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Biomedical subjects

M Sargent

Publications and source records attributed to M Sargent.

50 records · Page 3Linked to original sources
PubMed1985

Ego development in college.

Using the Sentence Completion Test for ego development, we studied several cohorts of students between 1971 and 1979 at a technological institute (Tech) and between 1974 and 1979 at a predominantly liberal arts university (MU). Ego level tended to rise slightly except among women at MU, for whom there was a slight but consistent loss. This particular finding challenges one assumption of a widely accepted version of Piagetian theory: that stage development is irreversible. Women tended to enter MU slightly ahead of men in ego level, but left at the same level. Contrary to expectation, men and women appeared to gain more at Tech than at MU; the difference was significant only for women.

Age Factors↗
PubMed1971

Isolation, mapping, and characterization of trehalaseless mutants of Neurospora crassa.

Mutant strains of Neurospora crassa that lack trehalase and are unable to grow on trehalose were isolated, and the gene (tre) was positioned on the right arm of linkage group I. Maltase and beta-galactosidase activities are almost identical in tre(-) strains, whereas that of invertase was reduced by more than half and those of acid phosphatase and amylase were somewhat increased. Heterocaryons between standard and trehalaseless strains yield less than one-tenth the activity of the former. In addition, strains with duplications heterozygous for trehalase produce less than 1% of the activity of the standard strain. An inhibitor of trehalase has been found in tre(-) strains; its sensitivity to heat and proteolysis, and its nondialyzability suggest that this substance is a protein. The mig gene, which determines the rate of migration of trehalase on acrylamide gels, has been shown to be less than 1 map unit away from the tre gene.

Acid Phosphatase↗
PubMedDate not supplied

Behavioral management of the agitated head trauma client.

This article identifies practical application of behavior management for the agitated head-injured client (Rancho levels III-V). In addition to the suggestions offered in the article, family counseling and education modalities can be used to meet the unique needs of the agitated head-injured individual.

Behavior Therapy↗
PubMedDate not supplied

Postacute, home-based head injury rehabilitation: an outcome study.

A functional scale was developed to evaluate gains made by head-injured clients involved in an intensive home-based rehabilitation program. The scale was designed to assess achievement in areas of practical daily skills that can be correlated with rehabilitation outcome. This research project assessed functional changes in head-injured clients with the expectation of their being able to decrease their dependence on caretakers. This article discusses the development of a home-based rehabilitation program, the creation of the functional scale, and the results of the project.

Activities of Daily Living↗
PubMedDate not supplied

Tandem repeat polymorphism and heteroplasmy in the mitochondrial control region of redfishes (Sebastes: Scorpaenidae).

Three species of redfish (Sebastes) share a common pattern of mitochondrial DNA tandem repeat polymorphism and heteroplasmy in the northwest Atlantic Ocean. All three species exhibit 9-17 copies of an approximately 275 base pair (bp) tandem repeat situated within the 3' domain of the control region. Sequence analysis of cloned mtDNA from S. mentella revealed that the tandem array is adjacent to the tRNA(phe) gene, and that the repeat shares 53% identity with the tRNA(phe) gene and part of the 12S rRNA gene. These features, as well as potential secondary structure assumed by the repeat, are consistent with previously proposed models explaining tandem duplications in the 3' end of the control region. In a sample comprising 36 S. fasciatus, 52 S. mentella, and 13 S. marinus taken near Newfoundland, neither the mean number of repeats per fish (12.2-12.7) nor the frequency of heteroplasmy varied significantly among species. A total of 42% of the redfishes were heteroplasmic, bearing either two or three repeat variants (33% and 9%, respectively). The similarity of the frequency distributions of tandem repeat variants in the three species suggests either a common balance between mutation and selection in the three species, or mitochondrial gene flow between them.

Animals↗
PubMedDate not supplied

Normal cells and malignant cells transfected with the HRas oncogene have the same heat sensitivity in culture.

Mouse embryo cells (C3H 10T1/2) were transfected with a plasmid (pAL8A) containing the HRas oncogene and neomycin resistance gene. Five transfected cell clones were isolated and established as cell lines, and these showed neomycin resistance. Two of these cell lines expressed a normal morphology while three showed a transformed morphology. Four of the cell lines produced tumours in nude mice. Flow cytometry measurements showed that exponentially growing cell cultures of the five transfected cell lines had the same cell cycle distribution as the normal parental cell line. The sensitivity to hyperthermia of the five transfected cell lines was the same as that of the normal cell line for temperatures ranging from 42.0 to 45.0 degrees C. Thus in these studies we found no difference in the thermal sensitivity of normal and malignant cells transfected by the Hras oncogene.

Cell Line↗