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Biomedical subjects

M Segall

Publications and source records attributed to M Segall.

At least 73 records · Page 4Linked to original sources
PubMed1976

Pooled stimulating cells as a "standard stimulator" in mixed lymphocyte culture.

Lymphocytes from normal unrelated donors were pooled and used as stimulating cells in mixed lymphocyte culture (MLC). Different pools, each consisting of cells from 20 or 30 different donors, stimulated approximately the same amount of 3H-thymidine incorporation by a given responder, and this "plateau level" of incorporation was different for different responders. Stimulation by pools of 20 cells was highly correlated with the general "responsiveness" of responding cells as measured by their mean response to a large panel of stimulating cells. Such pools may be useful as "standard stimulators" in quantitating MLC stimulation, and perhaps also in evaluating lymphocyte responsiveness of patients.

Bromodeoxyuridine↗
PubMed1975

Genetic and cellular control of in vitro models of allograft reactivity.

In each species sufficently studied, a single genetic region, the major histocompatibility complex (MHC), controls the strong transplantation antigens. Recent evidence suggests that the genetic control of differences important in allograft phenomenon is more complex than previously realized. In addition to the two loci, alleles of which control serologically defined (SD) antigens, there are other loci the phenotypic products of which lead to T-lymphocyte activation in mixed leukocyte culture. These latter loci have been referred to as LD (or lymphocyte defined). There appears to be a physiologic interaction between these two loci in that the LD differences seem to be important in the initial recognitive phases of the allograft reaction; the SD differences (or products of genes very closely linked to those determining the SD antigens) are important as a target for cytotoxicity as studied by the cell-mediated lympholysis test.

Animals↗
PubMed1973

Cell mediated immunity: separation of cells involved in recognitive and destructive phases.

The mixed leukocyte culture (MLC) and the cell mediated lympholysis (CML) assays are used as in vitro models of the afferent, or recognitive, and efferent, or destructive, phases of the homograft reaction. Activity in both of these tests has been related to differences at the major histocompatibility complex, HL-A in man and H-2 in mouse. Recent evidence suggests that the presumed cell surface differences which lead to cell proliferation in MLC are different from those which act as a target for CML. Data are presented providing further support for this hypothesis; in addition separate cell populations may respond to the differences which activate cells in MLC and to the differences which serve as targets for CML. There thus appears to be a dichotomy both for genetic control of, and cell populations involved in, the recognitive and destructive phases of cell mediated immunity.

Animals↗
PubMed1972

Genetic and immunological complexity of major histocompatibility regions.

There are genetic differences within the major histocompatibility complex of the mouse which lead to skin graft rejection but which cannot be detected serologically. When confronted with these differences on allogeneic cells, lymphocytes proliferate in vitro. In other cases, in vitro lymphocyte proliferation but no skin graft rejection is associated with loci that are linked to but genetically separable from the loci controlling the serologically defined antigens.

Alleles↗