[Serological identification and differentiation of strains of the Candida albicans species].
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Biomedical subjects
Publications and source records attributed to M Sefer.
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Three laboratory methods -- immunofluorescence, coagglutination and urinary immunoglobulin determinations -- were applied in 16 patients with urinary infections, of whom 12 with pyelonephritis and 4 with cystitis. There was an 100% agreement between the three tests in the pyelonephritis cases. In the cystitis cases, there was 100% agreement between immunofluorescence and urinary immunoglobulin and 50% between the three methods. Urinary immunoglobulin determination and coagglutination may be recommended as routine auxiliary clinical laboratory methods for early detection of renal lesions and localization of the infection.
The sensitivity of fowl leptospirae to antibiotics was tested by diffusiometry on solid media in Petri dishes. The method includes two stages: 1) visible leptospirae cultures are obtained by central seeding and incubation during 3--5 days at 28 or 37 degrees C; 2) antibiotic tablets are disposed at 2 cm from the visible edge of the multiplication zone of the leptospirae. The edge is marked with a pencil at the moment in which the antibiotics are applied. Incubation is continued at the same temperatures. The results are read after five days taking as reference the position of the marginal multiplication line. The sensitivity of leptospirae to antibiotics is expressed by arrested multiplication and resistance by advance of the marginal multiplication line up to or even beyond the antibiotic tablets. Fowl leptospirae are sensitive to penicillin, tetracyclin, chloramphenicol, streptomycin, novobiocin, polymixin, pristinamycin, neomycin, erythromycin, and resistant to mycostatin, septrin, rifampicin, optoquine, sulphatiazol.
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Microorganisms of the Mycoplasma genus were found in the washing fluids of the oral cavity in 2% of healthy youths, and in 57.1% of patients with parodontal lesions: 25.7% in the buccal fluid, 17.1% in the dental tartar and 34.2% in the gingiva exsudate. Microplasma was not found in dental caries and in the radicular canals with pulpar gangrene. Although these microorganisms were frequently found in subjects with parodontal lesions they cannot be implicated with certainty in their aetiology.
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