Search PubMed⌕ Search

Biomedical subjects

M Schweiger

Publications and source records attributed to M Schweiger.

At least 163 records · Page 9Linked to original sources
PubMed1980

Hereditary deficiency of adenine phosphoribosyl transferase.

We describe a 12 year old patient who developed acute renal failure at the age of 18 months due to radiolucent "uric acid" stones. Measurement of APRT activity showed a complete dificiency in this patient (less than 0.02%). In the urine we found instead of uric acid 2.8-dihyroxyadenine as measured by high pressure liquid chromatography. The pedigree indicates an autosomal-recessive inheritance of this disorder.

Acute Kidney Injury↗
PubMed1979

Elastic stockings in the control of hand oedema.

Elastic support and compression in the form of an elastic finger stall or glove seems to be a simple and comfortable way to obviate and reduce oedema of the hand and fingers after surgery or trauma. It may also prove useful in inflammatory conditions such as rheumatoid arthritis, though the authors have not used it as yet for that condition.

Bandages↗
PubMed1979

Radiation sensitivity of messenger RNA.

Messenger RNA function is inactivated by irradiation with ultraviolet light. A unit length mRNA (in bases) is 2-3 times more sensitive than a unit length of DNA (in base pairs) with respect to the inactivation of template function. These data stem from four experimental systems all of which do not repair DNA: the translation of E. coli mRNA in rifampicin-treated cells, of T7 mRNA in infected E. coli, of f2 phage RNA in vivo, and of stable mRNA in chromosomeless minicells. The comparison of relative sensitivities to UV is relevant to the technique of UV mapping of transcription units which enjoys increasing popularity in pro- and eukaryotic genetic research.

DNA, Bacterial↗
PubMed1979

Method for determining individual contributions of voluntary and involuntary anal sphincters to resting tone.

With simultaneous recordings of the external anal sphincter EMG activity and the anal resting pressure, it is possible to estimate the isolated part played by the internal anal sphincter on the resting pressure by means of a correlation calculation with extrapolation to zero EMG amplitude. The value of the method was proven by comparative investigations in conscious and curarized patients under general anesthesia. In a group of 20 normal people we found that the part the internal sphincter has in the total resting pressure was 75 per cent. Because of the very low standard deviation of +/- 5.5 per cent the method is superior to others for comparing various anal disorders.

Anal Canal↗
PubMed1979

Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane.

The phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli is inhibited by the energized of the membrane. This was shown in intact cells as well as in membrane vesicles. Relaxation of the proton gradient by uncouplers stimulated the uptake of sugars via the phosphotransferase system in aerobically cultured cells. No such effect was seen in anaerobic cells, apparently because the cell membrane of these cells is poorly energized. Energization by respiration of D-lactate or ascorbate inhibited the phosphotransferase uptake system in membrane vesicles. This inhibition was reversed by the addition of cyanide. Oxamate, a specific inhibitor of lactate dehydrogenase, prevented the inhibitory effect of D-lactate. Membrane vesicles prepared from a cytochrome-less mutant were not energized by D-lactate oxidation and the phosphotransferase uptake system was not inhibited.

Biological Transport, Active↗
PubMed1979

Is the DNA of virus T7 methylated?

DNA modification of T7 wild type and of T7 M-mutants was studied by determining the percentage of 5-methylcytosine (5MC)/cytosine (C) and N6-aminopurine (6MA)/adenine (A) and by evaluating the plating efficiencies of restriction-sensitive T7 M-mutants on modifying and non-modifying host strains. Only 0.03% adenine and 0.02% cytosine were methylated in the DNA of T7 wild type as well as in T7 M-mutants, which was independent of the host DNA methylation (30- to 50-fold higher). The restriction of T7 M-mutants, determined from the plating efficiencies, was not altered on modifying or non-modifying hosts. These results indicate that host-specific modification is blocked during T7 development and that this is not due to the M-protein.

Adenine↗
PubMed1979

A novel ATP-driven glucose transport system in Escherichia coli.

In Escherichia coli wild-type cells and in ATPase-deficient cells (unc mutants), glucose was found to be transported mainly by an ATP-driven system. The evidence is based on experiments involving interference at different sites of energy metabolism with the use of uncouplers, arsenate, and starved cells. Furthermore, addition of succinate to starved cells increased glucose uptake only in the wild-type cells, where ATP could be regenerated. Glucose transport was also ATP-dependent in cells deficient in methyl-beta-galactoside transport (a system that carries glucose specificity). It was found to be shock-sensitive in all strains tested. The NOVEL ATP-driven glucose transport is a high-affinity (Km 3-10 microM) and high-capacity (V 240-330 Mmol . min-1 . mg cell protein-1) uptake system.

Adenosine Triphosphate↗
PubMed1979

Development of Escherichia coli virus T1: escape from host restriction.

The bacterial virus T1 grows interchangeably on different Escherichia coli strains (C, B, and K). This implies that T1 has an efficient mechanism to overcome the host restriction barrier. The DNA of T1 was found to be methylated independently of the hosts. The percentage of N6-methyladenine varied from 1.6 to 1.8, and the 5-methylcytosine content varied from 0.1 to 0.4%. In contrast, the range in percentage of N6-methyladenine and 5-methylcytosine found in the hosts was 0.7 to 2.4 and 0.0 to 1.1, respectively.

Coliphages↗
PubMed1979

[A method for differentiation between the participation of the smooth and the cross-striated anal sphincter muscles in resting tonus].

By means of simultaneous recordings of the external anal sphincter EMG activity and the anal resting pressure with at least ten voluntary sphincter contractions, it is possible to estimate by correlation calculation the resting pressure exerted by the internal anal sphincter. The value of the method had been proved by comparing investigations in conscious and curarized patients under general anesthesia. In patients with hemorrhoids and chronic constipation, the amount of internal pressure found in the resting tonus (91% in hemorrhoids, 55% in chronic constipation) was significantly different when compared with a health group. The comparison of only the resting pressure did not show a significant difference.

Anal Canal↗
PubMed1978

Deficiency of DNA ligase activity in Fanconi's anemia.

A significant decrease in DNA ligase activity was observed in lymphocytes and fibroblasts of a patient with Fanconi's anemia (FA). This decrease is related to the observed DNA repair deficiency indicated by the delayed closing of repair DNA strands following UV irradiation. Other steps of DNA repair were analyzed in the FA fibroblasts, including endonucleolytic incision of DNA, repair DNA synthesis, and exonucleolytic removal of the photoproducts. No differences were found against control cells. The action of DNA ligase is delayed during replication in the FA cells, as seen by an accumulation of replicative intermediates.

Anemia, Aplastic↗
PubMed1978

[Primary healing of the sacral cavity after proctectomy - a solvable surgical problem (author's transl)].

Primary healing of the perineal wound and the sacral cavity after a.p. resection of the rectum is an old surgical problem. In order to achieve primary healing we are using a lengthened omental graft to fill up the sacral space. Since January 1977 to July 1977 we used this method in 17 patients. 15 patients had very low rectal cancers. 2 patients had to undergo proctocolectomy because of ulcerative colitis. In 14 cases we achieved primary healing, among these the colitis patients. Overall we had three abscess formations in the sacral cavity. One of these patients got in addition an obstruction postoperatively.

Abscess↗
PubMed1978

Nitrofurantoin damages DNA of human cells.

Nitrofurantoin causes damage to DNA of cultured diploid human fibroblasts. As a consequence DNA synthesis is blocked. The damage is removed by the normal enzymatic DNA repair system. Xeroderma pigmentosum fibroblasts which are defective in the excision endonuclease fail to repair nitrofurantoin-caused lesions.

DNA↗