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Biomedical subjects

M Shibasaki

Publications and source records attributed to M Shibasaki.

188 records · Page 11Linked to original sources
PubMed1978

Vasodilation produced by prostacyclin (PGI2) and 9(O)-thiaprostacyclin (PGI2-S) in the canine femoral circulation.

The action of 9(0)-thiaprostacyclin (PGI2-S) was compared with that of prostacyclin (PGI2) and papaverine in the femoral circulation of dogs. PGI2-S, injected into the dog femoral artery in a dose of 0.1 microgram or higher, produced marked vasodilation in the femoral artery without any change in blood pressure. The potentcy of PGI2-S was one tenth that of PGI2, and was a hundred times that of papaverine. The stability of PGI2-S in neutral solution was forty times that of PGI2.

Animals↗
PubMed1978

Induction of lymphocyte colony formation in vitro by protein A.

Human lymphocyte colonies were grown in semi-solid culture in the presence of staphylococcal protein A. The best results were obtained when 1 X 10(6) lymphocytes were cultured for 5 to 7 days with 25 to 50 microgram of protein A per dish. A lineal relationship was observed between the number of cells seeded and the number of colonies developed. An increased number of colonies was seen in cord blood culture compared with that in child and adult peripheral blood. The addition of 5 X 10(-5) M of 2-ME to the culture approximately doubled the colony counts. The cells harvested from the colonies contained about 70% of E-RFC and 20% of EAC-RFC.

Adult↗
PubMed1976

A case of mosquito allergy. Immunological studies.

A girl aged 14 years, who showed local skin lesions and generalized reactions such as fever, hepatosplenomegaly, hypergammaglobulinaemia and pancytopenia following mosquito bites, has been evaluated with various immunological methods. The intracutaneous tests, P-K tests and RAST confirmed the presence of specific IgE antibody against mosquito antigens, but no precipitating antibody was detected by either the double immunodiffusion or immunofluorescent antibody techniques. As far as the type IV reaction is concerned, the skin test failed to show a positive delayed skin reaction, but the lymphocyte incorporation of 3H-TdR exhibited a maximal response with smaller doses than the controls.

Adolescent↗
PubMed1976

Discrepancy between skin reactivity and blastogenic activity of fractionated PHA-P.

Firstly, it was reported that phytohemagglutinin P (PHA-P) can induce the immediate skin reaction besides the delayed one. 70 out of 80 cases (87.5%) gave positive immediate reactions with 1 mug of PHA-P. There was a close correlation between the radioallergosorbent test (RAST) counts against PHA-P and the total IgE value or erythematous diameter of the immediate skin reactions. Absorption with anti-IgE or PHA-P reduced the RAST counts against PHA-P to 79 and 56% of their initial values, respectively, but did not effect the RAST counts against steptokinase-streptodornase (SK-SD). These results suggest that the immediate skin reaction might be induced by specific IgE antibody against PHA-P. Secondly, using three fractions of PHA-P obtained by Sephadex G-200 gel filtration, skin test and RAST in humans, ACA in rats, and 3H-thymidine incorporation into human leukocytes, were carried out and data obtained that showed some discrepancy between them. That is, on skin testing, it was shown that all of the three fractions (fr.) had the ability to elicit the immediate and delayed skin reaction in humans and rats with the order of fr. 1, 2 and 3, and it was confirmed by RAST. On the other hand, the leukocyte-stimulating activity resided in fr. 1 and 2, while fr. 3, which seemed to be a group of subunits with a molecular weight of around 35,000, had almost no activity. These findings have been discussed in view of the earlier studies on the structure of PHA-P

Absorption↗
PubMed1976

Inhibition of 3H-thymidine incorporation into human lymphocytes by dialyzable material of streptokinase- streptodornase.

Using the 3H-thymidine incorporation test, the lymphocyte-stimulating activity of streptokinase-streptodornase (SK-SD, Varidase) and its fractions were evaluated. The dose-response curves of all the preparations except the third fraction had two peaks with the maximum around 5 and 40 mug. The dialyzable fraction of Sk-SD (D-SK-SD) occupying approximately half of the original sample had almost no lymphocyte-stimulating activity; and conversely, it had a potent inhibitory effect on the 3H-thymidine incorporation into lymphocytes that normally occurs with non-dialyzable SK-SD (ND-SK-SD), PPD and PHA-P. Therefore, it will be desirable to remove the dialyzable fraction from the commercial SK-SD preparation when it is used for the determination of the capacity of cellular immunity against tsk-sd.

Dialysis↗
PubMed1976

Specificity of immediate skin reaction with streptokinase-streptodornase (SK-SD) in humans.

Streptokinase-streptodornase (SK-SD) induced immediate and delayed skin test reactions in a high proportion of patients with disorders in which beta-streptococcal infection may be involved. The P-K test and absorption experiments with antigen or anti-IgE suggested that the immediate reaction was mediated by specific IgE antibody against SK-SD. Furthermore, the ratio of specific IgE antibody against SK-SD to total IgE was roughly calculated to be 20 percent. Finally, the RAST technique was applied to detect specific IgE antibody against SK-SD which showed high radiocounts bound to SK-SD-coupled particles in the sera of nephrotic patients who had a strong immediate reaction against SK-SD and a severe disease state.

Adult↗