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Biomedical subjects

M Sakai

Publications and source records attributed to M Sakai.

At least 739 records · Page 41Linked to original sources
PubMed1984

Quantitative measurement of portal blood flow in patients with chronic liver disease using an ultrasonic Duplex system consisting of a pulsed Doppler flowmeter and B-mode electroscanner.

Portal blood flow (PBF) can be measured quantitatively using a B-mode combined pulsed Doppler (BCD) system. This system combines a real time B-mode linear type electroscanner and a pulsed Doppler (D-mode) flowmeter. Since both modes are displayed in realtime, Doppler blood flow signals can be retrieved at will from any depth. The blood flow velocity determined by the Doppler spectrogram and the vascular cross-sectional area measured from the B-mode tomographic image enables the quantitative calculation of blood flow volume. Using this system, PBF was measured quantitatively in 88 healthy adults, 54 patients with chronic hepatitis, 65 with cirrhosis of the liver, 27 with primary hepatoma and 12 with idiopathic portal hypertension (IPH). Results of PBF volume measurement were as follows: 889 +/- 284 ml/min (mean +/- S.D.) for healthy adults, 851 +/- 237 ml/min for patients with chronic hepatitis, 870 +/- 289 ml/min for cirrhosis of the liver, 966 +/- 375 ml/min for primary hepatoma and 1,047 +/- 381 ml/min for IPH. These preliminary results demonstrated that this ultrasonic Duplex system is clinically useful to determine the quantitative amount of PBF.

Carcinoma, Hepatocellular↗
PubMed1984

Tandem arrangement of the albumin and alpha-fetoprotein genes in the human genome.

A genomic clone containing human albumin mRNA sequences was isolated from a lambda phage gene library derived from a human hepatoma cell line that produces alpha-fetoprotein (AFP) and albumin. This clone was also found to hybridize with the 5'-flanking region of the human AFP gene. Restriction mapping and nucleotide sequencing revealed that the albumin and AFP genes are present in tandem, in the same transcriptional orientation, with the albumin gene 14.5 kb upstream of the AFP gene. We have also isolated a genomic clone carrying both the albumin and AFP gene sequences from human fibroblasts, which produce neither AFP nor albumin. This DNA showed a restriction map that was indistinguishable from that of the clone obtained from the hepatoma described above, demonstrating that no gross rearrangements of the intergenic DNA sequence are involved in control of expression of the AFP and albumin genes.

Bacteriophage lambda↗
PubMed1984

[Indication of aorto-coronary bypass surgery for the infarcted myocardium: assessment using exercise T1-201 myocardial imagings].

Using exercise thallium-201 myocardial imagings, 23 patients with previous myocardial infarction were evaluated before and after aorto-coronary bypass graft (ACBG) surgery to assess the indication of the operation for the infarcted myocardium. The patients were categorized as group I with regional hypoperfusion and redistribution in the infarcted site (11 patients), and group II with regional hypoperfusion without redistribution (12 patients). Changes in the exercise tolerance before and after ACBG: By the criteria of Canadian Cardiovascular Society, clinical symptoms improved in nine of 11 patients (82%) in group I, and eight of 12 patients (67%) in group II. Pressure rate product improved significantly in group I (201 +/- 28% to 219 +/- 21% p less than 0.05), whereas it showed no significant change in group II (200 +/- 44% to 203 +/- 35%). Changes in the global left ventricular ejection fraction before and after ACBG: There was a significant increase in group I (63 +/- 11% to 68 +/- 9% p less than 0.02), whereas no significant change in group II (53 +/- 16% to 54 +/- 14%). Changes in myocardial perfusion in the infarcted segment before and after ACBG: Group I showed an increase in myocardial perfusion in all of the 11 (82%) except two patients with the occluded graft, and group II showed an increase in only two of the 12 patients (17%). Changes in wall motion abnormality in the infarcted segment before and after ACBG: Improvement of the wall motion abnormality was observed in eight of the 11 patients (73%) in group I, whereas only one of the 12 patients (8%) in group II. It is suggested that preoperative redistribution of the infarcted site in the delayed image suggests myocardial viability, and allows us to expect the postoperative improvement of myocardial hypoperfusion and wall motion abnormality.

Adult↗