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Biomedical subjects

M Semczuk

Publications and source records attributed to M Semczuk.

At least 37 records · Page 2Linked to original sources
PubMed1997

Frequency of HPV infection and GSH levels in plasma of women with cervical dysplasia.

Human papillomavirus (HPV) can be a cause of cervical intraepithelial neoplasia (CIN) and cervical cancer. The level of antioxidant compounds in plasma (retinol, alfa-tocoferol, vitamins C and E, glutathione) can modulate the progression of latent HPV infection to subclinical lesions and CIN. Our studies show that in the cases of HPV infection glutathione (GSH) content decreased, but glutathione disulfide (GSSG) levels increased. The quantitative GSH/GSSG ratio reflects the above changes of both peptides which, the more distinct, the greater the pathological changes in the uterine cervix.

Adolescent↗
PubMed1997

Folate deficiency and cervical intraepithelial neoplasia.

The presence of HPV, using the Digene Hybrid Capture System, was identified in a group of 324 women with CIN and in 228 women with normal cytological smears. Risk of occurrence of CIN was 40 times higher for high risk HPV types. The serum folic acid level and the level of antioxidant compounds in plasma (retinol, alpha-tocopherol, vitamins C and E) in women of the studied and control group was determined by HPLC (high-performance liquid chromatography-reversed phase). Statistically lower levels of folic acid were found in the women with CIN-HPV (+) (OR: 7.5: 95% CI: 1.2-9.7). Studies have shown that lower levels of antioxidants coexisting with low levels of folic acid increases the risk of CIN development.

Antioxidants↗
PubMed1997

[Use of the Digene Hybrid Capture System for identification of the human papillomavirus (HPV-Human Papillomavirus) in women with cervical dysplasia].

The study was performed to assess prevalence of HPV-DNA infections using the Hybrid Capture System (Vira Papl Vira Typ ). In a group of 228 female patients with Papanicolaou smear grade I and II, high oncogenic risk HPV infections were found in the 15.78% and low oncogenic risk--in 7.01% of the investigated patients. In a group of 324 women with diagnosed CIN, the high oncogenic HPV infection were found in 70.4% of them (CIN I/II-60%, CIN III-87.11%), and the low oncogenic risk HPV infections in 11.1%.

DNA, Viral↗
PubMed1996

Content of beta-carotene in blood serum of human papillomavirus infected women with cervical dysplasias.

Studies were carried out in 528 women hospitalized in the Department of Obstetrics and Gynecology Medical Academy in Lublin. Besides the control group, patients were classified according to the observed histopathological changes in the cervix (CIN) and found infections with human papillomavirus (HPV). In all cases beta-carotene content in blood serum was examined. HPV infection was probably a cause of decrease of beta-carotene content. It was found that with increased advancement of cervical dysplasia the level of beta-carotene in serum decreased.

Adolescent↗
PubMed1996

[Frequency of HPV infection and the level of ascorbic acid in serum of women with cervix dysplasia].

The effect of HPV infection on cervix dysplasia development and ascorbic acid level in 528 women was studied. HPV-DNA was estimated using Digene Hybride Capture System and ascorbic acid by color metric method with 2,4-dinitrophenylhydrazine at 520 nm. HPV infection can be connected with the risk of cervical pathology and cervical cancer. Besides, we observed lower level of ascorbic acid in groups of women with CIN, Ca in situ and HPV infected.

Adult↗
PubMed1995

[Salpingitis and tubal pregnancy in materials from the Clinics of the Surgical Gynecological Academy of Medicine in Lublin in the years 1978-1988].

This retrospective study was based on hospital records analysis. The incidence of tubal pregnancy and salpingitis were evaluated and possible correlation between these two diseases was estimated. Despite of many other authors reports about increase of tubal pregnancy incidence we observed decline of tubal pregnancy incidence in studied period. Increase of salpingitis incidence did not alter this trend. Authors conclude that recently reported increase of tubal pregnancy incidence in many countries is valid only in populations studied and can not be generalized. The increase of salpingitis incidence does not necessarily influence tubal pregnancy incidence.

Female↗
PubMed1995

[Frequency of HPV infection and level of glutathione in serum of women with cervix dysplasia].

We have investigated the effect of HPV infection on the development of cervix dysplasias and GSH level in the plasma of 312 women, ranging from 18 to 34 years (x = 23.2). HPV-DNA was estimated using the Digene Hybride Capture System and GSH by the high performance liqvid chromatography method (HPLC). HPV infectior can be connected with a risk of cervical pathology and cervical cancer. Besides HPV infection the other risk factor is a low level of one of the most important antioxidant-glutathione (GSH). The level of GSH decreased while the concentration of GSSG increased in the plasma of women with HPV infection, cervix dysplasias and cancer. Simultaneous investigation of biochemical and virologic parameters in conjugation with the known cytopathologic and epidemiologic risk variables can show the factors that may inhibit or promote cervical carcinogenesis.

Adolescent↗
PubMed1994

[Quantitative studies of urine sediment morphology during the course of pregnancy].

Bacterial infections of the urinary system during pregnancy occur relatively frequently and exert unfavourable effect on its course while their constant and sensitive indicators are leucocyturia and bacteriuria. The studies were carried out in 30 healthy women in the second phase of menstrual cycle and in 86 pregnant women in various stages of pregnancy (I, II and III trimester). The subject of own analysis was leucocyte count in 1 ml of morning urine portion in correlation with the result of quantitative and qualitative urine culture. As significant bacteriuria 10(4) bacteria in 1 ml of urine was accepted. Urine sediment examination was carried out by the Mc Geachie-Kennedy method. The own studies demonstrated an increase of morphotic element count in 1 ml of urine with the progression of pregnancy. This increase was statistically significant in the group of pregnant women with significant bacteriuria. The results of own studies were compared with those of other authors.

Adult↗
PubMed1994

[Salpingitis and tubal pregnancy in patients treated at the first Clinic of Surgical Gynecology in Lublin between the years 1978-1988].

Retrospective study was performed upon incidence of tubal pregnancy and histopathologically proved post-infection changes in oviducts. The incidence of tubal pregnancy was significantly decreasing in studied period in our institution despite the fact that salpingitis was found more often. Authors conclude that recently reported increase of tubal pregnancy is valid only in the populations studied and not in general. Relation between salpingitis and tubal pregnancy is not always evident.

Female↗
PubMed1994

[Comparison of routine semen analysis with results of the hypo-osmotic test and hamster penetration test].

In the program of semen preparation to intra uterine insemination (IUI) the comparison of the routine semen analysis with the results of the Hypoosmotic Test (HOS-T) and the Hamster Oocyte Penetration Test (HOP-T) was performed. The positive correlation between the sperm quality and the results of the used tests (HOP and HOS) was revealed. It was found that the essential indicator of the decreased fertilizing capacity of the semen is it's motility. The significance of the studied tests in the diagnosis and treatment of infertility was discussed.

Animals↗
PubMed1994

[Intrauterine insemination as a method of treating male infertility].

One of the method of treatment of the infertile couple due to the male factor is the Intra Uterine Insemination (IUI). The references on the subject are divergent. This is caused by the lack of the similar, comparable classification in patients selection for the treatment, procedure of semen preparation and the results estimation. The different efficiency of this treatment method and it's direct and indirect costs are emphasized. In our studies IUI was applied in 42 women from the couples infertile due to the male factor. There were 3 pregnancies obtained (7.0%) which gives the result of 4.3% of pregnancies respectively per treated cycle. Each stage of the procedure was analysed, beginning from the criteria of patients selection, the way of the semen preparation as well as the time of treatment in respect to the obtained results--the percentage of the patients who became pregnant.

Female↗
PubMed1992

[The value of ultrasound examination for diagnosing postmenopausal metrorrhagia].

The results of transvaginal ultrasound diagnostic assessment of endometrium were compared with histopathological examination of endometrium by following diagnostic abrasion on examined group of 54 postmenopausal women. The method seems to be useful for diagnosis of postmenopausal metrorrhagia, when the "safe" thickness of endometrium layer is taken as 5 mm. It occurs in effect of our study the transvaginal ultrasound examination may decrease the number of subsequent diagnostic abrasions in those cases.

Aged↗
PubMed1987

The effects of beta-adrenergic drugs on the human sperm motility in vitro. I. The effects of propranolol and isoprenaline.

The investigations were carried out on 28 samples of human semen with asthenozoospermia. The experiments were performed in three groups of semen, adding to the first one 0.34 mM of propranolol and 0.025 mM of isoprenaline, to the second one the double dose and to the last one the fivefold dose of the drugs. The lowest concentrations of the drugs applied in our investigations result from the used therapeutic intravenous doses for people. The proportional content of moving sperma was evaluated under the phase-contrast microscope at the 5, 30, 60, 120 and 240 minute of the experiment. The addition of 0.34 mM of propranolol caused a decrease of the sperm motility but only after 60 minutes and for a short period of time. However, the addition of the double and the fivefold doses of the drug led to immediate and significant reduction of the sperm motility. The lowest dose of isoprenaline markedly increased the sperm activity in comparison with the control values but not earlier than 120 minutes after the addition. The further increase in dose showed no effect on the moving sperm percentage increase, merely accelerating this effect.

Adult↗