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Biomedical subjects

M Sano

Publications and source records attributed to M Sano.

At least 595 records · Page 33Linked to original sources
PubMed1985

[Effects of a new vasodilator, budralazine on water drinking activity, plasma norepinephrine, plasma angiotensin II, plasma arginine vasopressin, plasma serotonin concentration, urinary aldosterone excretion rate and urinary catecholamine excretion rate in rats].

Present study was undertaken to elucidate the effects of a new vasodilatating antihypertensive drug, budralazine on water drinking behavior and humoral factors including plasma norepinephrine (NE), angiotensin II (A II), arginine vasopressin (AVP), serotonin (5-HT) concentrations, urinary aldosterone and catecholamine excretion rates. After oral budralazine administration (10 mg/kg/day, p.o., for 7 days), systolic tail blood pressure of Wistar Kyoto rats (WKY) decreased significantly. While, heart rate and water drinking activity of WKY significantly increased. Urinary catecholamine excretion rate did not change after oral administration of budralazine (10 mg/kg and 100 mg/kg/day, p.o.; for 7 days). However, significant increase in urinary aldosterone excretion rate was demonstrated. Both plasma A II and NE concentrations tended to increase after oral administration of budralazine (100 mg/kg/day). Plasma AVP and 5-HT concentrations were not influenced by budralazine. These findings suggest that budralazine acts on renin angiotensin aldosterone system as compared to that in the sympathetic nervous system.

Aldosterone↗
PubMed1985

Stimulation of the microsomal acyl-CoA: cholesterol acyltransferase by high-cholesterol diets in rabbit liver and small intestine.

Modulation of acyl-CoA: cholesterol acyltransferase (ACAT; EC 2.3.1.26) activity by different diets or coincubation with serum lipoproteins was studied in rabbit liver and intestinal mucosa microsomal preparations. The total amount of cholesterol in the serum lipoproteins, hepatic cells and small intestine mucosa changes in response to changes in cholesterol dietary period. The concentration of either esterified or unesterified cholesterol present in liver and small intestine microsomes remains within a relatively narrow range, whereas the activity of ACAT increased remarkably. In order to clarify the mechanism by which the activity of ACAT was stimulated, liver microsomes of normal rabbits were preincubated with lipoproteins. All lipoprotein fractions stimulated microsomal ACAT activity, among which the intermediate lipoprotein (IDL) showed most effective. These results suggest that ACAT activity in microsomes was affected not only by the amount of microsomal cholesterol, but also by an extra-microsomal soluble protein such as lipoproteins.

Animals↗
PubMed1984

Forskolin suppresses seizures induced by pentylenetetrazol in mice.

Forskolin, an adenylate cyclase activator when injected s.c. (1 mg/kg) in mice, caused an elevation of cAMP in the forebrain and cerebellum of up to 170% and 130%, respectively. The treatment was found to prevent seizures induced by pentylenetetrazol. This suppression had subsided 30 min after the injection, when cAMP level was again normal in the cerebellum but still elevated in the forebrain.

Adenylyl Cyclases↗
PubMed1984

Angiostrongylus cantonensis: paralysis due to avermectin B1a and ivermectin.

Paralysis due to avermectin B1a and ivermectin of Angiostrongylus cantonensis was compared to that of phenylephrine (an alpha-adrenergic agonist) and strychnine (a cholinergic inhibitor). The paralyzing action of ivermectin (2.5 X 10(-9) g/ml) was inhibited by the single, simultaneous addition of picrotoxin (3 X 10(-5) M), whereas the effect of the drug (2.5 X 10(-7) g/ml) was reversed only when picrotoxin was given with cholinergic spasmogens such as pyrantel and eserine. Bicuculline (3 X 10(-5) M) had a similar antagonistic effect for picrotoxin, but bicuculline was less effective. The paralyzing action of avermectin B1a (3.6 X 10(-14) M, 3.0 X 10(-14) g/ml) was antagonized only when picrotoxin was given with cholinergic spasmogens such as pyrantel, eserine, and N-methylcytisine (N-MC), or alpha-adrenergic antagonists such as phentolamine and dibenamine. On the other hand, the paralyzing action of strychnine (3 X 10(-6) M) or phenylephrine (3 X 10(-5) M) was relatively uninfluenced by picrotoxin, but was antagonized by pyrantel and N-MC or dibenamine. These results suggest that a gabergic mechanism is involved in the paralyzing action of ivermectin, as well as avermectin B1a, in A. cantonensis.

Adrenergic alpha-Antagonists↗
PubMed1984

Existence of suppressor cells in the spleen of allogeneic and syngeneic primiparous pregnant mice.

Mixed lymphocyte reaction (MLR) and in vitro induction of cytolytic cells against alloantigens were investigated using spleen cells from primiparous mice mated allogeneically or syngeneically. One-way MLR was reduced significantly in degree not only in allogeneic but also in syngeneic pregnant mice. MLR of virgin spleen cells was suppressed when mitomycin C-treated spleen cells taken from syngeneic or allogeneic pregnant mice were added as regulator cells. These suppressive effects disappeared when regulator cells were treated with anti-Thy 1 or anti-Lyt 2 serum plus complement. Generation of cytotoxic lymphocytes from syngeneic or allogeneic pregnancy spleen cells in MLR was depressed compared with that from virgin spleen cells. The addition of pregnancy spleen cells to MLR suppressed in vitro generation of cytotoxic lymphocytes from virgin spleen cells. These results indicated that reduction of in vitro cellular responses of pregnancy spleen cells was due to suppressor cells in the spleens. These cells suppressed non-specifically the reactions to alloantigens and could be detected both in allogeneic and syngeneic primiparous pregnancies.

Animals↗
PubMed1984

Endogenous indole-3-acetic Acid in the stem of tobacco in relation to flower neoformation as measured by mass spectroscopic assay.

The contents of free indole-3-acetic acid (IAA) and alkali-labile, conjugated IAA were measured in relation to a ;floral gradient' present in epidermis and subepidermis tissues of flowering plants of Nicotiana tabacum by capillary gas-chromatographic spectrometric analysis by selected ion monitoring (GC-SIM-MS) using 2,4,5,6,7-penta deutero IA ((2)H(5)-IAA) as an internal standard. In floral axes, floral branches and stems with floral branches, free IAA levels (dry weight) were 387, 253, and 417 nanograms, and bound IAA levels were 99, 1089, and 268 nanograms. In vegetative tissue of the first plus second internodes (measured from top), and of the 11th to 13th internodes, free IAA levels were 826 and 500 nanograms, and bound IAA levels were 1421 and 286 nanograms, respectively. Since flower-forming ability of excised cells from the epidermis and subepidermis shows a gradient in an in vitro system, but levels of IAA in these tissues do not, there thus appears to be no correlation between flower-forming ability (in vitro) and endogenous IAA levels (at the time of excision) in tobacco stem tissues.

Journal Article↗
PubMed1984

Immunohistochemical appearance of calmodulin in the developing brain: a comparison with neuron specific enolase.

Calmodulin is a small, acidic, calcium-binding protein thought to regulate many cellular functions. In the brain of the adult mouse, calmodulin was found immunohistochemically to localize mainly in the neurons. In the developing brain, the immunoreactivity to anti-calmodulin antibody appeared early in the cells in the low brain stem but late in the cerebral cortex, hippocampus, and cerebellum, except for the deep cerebellar nuclei. The cells in the major proliferative layer present during early development, such as the matrix cells in the cerebral cortex and the cells in the external granular layer in the cerebellum, did not show the immunoreactivity. In the cerebral cortex, the migrating cells and the cells in the cortical plate were also negative while the deep cortical cells, which had probably settled in their final position, became positive. The comparison of these results with the immunohistochemical appearance of neuron specific enolase, a characteristic protein in the brain, suggested that calmodulin appeared with some maturation of the neurons as neuron specific enolase.

Animals↗
PubMed1984

Identification and quantification of the left-to-right shunt in adults with atrial septal defect by two-dimensional negative contrast echocardiography.

Negative contrast echocardiograms of two-dimensional long axis view of the right ventricle and the atrium were compared with the results of cardiac catheterization and surgical findings in 33 adults with atrial septal defects. Contrast echocardiographic evidence of atrial communication was noted in 30 cases (91%), and, in 28 cases, the negative contrast during one cardiac cycle could be measured. This could be applied even in cases with a small left-to-right shunt or with Eisenmenger's syndrome. No obvious difference in the behavior of negative contrast was documented among the cases with distinct types of septal defects. The maximum negative contrast area and the duration of appearance in one cardiac cycle were related to the pulmonary-to-systemic flow ratio. However, a significant overlap and considerable effects of transducer angulation on measurements were documented. Thus, negative contrast echocardiography may be widely used for the noninvasive identification of a left-to-right shunt in atrial septal defects. However, the method is not appropriate for a precise quantification of three-dimensional shunt flow.

Adolescent↗
PubMed1984

Gallium induces reduction of negative charge of the cell membrane and redifferentiation of cancer cells.

In order to obtain an insight into the mechanism of experimentally induced redifferentiation of cancer cells, changes in negative charge of the cell membrane were examined by cytopherometry following in vitro exposure of the cancer cells to non-radioactive gallium. Reduction of the negative charge was observed at 24 hr and later. The gallium ions must have neutralized the negative charge of the cancer cell membrane to the normal range. This event may contribute in some way to cancer cell redifferentiation.

Adenocarcinoma↗
PubMed1984

Ultrastructural study of the effect of acute hyper- and hypotension on the stria vascularis and spiral ligament.

The effects of acute hyper- and hypotension on the stria vascularis and spiral ligament of the rat were studied electron microscopically with the horseradish peroxidase (HRP) tracer method. Acute hypertension was induced by i.v. infusion of methoxamine chloride (Mexan), and acute hypotension by i.v. infusion of a ganglion-blocker (Arfonad) and by venesection. In both acute hyper- and hypotensive experiments, a large amount of leaked HRP spread into the intercellular spaces until it was stopped by tight junctions bordering the stria vascularis. The stria capillary endothelium displayed a dense distribution of labelled vesicles, which suggests increased vesicular transport. There was no extravasation of HRP from capillaries in the spiral ligament, despite the presence of some labelled pinocytotic vesicles. The present paper deals with the discovery of enhanced capillary permeability of the stria vascularis under acute hyper- and hypotension, and makes comparison between acute hyper- and hypotension in order to define the function of the stria vascularis.

Animals↗
PubMed1984

Polyester wax embedding and sectioning technique for immunohistochemistry.

We have developed a method useful for immunohistochemical studies by combining tissue fixation with buffered neutral formalin and polyester wax embedding. Buffered neutral formalin fixation preserves cell and tissue fine structure, and also the antigenicity of unstable enzymes. Polyester wax embedding makes possible thin serial sections of various tissues and preserves antigenicities for at least 6 months. We have demonstrated using this technique the localization of alpha-amylase in mouse salivary gland, parietal-cell specific antigen in mouse glandular stomach, and DNA polymerase alpha and beta in chick tissue.

Animals↗
PubMed1984

[2 cases of granular cell tumor of the breast].

Two cases of granular cell tumor of the breast are reported. This disease is uncommon; only 54 cases have been reported so far. Clinically, it resembled breast cancer. Its palpation is solid. It sometimes has a dimpling sign, and, on occasion, the X-ray diagnosis shows a spicule. Pathologically, the tumor has a tendency to infiltration. The tumor cells contain granules of different sizes. Straited muscles, mesenchymal cells and Schwann's cells have been pointed out as possible roigins of the tumor. It has also been explained as histiocytic granuloma. A decisive theory about its organic development, however, has yet to be established.

Adult↗