypsin with human, rabbit, rhesus monkey, and bull acrosotnal proteinase.

Amino Acids↗
PubMed1974

Mesentericocaval "jump" graft in management of portal hypertension: experience with 24 cases.

A series of 24 patients with cirrhosis have undergone mesentericocaval shunt operations for the relief of portal hypertension. Overall the results have been satisfactory. Four of the five patients treated as an emergency and 17 of the 19 who had the operation two to six weeks after haemorrhage had been controlled left hospital alive and well. Separation of the patients into three categories according to the findings of clinical and biochemical tests, however, showed that subsequent survival was satisfactory for patients in categories A and B but that all four patients in category C had died within one year after surgery. Assessment at three months showed that in three patients moderate hepatic encephalopathy had developed.Evidence that the shunt remains patent was shown by a low incidence of repeated gastrointestinal haemorrhage and a marked diminution in variceal size in 18 of the 19 cases examined serially. Radiographic techniques for confirming shunt patency were compared and cannulation of the graft via the femoral vein was found to provide a reliable and rapid means of assessment.

Adult↗
PubMed1974

Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme.

A modified purification is described for an enzyme, from Escherichiacoli B, which polymerizes deoxyribonucleoside-5' diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligonucleotide primer. At all stages, the enzyme activity copurified with the activity which will polymerize adenosine-5' diphosphate (polynucleotide phosphorylase). Studies of heat stability, the effect of various temperatures of reaction and of disc gel electrophoresis failed to provide evidence that the two activities are separable.

Base Sequence↗
PubMed1974

Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Deoxyribo-oligonucleotide synthesis.

An enzyme, which is probably identical with polynucleotide phosphorylase, was prepared from Escherichiacoli B. In the presence of Mn(2+) it catalyzes the addition of one (and to a slight extent more) residue of deoxyribonucleotide residue from the diphosphate to an oligodeoxyribonucleotide primer. The shortest effective primers contained three phosphate residues. Ribodinucleotides were effective as primers and accepted two or three deoxyribonucleotide residues under these conditions. The application of the procedures to the convenient synthesis of certain defined oligodeoxyribonucleotides up to nine residues long is discussed.

Base Sequence↗