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Biomedical subjects

M Seger

Publications and source records attributed to M Seger.

24 records · Page 2Linked to original sources
PubMed1984

Macrophage microtubules: an optimized method for the assay of tubulin concentration and state of polymerization in macrophages.

We describe a method for the assay of total tubulin content and the tubulin dimer/polymer ratio in cultured macrophages (MPs). The assay is based on the specific binding of [3H]colchicine to tubulin dimer units and on the separation of free and tubulin-complexed [3H]colchicine on diethylaminoethyl (DEAE)-cellulose filters. The native state of microtubules (MTs) was conserved by suspending the cells in a microtubule-stabilizing solution specifically adapted for use with MPs and by disrupting the cells under carefully chosen conditions. Intact MTs were isolated from the cell homogenate by centrifugation at 150,000 X g and were subsequently depolymerized. [3H]Colchicine binding assays were performed on both the supernatant fraction, containing the pool of soluble tubulin, and on the deploymerized MTs. By using this method, we examined the influence of low temperature and of a number of agents known to affect MP function, on the tubulin dimer/polymer ratio in guinea pig peritoneal MPs. The results of the biochemical assay correlated well with the published information, derived by morphologic and functional approaches, on the effect of cold and of these agents on MTs in phagocytic cells.

Animals↗
PubMed1982

Intrahepatic portal vein sclerosis in patients without a history of liver disease. An autopsy study.

Portal fibrosis with portal venous obliteration is characteristic of noncirrhotic portal hypertension, but similar lesions are also commonly seen in patients without clinical evidence of liver disease. Thus, the ability to predict the presence of portal hypertension with histologic criteria will probably depend on a quantitative assessment of liver tissue. The purpose of this study is to provide a quantitative basis in a "normal" population for evaluation of portal vein obliteration. We reviewed 414 consecutive autopsies of patients without known history of alcoholism or clinical liver disease. Intrahepatic portal vein obliteration was graded 0 to III. The grading system was standardized by morphometry on 34 selected cases. The incidence of portal vein lesions increased with age and reached a plateau at about 60 years of age. Lesions were more common in patients who had severe congestive heart failure or arterial thrombosis. These associations suggest that obliterative lesions may be the result of thrombosis in patients with sluggish portal blood flow or hypercoagulability. Portal tract mineral oil deposits may also have a role, because they were found more often than expected in livers with portal sclerosis.

Adult↗
PubMed1976

Neuron culture from adult goldfish.

Neurons and glia from the central nervous system of the adult teleost Carassius auratus have been grown as explant cultures of minced brain tissue and as trypsin dissociated cells. These cultures exhibit extensive neurite growth from two neuronal types, have organotypic ultrastructure, and contain electrically active cells. Autoradiographic data indicate that these neurons do not divide in culture, and histological evidence suggests that some mature neurons survive explantation and regenerate processes. However, explantation of brain fragments not containing undifferentiated cells, localized in the ventricular and subventricular zones in the brains of fish, resulted in mesenchymal and glial cell cultures only. Therefore, a contribution to the population of cells in culture by undifferentiated cells must be considered. The cultured neurons remained viable for at least 19 weeks and ultrastructural and electrophysiological data indicate synaptic interaction between cells in explant cultures.

Action Potentials↗
PubMedDate not supplied

Trauma outcomes: a death analysis study.

Survival and mortality outcomes for trauma patients admitted to Liverpool Hospital, Sydney were analysed to determine the adequacy of trauma care. TRISS and ASCOT survival probabilities and peer review were utilised to determine if deaths were avoidable. Evaluation methods were compared for assessment of care. During the study period 2205 trauma patients were admitted, 518 of which fulfilled the study entry criteria. There were 38 deaths. The age and Injury Severity Score (ISS) of survivors was 34 +/- 18 years, 9.8 +/- 9 (mean +/- sd) compared to age and ISS for nonsurvivors 37 +/- 22 years and 45 +/- 22*, *p < 0.001. Peer review suggested that 32 deaths were non avoidable, 4 potentially avoidable and 2 were probably avoidable. TRISS and ASCOT survival probabilities were > 0.5 in 16 and 18 patients respectively. TRISS and ASCOT had low positive predictive value (25%) in identifying avoidable deaths. The Z Score was 1.79. The standardised mortality ratio (SMR) was 1.16. The Effectiveness (E) value for outcome was 0.91. Poor communication within the Area Trauma System was the greatest contributor to avoidable deaths. All trauma deaths need peer review rather than solely relying upon ASCOT and TRISS probabilities to identify "unexpected" deaths for detailed review.

Adolescent↗