A 40-year-old woman was found to have a well-circumscribed mass in the upper outer quadrant of the right breast at a routine physical examination. Mammographically, the tumor was a radiolucent mass, measuring 4.6 X 3.2 X 4 cm, surrounded by a capsule. The tumor consisted of fat and numerous strings with the appearance of ordinary glandular tissue. There was no area of microcalcification. Grossly, the tumor mass was encapsulated by fine connective tissue. On the cut surface, the tumor consisted of fatty tissue containing islands of grayish-white breast tissue. Histologically, the fatty tissue presented (a feature) of mature lipoma. Embedded in the fat were areas of breast tissue simulating fibrocystic disease. In limited areas, the fat cells had direct contact with the ducts without intervening fibrous tissue.

Adult↗

PubMed1984

Plasmid vectors designed for high-efficiency expression controlled by the portable recA promoter-operator of Escherichia coli.

A novel expression vector using the 236-bp promoter-operator fragment of the recA gene (recApo) of Escherichia coli has been constructed. This DNA fragment contains complete signals for the initiation of RNA synthesis, as well as for regulation by the lexA product, but lacks the coding sequence for the RecA protein. The strength of the recA promoter was examined by assaying beta-galactosidase activity expressed from a cro-lacZ fused gene placed downstream of the promoter. Under noninducing conditions, the promoter was regulated by the LexA protein, and the fused gene was expressed only weakly. Upon induction by nalidixic acid in a recA+ strain, high expression was observed for an extended period. After 5 h under inducing conditions, as much as 11% of the total cellular protein was cro-lacZ product. The expression level was higher than that from promoters of lac, trp, and lambda early genes.

Bacterial Proteins↗

PubMed1984

Experimental studies on the effects of Mt. Sakurajima volcanic ashes on the respiratory organs.

Since 1978, the authors have collected ashes from Sakurajima volcanic eruption in Kagoshima City, and the ashes were administered to rats and rabbits through different routes and forms injecting into the trachea in order to see the effect of ashes on the respiratory organs. The authors experimentally and histopathologically demonstrated bronchitis, pulmonary emphysema, atelectasis lung, degeneration of blood vessel, dust nodes and induction of pneumoconiosis due to dust fibrosis in the study of the effect of volcanic ashes on the respiratory organs.

Animals↗

PubMed1984