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Biomedical subjects

M Sasa

Publications and source records attributed to M Sasa.

At least 253 records · Page 14Linked to original sources
PubMed1975

A successful method for mass culture of the house dust mite, Dermatophagoides pteronyssinus (Troussart, 1897).

Comparative studies have been carried out to find out an optimum condition for efficient mass culture of Dermatophagoides pteronyssinus (Troussart, 1897), the most dominant species of the house dust mites. Among various food materials tested for culture media, the mixture composed of 2 parts of powdered laboratory animal food, 2 parts of dried yeast and 1 part of dried fish powder was found to be most fitted. The humidity content of the medium was best when adjusted to 16% beforehand and thin layer of the food medium was prepared in Roux's-bottle with a paper plug. The plug of the bottle was opened once a week and the medium was mixed thoroughly by shaking to prevent clotting. When about 15,000 mites were inoculated into 100 g of culture media, the highest yield was obtained with relative humidity regulated at 75% and the temperature at 25 degrees C after 12 weeks of incubation. Purified mites were separated from culture media by floatation with saturated NaCl solution and the average yield of the mice was 2 to 3 g in wet weight per 100 g of the food mixture.

Animals↗
PubMed1974

Impariment by 6-hydroxydopamine of locus coeruleus-induced monosynaptic potential in the spinal trigeminal nucleus.

All experiments were performed on cats immobilized with gallamine. In the rostral part of spinal trigeminal nucleus (STN), single fiber action potential with a consistent and short latency was elicited by electrical stimulation of the locus coeruleus (LC). When 2 mg of 6-hydroxydopamine (6-OHDA) dissolved in 5% ascorbic acid solution was applied into the lateral ventricle, the STN field potential produced by LC stimulation was reduced in amplitude within 20 hr. The STN potential elicited by trigeminal nerve stimulation, however, was unaffected until 24 hr after 6-OHDA. The drug also blocked the inhibitory effect of LC conditioning stimulation on the STN potential elicited by trigeminal nerve stimulation, whereas it did not modify the inhibitory effect of conditioning stimulation of the sensory cortex on the STN potential. Ascorbic acid solution, a solvent, affected neither the STN potential by LC stimulation, nor the inhibitory effect of LC neurons on the STN potential elicited by trigeminal nerve stimulation. These results strongly suggest the existence of noradrenergic fiber from the LC to STN, through which inhibition of the STN neurons is produced.

Action Potentials↗