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Biomedical subjects

M Sakka

Publications and source records attributed to M Sakka.

At least 55 records · Page 3Linked to original sources
PubMed1978

Pedigree analysis of the hypothermal recovery of the effects of gamma-rays on hepatoma cells.

Cultured hepatoma cells of the rat were irradiated with 400 to 900 R of gamma-rays. They were kept at room temperature for several hours before transferred to normal culture condition at 37 degrees C. The pedigree analysis of growing cells recorded by cinematography indicated a variety of lethal and non-lethal recovery events such as a decrease in lethal branch formation and an increase in locomotion. The so-called repair immediately after irradiation has many facets depending upon dose and duration of the low temperature treatment.

Animals↗
PubMed1976

Kinetics of microcolonies of cultured mammalian cells after bleomycin treatment.

Cultured ascites hepatoma cells of the rat were treated with 400 to 900 R of gamma rays or 25 to 100 mug/ml of bleomycin for 30 min. The cells were followed up for 133 hr and the pedigrees were analyzed. Gamma rays and 2 low doses of bleomycin gave rise to typical reproductive death in generations 1 and 2 but 100 mug of the drug produced frequent interphase death. Natural death and induced death did not follow statistical randomness suggesting an existence of weak and sensitive clones in a population.

Bleomycin↗
PubMed1975

Kinetics of microcolonies of cultured mammalian cells after gamma irradiation.

The time of cell destruction was determined by pedigree analysis before macrocolony formation immediately after irradiation of gamma rays (400-900 R) in cultured rat hepatoma cells. Cell destruction occurred and was distributed to various generations in proportion to dose. The loss of reproductive integrity resulting in an extinction of a clone was limited in earlier generations, 0 to 2, but the loss of reproductive capacity was observed in later generations. It seems probable that two types of loss of reproduction come from random transmission of lethal signals produced in parent cells irrespective of the pattern of pedigrees.

Animals↗
PubMed1974

The effect of bleomycin and its combined effect with radiation on murine squamous carcinoma treated in vivo.

Many previous reports testify to the effectiveness of bleomycin as a drug for the clinical treatment of squamous carcinomata, and some radiotherapists have considered the possibility that an improved therapeutic ratio might be obtained by combined treatment with bleomycin and radiation. This consideration has been stimulated by recent reports of the effects of combined treatment with bleomycin using mammalian cells in vitro. Experiments are described here in which a murine squamous carcinoma has been used to obtain a survival curve for cells of tumours treated in vivo with single doses of bleomycin alone or in combination with radiation given before or after the drug. The survival curve for drug alone was a multicomponent curve with D(0) values of 0·1, 0·75 and 2·7 mg/kg. However, the results of the experiments with combined treatment showed no evidence of potentiation.

Animals↗