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Biomedical subjects

M Satomi

Publications and source records attributed to M Satomi.

At least 55 records · Page 3Linked to original sources
PubMed1993

Increase in vertical dimension alters mechanical properties and isometric ATPase activity in guinea pig masseter.

To study the changes in mechanical and metabolic properties associated with an increase in the vertical dimension of the face, isometric tension, isometric ATPase activity, unloaded shortening velocity (Vmax), and the tension transients in response to step stretches in length were measured at constant levels of various Ca2+ activations in glycerinated masseter muscles (75 to 150 microns in diameter and about 3 mm long) from normal and bite opened (6 mm increase in the vertical dimension, period of 1 week) guinea pigs. The isometric tension increased sigmoidally with an increase in Ca2+ concentration in both preparations. However, the bite opening shifted the pCa-relative tension relationship in the direction of increasing Ca2+ required for activation, and the pCa at Km (Ca2+ concentration required to develop half maximum tension) was 6.40 +/- 0.08 in control and 6.27 +/- 0.05 in the bite opened preparations. The maximum isometric tension at the saturating Ca2+ concentration was greater in the bite opened preparation. The ATPase activity changed almost in parallel with the tension (i.e., Ca2+ concentration) in both preparations. However, the tension cost (ATPase activity/tension) was significantly (p < 0.05) lower in the bite opened preparation, compared with the control. The Vmax at the saturating Ca2+ concentration was lower in the bite opened preparations. The tension transients in response to step stretch in length were characterized by four distinct phases; the first phase of an immediate tension increase coincident with the stretch, the second phase of a rapid quasi-exponential tension reduction, the third phase of a minute tension increase and the fourth phase of a slow tension reduction.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenosine Triphosphatases↗
PubMed1991

[Supranuclear vertical gaze palsy and convergence nystagmus caused by unilateral riMLF lesion].

We report a case showing supranuclear vertical gaze palsy and convergence nystagmus caused by a unilateral lesion of the rostral interstitial nucleus of the medial longitudinal fasciculus (riMLF). The patient was a 54-year-old female with mitral stenosis and regurgitation and atrial fibrillation, who suddenly developed vertigo and double vision. She was admitted to our hospital because of persisting diplopia 4 days after onset, although vertigo had resolved within 1 hour. On admission she was alert, but presented with supranuclear vertical gaze palsy and convergence nystagmus. Other cranial nerves were intact and motor strength, deep tendon reflexes, sensations were also normal. There were no cerebellar signs. Cranial MRI demonstrated a unilateral ischemic lesion at the left thalamo-mesencephalic junction that involved the unilateral riMLF. Cerebral angiography revealed no abnormalities. Vertical gaze palsy has been reported to be caused by a lesion involving bilateral riMLF or unilateral posterior commissure, and convergence nystagmus usually by a lesion near or within the dorsal mesencephalon. However, recent reports have demonstrated a histopathologic evidence that vertical gaze palsy was caused by unilateral riMLF lesion. The present case confirms clinically that both vertical gaze palsy and convergence nystagmus can be developed by a lesion of unilateral riMLF.

Brain Diseases↗
PubMed1990

Difference in enzymatic sulfation of bile acids between the mouse and rat.

Species difference in bile acid-sulfotransferase (BAST) activity was studied between the mouse and rat. Cytosol fractions of the liver, kidney, small intestine and large intestine were incubated with bile acids and 3' phosphoadenosine-5'-phosphosulfate. The mouse liver showed BAST activity for lithocholic acid, taurolithocholic acid and taurochenodeoxycholic acid, whereas the rat liver and kidney had the activity for taurodeoxycholic acid in addition to these compounds. The highest activities were found in the mouse liver and rat kidney. The mouse small intestine showed weak activity only for lithocholic acid. No activity was found in other organs. BAST was inactive towards taurocholic acid, 7 alpha- or 12-monohydroxy-5 beta-cholanoic acid. Optimal pH of liver BAST in the two species was different from that of the rat kidney. BAST of the mouse liver showed the highest activity without addition of Mg2+, whereas that of the rat liver and kidney showed enhancement by exogenous Mg2+. These results indicated that the distribution and characteristics of mouse BAST was different from rat BAST. Such difference should be reminded in any animal study involving the two species.

Animals↗
PubMed1990

[Campylobacter pylori in patients with gastroduodenal disease].

Recent advances of Campylobacter pylori (C. pylori) in human gastric disease and peptic ulcer were reviewed. C. pylori is a microaerophilic, motile, gram negative spiral rod bacterium. And all strains of C. pylori has a strong urease activity. In our experience. 91% of duodenal ulcer, 88% of gastric ulcer and 43% of control have C. pylori associated gastric mucosa. Patients of peptic ulcer with C. pylori infection were high relapse than patients of peptic ulcer without C. pylori, C. pylori is now known to be the most common and important case of pathologic gastritis, and C. pylori infection have been associated with gastric ulcer, duodenal ulcer, and non-ulcer dyspepsia. Although it has been only possible to culture C. pylori for about 6 yr in Japan, there are already sufficient data available to allow us to develop the basic framework that relates C. pylori gastritis to the causation of peptic ulcer disease.

Campylobacter↗
PubMed1989

[Inverse ocular bobbing associated with cerebral embolism and diabetes insipidus--a case report].

Inverse ocular bobbing (IOB) is an uncommon abnormal eye movement. Its characteristics are slow downward eye movement with rapid upward return, a nadir at the extreme of downgaze position and horizontal roving eye movement. We present a case of IOB associated with cerebral embolism and diabetes insipidus. A 69 year-old right-handed woman was admitted because of a consciousness disturbance. She had been well until November 10, 1983, when she was found dysarthric and left hemiplegic. On admission, she was stuporous. There were conjugate deviation to the right, central left facial and hypoglossal palsy, left hemiplegia with spasticity, left hyperreflexia with positive pathologic reflex and anosognosia. A CT scan performed on November 11 showed extensive hypodense area in the region supplied by the right middle cerebral artery. A right carotid angiography revealed multiple occlusions in the top of the right internal carotid artery with poor collateral circulation. After admission, the level of consciousness gradually deteriorated and became comatose on November 18, when the following abnormal eye movements were observed. Following spontaneous horizontal roving eye movement, both eyes deviated downward slowly from midposition, taking 1 to 2 seconds to reach the nadir. The eyes then remained in the position for 1 to 15 seconds, followed by a rapid return to the midposition. These abnormal eye movements are compatible with inverse ocular bobbing (IOB) described by Knobler. Electronystagmography detected typical IOB and spontaneous upward nystagmus. There was no evidence of hypoxia when these abnormal eye movements were present.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1987

ATPase activity and tension development in mechanically-skinned feline jaw muscle.

At a muscle length L0 (just taut), isometric tension and ATPase activity were measured at constant levels of various Ca2+ activations in muscle fibres (2-3 mm long and 34-66 microns in diameter) isolated from temporal, masseter and digastric (anterior belly) muscles. The isometric tension increased in a sigmoid fashion with increasing Ca2+ concentration from about pCa 6.60 to pCa 5.00 in the temporalis and from about pCa 6.37 to pCa 5.00 in both the masseter and digastric. The maximum isometric tension from four preparations of each muscle averaged 44.4 +/- 6.6 g/mm2 in the temporalis, 31.9 +/- 4.0 g/mm2 in the masseter, and 23.9 +/- 5.5 g/mm2 in the digastric. The ATPase activity also increased sigmoidally with increasing Ca2+ concentration from slightly below pCa 7.0 to pCa 5.0 in the temporalis, and from slightly above pCa 6.0 to pCa 5.0 in both the masseter and digastric. The maximum ATPase activity obtained from four preparations of each muscle averaged 0.35 +/- 0.06 mumol/mg protein per min in the temporalis, 0.29 +/- 0.06 mumol/mg protein per min in the masseter, and 0.18 +/- 0.04 mumol/mg protein per min in the digastric. The tension cost (ATPase activity/tension) estimated from these results was lower in the digastric than in the temporalis or masseter, indicating more economical ATP consumption in the digastric.

Adenosine Triphosphatases↗
PubMed1981

Fecal bile acid analysis in healthy Japanese subjects using a lipophilic anion exchanger, capillary column gas chromatography and mass spectrometry.

Detailed fecal bile acid profiles of healthy Japanese subjects were studied using a lipophilic anion exchanger, capillary gas chromatography and mass spectrometry. Total dairy excretion of bile acid into feces corrected for by fecal markers were between 127.99 to 366.33 mumole per day. Unconjugated bile acids constituted a major part, between 80 and 96%, of fecal bile acids. Glycine conjugated, taurine conjugated and sulfated bile acids were between 1 to 6, 0 to 3 and 1 to 10%, respectively. Esterified bile acids at C-24 position existed between 1 to 5%. Primary bile acids ranged from 0 to 55%. There were a number of epimers of hydroxy- and keto-bile acids, and lithocholic and deoxycholic acid were major secondary bile acids among them. A cholenoic acid was detected in the unconjugated fraction of one subject. It seems necessary to analyze the details not only on the type of bile acids but also on the mode of conjugation in biological samples. Thus, the methodology described in this study has made it easier to investigate on the role of bile acid in the physiology or pathophysiology of the gastrointestinal tract.

Adult↗