Search PubMed⌕ Search

Biomedical subjects

M Schultze

Publications and source records attributed to M Schultze.

At least 37 records · Page 2Linked to original sources
PubMed1990

Open reading frame VIII is not required for viability of cauliflower mosaic virus.

Open reading frame (ORF) VIII of cauliflower mosaic virus (CaMV) was analyzed by site-directed mutagenesis in order to investigate its potential function for the viral life cycle. Removal of either the start or the stop codon of ORF VIII, as well as interruption of ORF VIII by a new stop codon, did not affect infectivity. Unlike certain ORF VII mutants all three ORF VIII mutants are stable. Hence the ORF VIII product is not essential and ORF VIII mutations do not have deleterious polar effects on the expression of the downstream ORF V, which codes for the viral protease/reverse transcriptase.

Codon↗
PubMed1989

The expression, localization, and effect of a human interferon in plants.

The ORF II of Cauliflower mosaic virus (CaMV) DNA was replaced with the human IFN alpha D coding sequence to yield a stable CaMV strain designated Ca524i. Inoculation of turnip (Brassica rapa cv "Just Right") with strain Ca524i DNA excised from plasmid pCa524i resulted in the production of biologically active IFN alpha D in infected plants. This was also true for its mutant (Ca562i) where one of the CYS codons was deleted. IFN alpha D produced in planta did not hamper superinfection with a single-stranded (+) sense RNA plant virus, turnip yellow mosaic virus (TYMV). Antibody gold labeling techniques and electron microscopy of infected plants showed that IFN was localized in the CaMV inclusion bodies.

Cell Compartmentation↗
PubMed1988

[Direct biophysical effects of ultrasonic stimulation on the living rabbit lower limb].

Ultrasonic applications of low up to high intensity during 2 minutes led to biophysically provable changes (behaviour of bioimpedance, pO2, and temperature) in vital tissues (musculature, periosteum, and bone marrow). The most effective application was noticed by static ultrasonic therapy of 0.2 W/cm2. Repeated ultrasonic therapies of low intensity effected involved, for instance, an adaptation of the temperature and an increase of the electrophysical stimulation (bioimpedance).

Animals↗