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Biomedical subjects

M Senba

Publications and source records attributed to M Senba.

At least 55 records · Page 3Linked to original sources
PubMed1988

Eosinophilic globules in pheochromocytoma of the adrenal medulla. A histochemical, immunohistochemical and ultrastructural study.

Histochemical, immunohistochemical, and ultrastructural characteristics of eosinophilic globules in pheochromocytoma of the adrenal medulla are described. The globules were observed in 7 (63.5%) out of 11 cases of pheochromocytoma of the adrenal medulla. These globules were eosinophilic, PAS-positive with and without diastase predigestion, phosphotungstic acid hematoxylin (PTAH) positive, and autofluorescent under ultraviolet illumination. Although almost all globules were not stained with neuron specific enolase (NSE) using the immunoperoxidase method, a few globules were stained positive. The lectins of Triticum vulgaris (WGA) and Ricinus communis (RCA-120) were weakly bound to most of the eosinophilic globules. Immunohistochemical reactions for alpha-fetoprotein (AFP), alpha-l-antichymotrypsin (ACT), and human chorionic gonadotropin (HCG) revealed negative reaction in these globules. An ultrastructural study revealed no relationship between these globules and chromaffin secretory granules. These findings suggested that the eosinophilic globules in pheochromocytoma of the adrenal medulla were not related to the chromaffin secretory granules and might be some kind of complex protein.

Adrenal Gland Neoplasms↗
PubMed1988

Eosinophilic globules in fibroadipose tissue adjacent to Kaposi's sarcoma.

Eosinophilic globules have been reported in the sarcomatous tissue of the cutaneous type of Kaposi's sarcoma. This report presents a case in which similar eosinophilic globules were found in uninvolved area of fibroadipose tissue adjacent to the sarcomatous tissue in a 30-year old African woman. The histochemical characteristics of these globules were identical to those found within the sarcomatous tissue. The origin of these globules is not known, but a possible explanation is offered.

Adipose Tissue↗
PubMed1988

Black thyroid associated with minocycline therapy: histochemical and ultrastructural studies on the brown pigment.

The brown pigment found in the black thyroid, associated with minocycline therapy, was studied histochemically and ultrastructurally. The brown pigment was Fontana-Masson silver-positive, and was negative for iron, for autofluorescence under uv illumination, for periodic acid Schiff (PAS) and for acid-fast iodine peroxidase (AFIP) lipofuscin, and was bleached with potassium permanganate. The light and electron microscopic studies suggested that the pigment resembled melanin. Although the mechanism of the pigment deposition is not clear, a possible explanation may be that the pigment is related to direct oxidative degradation products of minocycline.

Aged↗
PubMed1987

A staining method for easy detection of eosinophilic globules in Kaposi's sarcoma.

Eosinophilic globules provide an important clue for diagnosis of Kaposi's sarcoma (KS). These globules were stained with periodic acid Schiff (PAS), periodic acid Schiff reagent after diastase digestion, phosphotungstic acid hematoxylin (PTAH) and autofluorescence under ultraviolet illumination. By these methods, however, eosinophilic globules in KS could not be readily identified. Therefore, we developed a new procedure for rapid identification of eosinophilic globules in KS tissues.

Humans↗
PubMed1986

Rapid identification of Klebsiella pneumoniae in the lung tissue by fluorescence microscopy.

Klebsiella pneumoniae is stained weekly with hematoxylin and eosin, moderately with periodic acid Schiff (PAS), and strongly by the Warthin-Starry method. Staining by the Warthin-Starry method, however, takes more than one hour. Therefore, we developed a new procedure for rapid identification of K. pneumoniae in paraffin sections of the lung under a fluorescence microscope.

Animals↗
PubMed1986

A rapid silver staining method for identification of Mycobacterium leprae in histologic sections.

A few methods have been reported for the purpose of staining Mycobacterium leprae in paraffin sections, including Fite oil fuchsin method, auramine-rhodamine method, and Blanco-Fite silver method. Among these staining techniques, Fite oil fuchsin method and auramine-rhodamine method are popular. However, the Blanco-Fite silver method takes approximately 20 days. Therefore, we developed a new procedure for rapid identification of M. leprae in paraffin sections using another silver solution and found that the procedure gave stable and satisfactory results. This new method has proved superior to others in demonstrating a reliable staining for M. leprae.

Humans↗
PubMed1986

Eosinophilic globules in Kaposi's sarcoma. A histochemical, immunohistochemical, and ultrastructural study.

Of the 12 cases, 2 (17%) showed eosinophilic globules in the typical cutaneous type of Kaposi's sarcoma. The globules were stained with periodic acid-Schiff (PAS), periodic acid-Schiff reagent after diastase digestion, and phosphotungstic acid hematoxylin (PTAH), but were not stained with Mayer's mucicarmine, and alcian blue. As the results, these globules might be glycoprotein. The shape of the globules was very similar to glycoprotein globules of yolk sac tumor (endodermal sinus tumor) in the tissue of the ovary and testis. In the yolk sac tumor, similar globules are stained with alpha-fetoprotein, beta-subunit of human chorionic gonadotropin, and alpha-1-antitrypsin using immunohistochemical techniques. Immunoperoxidase investigations were done with antibodies to alpha-fetoprotein, beta-subunit of human chorionic gonadotropin, alpha-1-antitrypsin, and carcinoembryonic antigen in the eosinophilic globules of Kaposi's sarcoma, but these antigens were detected in the globules. The morphogenesis of the glycoprotein globules is not clear yet. A better understanding of the source of globules in Kaposi's sarcoma awaits further research.

Carcinoembryonic Antigen↗
PubMed1985

Statistical analysis of relationship between iron accumulation and hepatitis B surface antigen.

Statistical analysis was carried out to determine the relationships of iron deposition among hepatocytes, Kupffer cells, bile duct epithelial cells, portal tracts, and spleens using 68 autopsy cases obtained from Kenya, which included cases of Bantu siderosis. Of 68 cases, 33 (49%) showed siderosis. Cirrhosis was observed in 19 cases, of which 10 (53%) showed siderosis. However, there was no correlation between iron deposition and cirrhosis (Pearson chi 2 = 0.6734). The finding suggests that iron accumulation does not lead to cellular injury. On the other hand, there was a strong association between iron deposition in the livers and that in the spleens. There was also a strong correlation between the presence of hepatitis B surface antigen and iron deposition in the Kupffer cells and in the spleens. Therefore, it is suggested that the hepatitis B virus infection affects the iron metabolism of the reticuloendothelial system.

Female↗