Biomedical subjects
Publications and source records attributed to M Sano.
Dopamine beta-hydroxylase activity was higher in mesenteric vessels, adrenal glands, and serum of 3-week-old spontaneously hypertensive rats but lower in the locus coeruleus than it was in the control Wistar-Kyoto rats. The results support the concept that the nervous system is an important regulator of blood pressure.
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The detection of a small amount of 13C labelled methyl benzoate and its metabolites in human urine dosed 13C labelled aspirin was carried out by a new mass fragmentographic technique.
Acute serum sickness in the rabbit was studied with special reference to the role of monocytes in the inflammatory process in the glomerulus. It was revealed that macrophages were the major factor in producing glomerular hypercellularity in acute serum sickness. Proliferation of intrinsic glomerular cells or accumulation of polymorphonuclear leukocytes (PMNs) was minimal. Ultrastructural characteristics of these phagocytic cells were described. Macrophages engulfed various inflammatory products such as fibrin and cell debri in the glomerular capillary. Colloidal carbon administered at the active inflammatory stage was found to be mostly engulfed by macrophages, little by mesangial cells, and was not seen in endothelial or epithelial cells and PMNs. The selective ingestion of the carbon particles by these macrophages made it possible to differentiate them from glomerular cells. This in turn indicated that the macrophages were derived from neither endothelial nor menangial cells and that they were of blood monocytic origin. It was suggested that monocytic cells participated in glomerular inflammation but they, on the other hand, contributed to the repair of glomerular injuries through their active role for phagocytosis.
Histiological analysis was performed on the lesions of the coronary artery and myocardium in accelerated serum sickness of the rabbit. Angiitis was seen to occur on the arterioles in the myocardium and was characterized by fibrinoid necrosis of the media with monocyte accumulation. Myocarditis was observed to be rather prominent in the right ventricle, where monocyte accumulation was associated with scattered degeneration or necrosis of myofibrils. In both lesions, the localization of antigen could hardly be detected in the inflammatory foci, suggesting the irrelevancy between the development of the tissue damages and the permanent deposition of immune complexes. Monocytes were seen to participate in the repair of the tissue injury.
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Guanosine 3':5'-monophosphate(cyclic GMP)-dependent protein kinase which catalyzes the phosphorylation of histone was purified about 200-fold from the soluble fraction of pig lung by pH 5.5 precipitation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. The apparent Ka values for guanosine 3':5'-monophosphate and adenosine 3':5'-monophosphate were determined to be about 17 and 360 nM, respectively. Mg2+ was essential for the activity exhibiting biphasic stimulation behavior and neither Mn2+ nor Ca2+ could substitute for Mg2+. However, these divalent ions markedly inhibited the protein kinase activity stimulated by cyclic GMP in the presence of Mg2+.
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To analyze the early stages of argyrophil cell carcinoid growth, complete serial sections were cut from the glandular portion of the stomachs of 22 Mastomys and submitted to Sevier-Munger's reaction. The 154 grossly invisible foci of argyrophil cell microproliferation thus detected were classified into three stages of microproliferations (I, II, and III), and the last stage was definitely a microcarcinoid. There was a gradual transition in cell proliferation among these three stages; the first stage (microproliferation I), in which the cells were morphologically indistinguishable from those of hyperplastic proliferation by general morphologic criteria, was where the initial change of argyrophil cell carcinoid formation was detectable by a light microscope. Whereas multiple occurrences of microcarcinoids accounted for the multiplicity of well-developed tumors in the stomachs of Mastomys, a well-developed carcinoid in this species was formed by the confluence of several microcarcinoids.