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Biomedical subjects

M Sauter

Publications and source records attributed to M Sauter.

63 records · Page 4Linked to original sources
PubMed1987

Characterization of an Epstein-Barr virus nuclear antigen 2 variant (EBNA 2B) by specific sera.

The Jijoye Epstein-Barr virus (EBV) strain is characterized by a substitution of 1.8 kb in the C-terminal part of EBNA 2 gene compared to B95-8 or M-ABA virus. Protein immunoblot analysis using human sera against EBNA 2 indicated that an immunological variant to the EBNA 2 of B95-8 (type A) is encoded by the Jijoye virus (type B). In order to generate a specific EBNA 2B antiserum the NaeI/NsiI DNA fragment of the Jijoye virus containing 237 bp of the C-terminus from the EBNA 2B gene was cloned in an E. coli expression vector (pME3). The resulting fusion protein contained 79 C-terminal amino acids of the viral protein and a 37,000 Da part of the bacterial anthranilate synthase. Rabbit antisera generated against this fusion protein reacted specifically with two proteins of 73,000 and 77,000 Da from Jijoye cells and three other cell lines carrying type B virus, while no proteins could be identified in the type B cell line BL 29. In addition, using these sera directed against the pME3 fusion protein, no reaction could be observed with the EBNA 2A protein from the B95-8 and several other cell lines containing type A virus.

Antigenic Variation↗
PubMed1985

Identification of the coding region for a second Epstein-Barr virus nuclear antigen (EBNA 2) by transfection of cloned DNA fragments.

Cell lines were established by co-transfection of cloned M-ABA Epstein-Barr virus (EBV) DNA fragments with plasmids conferring resistance to dominant selective markers. A baby hamster kidney cell line carrying the HindIII-I1 fragment exhibits a nuclear antigen of 82 000 daltons, serologically defined as EBV-determined nuclear antigen (EBNA) 1. Furthermore, a Rat-1 cell line transfected with DNA of the clone pM 780-28 containing three large internal repeats (BglII-U) and the adjacent BglII-C fragment expresses a nuclear antigen of 82 000 daltons which can be visualized only by a subset of anti EBNA-positive human sera. Sera recognizing the 82 000-dalton protein of the transfected cell line reacted with a protein of the same size in the non-producer line Raji, designated as EBNA 2. Conversely, sera without reactivity to the 82 000-dalton protein failed to react with EBNA 2 of Raji cells. P3HR-1 and Daudi cells with large deletions in BglII-U and -C are devoid of EBNA 2. The data presented provide evidence that a second EBNA protein is encoded by the region of the EBV genome which is deleted in the non-transforming P3HR-1 strain.

Antigens, Viral↗
PubMed1976

Xerophthalmia and measles in Kenya.

In many African countries measles is considered to be an important cause of blindness. On the basis of his observations in Kenya and Tanzania in 1972 Franken presumed, however, that in the majority of these cases xerophthalmia was the real cause of blindness, precipitated by the "catalyst" measles. In order to gain a better understanding of this important complicated problem, we performed in the first half of 1974 an investigation in Kenya into the prevalence of xerophthalmia. In December 1974 we had the opportunity to evaluate our Kenyan findings on Java, in the company of Dr. J. ten Doesschate and Professor H.A.P.C. Oomen. The results of this investigation in Kenya and Indonesia are presented in this thesis. (see article) 1. Xerophthalmia occurred nearly everywhere in Kenya in 1974. This demonstrates the prevalence of xerophthalmia in communities which - do not have rice but - have maize for their staplefood. 2. Xerophthalmia appears to be the main cause of blindness in Kenyan children. 3. Measles often plays - by means of local and general "catalysing" effects - an important role in the development of blindness caused by xerophthalmia. 4. In well-nourished children measles is of no consequence as a cause of blindness. 5. Vital staining by 1% rose bengal or 1% lissamine green appears to be a real asset for the early diagnosis of xerophthalmia in Health Centres and in field surveys. This method is therefore of great importance for the prevention of severe, blindness inducing vitamin A deficiency.

Adolescent↗
PubMedDate not supplied

Using the change process to implement nursing diagnoses.

The purpose of this study was to assess the impact of implementing nursing diagnosis in a community hospital where only 32.5% of medical-surgical patients had individualized care plans. Of these care plans, only 13% correctly identified North American Nursing Diagnosis Association (NANDA) nursing diagnoses. Prior to the study, staff lacked commitment toward written care plans and viewed time management as the major clinical barrier to completion. Staff were instructed in nursing process and nursing diagnosis through a mandatory continuing education series. A follow-up survey at 6 months revealed care plans completed according to NANDA standards in 82% of cases. The nursing staff verbalized an increased commitment and appreciation of nursing process and its influence on patient care.

Education, Nursing, Continuing↗
PubMedDate not supplied

[Prenatal hypoxic brain damage (prenatal CTG and postpartum CT changes].

A para III was found to have a constantly silent CTG. In two fetal blood analyses the pH was normal. During the first few hours post partum the infant had rapidly increasing neurologic disturbances with violent convulsions and coma. As early as on the first day of life the computer tomogram showed extensive, later persistent hypodense zones corresponding to severe asphyxial cerebral necrosis. Based an the course of CT changes it has to be assumed that the hypoxic crisis occurred some days prior to the onset of labor. Pathologic changes in the umbilical cord indicated that the cause could have been a transitory occlusion in utero. The computer tomogram enables cerebral insults to be dated more accurately. If prenatal hypoxia occurs repeatedly new methods of prevention must be sought.

Adult↗