Search PubMed⌕ Search

Biomedical subjects

M Sela

Publications and source records attributed to M Sela.

At least 289 records · Page 16Linked to original sources
PubMed1972

Thymus-independence of slowly metabolized immunogens.

The role of thymus in antibody responses to a series of four synthetic polypeptide immunogens of the general formula multi-copoly(Tyr,Glu)-poly(Pro)-poly(Lys) was investigated as a function of the optical activity of the amino acids composing their structure. Irradiated nonthymectomized and thymectomized SJL mice were injected with thymocytes, marrow cells, or a mixture of both. Each group of recipients was immunized with the following copolymer enantiomorphs: all L-amino acids; L-amino acids outside and D inside; D-amino acids outside and L inside; or all D-amino acids. The antibody response to the immunogen composed of all L-amino acids was thymus-dependent, whereas the responses to the other three copolymers were all independent of the thymus. Similar cell transfers were performed in DBA/1 mice immunized with multi-copoly(L-Phe,L-Glu)-poly(D-Pro)-poly(D-Lys). This mouse strain produces specific antibodies against the (Phe,Glu) region and against the poly(D-prolyl) region. The immune response to the determinant with only L-amino acids on the outside was thymusdependent, whereas the response to the inside immunopotent region with only D-amino acids was thymusindependent. Since earlier studies have demonstrated that synthetic polypeptide antigens that contain D-amino acids are poorly metabolized, the thymus-independence of the antibody responses to these multichain synthetic polypeptides that possess repeating antigenic determinants was correlated with the metabolizability of the immunogens or their component determinants.

Animals↗
PubMed1971

Stimulation in vitro with protein carrier of antibodies against a hapten.

Rabbits were immunized both with lysozyme and with dinitrophenylated bovine serum albumin. No antibodies against the dinitrophenyl hapten were produced when cell suspensions of their spleens were exposed in vitro to dinitro-phenyl-ovalbumin, whereas a positive response was obtained with dinitrophenyl-lysozyme. Moreover, when spleen cells from rabbits previously immunized with dinitrophenyl-bovine serum albumin were exposed in vitro to bovine serum albumin alone, they produced antibodies against the dinitrophenyl hapten.

Aminocaproates↗