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Biomedical subjects

M Sageshima

Publications and source records attributed to M Sageshima.

At least 37 records · Page 2Linked to original sources
PubMed1990

Development of binucleated myocytes in normal and hypertrophied human hearts.

To clarify the development of binucleated cardiac myocytes (BCMs) in human hearts, lengths for BCMs and mononucleated cardiac myocytes (MCMs) were histologically measured and compared in 49 hearts weighing 13-730 g. The mean cell length was significantly longer in BCMs. The growth rate of cell length relative to the heart weight in BCMs and MCMs was almost identical and less than 0.333. The scattering of different lengths of BCMs and MCMs was much greater in adults, suggesting that each myocyte develops at a different rate. Short MCMs of 37.5 microns in hypertrophied hearts suggested that some BCMs might divide into two. In adult hearts, binucleation might arise, because BCMs with internuclear distance under 2.5 microns were found. The mean half of the sum of nuclear lengths of BCMs was shorter than the mean nuclear length of MCMs. They were largely constant in hearts weighing under 500 g but increased in larger hearts.

Adolescent↗
PubMed1989

Immature alveolar/blood barrier and low disaturated phosphatidylcholine in fetal lung after intrauterine exposure to O,O,S-trimethylphosphorothioate.

Intrauterine exposure to the potent lung toxicant OOS-TMP was found to result in neonatal lethality attributed to immature lungs (Koizumi et al. 1988). The present study was initiated to investigate biological/pathological profiles of such pulmonary immaturity. OOS-TMP was given p.o. to five pregnant female Sprague-Dawley rats on gestation day (G) 19 at 2.5, 7 or 20 mg/kg. Control (N = 6) or pair-fed dams (N = 5: pair-fed to 20 mg/kg dams) received 2 ml/kg corn oil. On G 22, fetuses were delivered by Cesarean section. The biochemical maturity of lungs was assessed by glycogen content and production of disaturated phosphatidylcholine (DSPC), a major component of pulmonary surfactant. Mean DSPC content was significantly lower in fetuses from dams dosed at 7 or 20 mg/kg while mean glycogen concentration, in contrast, was 3- to 6-fold higher in those fetuses than fetuses from control or pair-fed dams. Pathological examination revealed that in fetuses delivered from dams dosed at 7 mg/kg or 20 mg/kg, glycogen-rich cuboidal epithelial cells completely covered the terminal air space and alveolar/blood barriers stayed at the poorly developed stage. The stage of the pulmonary development in those fetuses was similar to those in fetuses on G19. Therefore it was concluded that intrauterine exposure to OOS-TMP delayed pulmonary development, thereby causing respiratory failure after birth.

Animals↗
PubMed1989

Endocardium of the left ventricle in volume-loaded canine heart. A histological and ultrastructural study.

To investigate the effects of volume loading of the heart, the endocardium was studied histologically and ultrastructurally. Thirteen adult female beagle dogs were used. An arterio-venous shunt was constructed between the right common carotid artery and the right external jugular vein in nine animals to induce a volume load. Four animals were used as controls. All were kept for 6-12 months. Heart weight, relative heart weight (heart weight/body weight), cardiac output index, stroke volume index and volumes of both ventricles in the experimental animals were significantly larger than in the controls. Shunted blood volume was significantly correlated with heart weight. The endocardium of the left ventricle in the experimental animals showed elastofibrosis and was significantly thicker than in the controls. In 5 hearts, it was more than 20 microns thick and its endothelial cells showed many long microvilli with a very thick basement membrane (1.5-2.0 microns). The thickness of the endocardium was significantly correlated with the heart weight, relative heart weight and cardiac output index, within 1%, 5% and 5% risk, respectively. These endocardial changes were thought to be induced by hemodynamic changes in the left ventricle of the volume-loaded heart, probably being correlated with changes in cardiac function and morphology.

Animals↗
PubMed1988

Primary malignant astrocytoma of the cerebellum in children. Report of a case with electron microscopic and cytofluorometric DNA evaluation.

The case of a 1-year-old girl with primary malignant astrocytoma of the cerebellum is presented with special reference to ultramicroscopic study and cytofluorometric DNA analysis. The routine light microscopic study was not pathognomonic because of the immaturity of the composing cells. The glial fibrillary microfilaments characteristic of astroglial cells were confirmed by means of electron microscopy. Cytofluorometrically, the DNA histogram of this case revealed a prominent peak in the 4C region, with considerable dispersion to the 8C region, indicating the polyploidic and heterogeneic pattern of malignant tumors.

Astrocytoma↗
PubMed1987

Classification of cardiac rupture complicated in myocardial infarction. Pathological study of 32 cases.

Thirty of 32 cases with cardiac rupture (CR) complications in the myocardial infarction were found out of a total of 91 cases of acute myocardial infarction (AMI). The mean age of the ruptured group in females was significantly younger than that of the non-ruptured group. Twenty-one cases showed free wall rupture of the left ventricle, six perforation of ventricular septum and three double rupture. All cardiac ruptures occurred in cases of transmural infarction. The age of AMI was histologically estimated. Nine cases complicated within the first 24 hours of AMI showed rupture of the left anterior wall. CR in the periphery within the infarct occurred at any time during the first week after onset, and cases of the central rupture were increased in number after the 3rd day of AMI. Pathologic findings indicated that elevated wall tension was considered to be most closely related to the cause of CR. Thirty two cases of CR were classified into three types: (1) blowout type, (2) hemorrhagic dissecting type, and (3) thinning-with-rupture type. Hemorrhagic dissecting type was characterized by multiple endocardial ulcers and fissure canals extending from the ulcer with hemorrhage in the surrounding myocardium. Complex fissure was seen in two cases of this type.

Aged↗
PubMed1987

Immature cerebellar astrocytoma in an infant.

A case of immature cerebellar astrocytoma in a 1 year and 4 months-old girl was described. The tumor consisted mainly of closely packed relatively uniform round cells with prominent intervening capillaries. Although tumor cells showed negative GFA (glial fibrillary acidic protein) immunoreactivity, demonstration of occasional bundles of 10 nm intermediate filaments (glial filaments) in cytoplasm on electron microscopy confirmed the diagnosis of astrocytoma. Differential diagnosis to hemangioblastoma, astroblastoma, and primitive neuroectodermal tumor was discussed.

Astrocytoma↗
PubMed1987

Flow loaded canine carotid artery. II. Ultrastructural changes in the subendothelial layer.

To study the subendothelial layer of the flow loaded arteries, blood flow changes were produced by constructing an arterio-venous shunt between the common carotid artery and the external jugular vein using eleven adult beagle dogs. One week after operation, the subendothelial layer of the flow loaded canine carotid arteries was observed with a transmission electron microscope. In the arteries loaded by highly elevated blood flow, the subendothelial layer showed thick subendothelial space (300 to 500 nm) with multilayered basement membrane. The microfilaments and microfibrils increased significantly. There were no collagen fibers. Spindle to cylindrical elastic fibers appeared in the luminal margin of the internal elastic lamina. On the other hand, in the control arteries, the subendothelial layer was thin (about 50 nm) with scanty basement-membrane-like material. There were a few microfilaments and microfibrils, but no collagen fibers. The luminal margin of the internal elastic lamina was smooth. It is suggested that these are the wall shear stress dependent subendothelial changes, which would be partly due to the increased protein synthesis by the endothelial cells stimulated by the wall shear stress and be partly due to the wall shear dependent mechanical stress transmitted from the endothelial surface.

Animals↗
PubMed1987

Membranous lipodystrophy. Light and electron microscopic study of a biopsy case.

Membranous lipodystrophy is a peculiar systemic disease almost exclusively found in Japan and Scandinavia and characterized by formation of numerous membranous structures in the adipose tissue of bone marrow and soft tissue. Light and electron microscopic study of a biopsy case of the disease was presented. The membranous structures consisted of membrane and inner space on both light and electron microscopic study. The membrane consisted of two kinds of layers, dense and amorphous. The dense layer was positive for lipid stainings. The amorphous layer did not show any definite lipid constituents and was supposed to consist of an amorphous substance derived from abnormal fat metabolism of diseased fat cells, degenerative cytoplasms of the diseased cells, and/or stromal cells. It probably also had some stromal substance alteration due to membranolipodystrophic change. Accumulation of the amorphous layer in the interstitium leads to degeneration and disappearance of adjacent fat cells. This process may play a role in further advance of membranous structures.

Adipose Tissue↗
PubMed1986

Flow loaded canine carotid artery. I. A morphometric study of microfilament bundles in endothelial cells.

To estimate microfilament bundles in the endothelial cells of flow loaded arteries quantitatively, blood flow change was produced in the common carotid arteries of eleven female beagle dogs (1-2 years of age, 8-10 kg) employing the arteriovenous shunt method between the common carotid artery and external jugular vein. After 1 week, the amount of microfilament bundles was measured with a point counting method on transmission electron microscopical photographs. In the arteries loaded by highly elevated blood flow, the average thickness of microfilament bundles increased over 0.1 mu m (non-operative control: 0.01-0.02 mu m) and the average longitudinal cut area of a microfilament bundle grew over 0.7 mu m2 (non-operative control: 0.2 mu m2). The number of bundles increased over 1.0 piece (non-operative control: 0.2 pieces) per unit length (mu m) in transverse section and increased over 0.5 pieces (non-operative control: 0.2 pieces) in longitudinal section. The average thickness of the bundles significantly correlated to the blood flow increase ratio (the ratio of the blood flow rate of the final measurement to that before anastomosis). We consider that the microfilament bundles are the structure developed to combat the wall shear stress induced by the elevated blood flow.

Actin Cytoskeleton↗
PubMed1985

[Liposarcomatous tumor emboli to the left iliac artery growing intraluminally into the femoral artery. A report of an operative and autopsy case].

A 74-year-old female patient with a past history of the locally recurrent tumor of the liposarcoma in the right thigh over 20 years ago received an emergency vascular operation to relieve severe ischemic symptoms in the left leg. The left femoral artery was pulseless and was filled with several long, translucent and cord-like tumors which resembled the hands of a cuttlefish. The tumors were easily removed except its root adhering to the left common iliac artery. A crossover femoro-femoral artery bypass was carried out. On the 24th postoperative day she died suddenly. The autopsy findings were as follows: A hen egg-sized tumor occupied almost whole space of the left atrium, and connected with the metastatic right lung tumor via the pulmonary vein. The sudden death was probably due to the abrupt obstruction of the mitral valve by the tumor in the left atrium. The residual tumor was noted in the left common iliac artery, but its adventitia was not involved. The pathohistological diagnosis of each tumor was same, well differentiated liposarcoma. The tumors which occluded the left iliofemoral artery must have originated from the tumor in the left atrium in form of the tumor embolism, lodged in the left common iliac artery, grown intraluminally into external iliac and femoral artery, and finally caused ischemia of the left leg.

Aged↗
PubMed1984

Progeria with cardiac hypertrophy and review of 12 autopsy cases in the literature.

An autopsy of an 11 years 5 months old boy with progeria is presented. Hyaluronuria, low growth rate of skin fibroblasts, decreased number of T-cells in lymphocyte subpopulation, increase of anti DNA antibody, and antibody for microsome of thyroid gland were detected clinically. He had suffered an attack of cerebral infarct and died of congestive cardiac failure. Pathologic findings were scleroderma-like skin atrophy, moderate arteriosclerosis of the aorta and great arteries, severely narrowed coronary sclerosis with an extensive subendocardial fibrosis, a large left cerebral infarct with pin-holed stenosis of both internal carotid arteries, cortical atrophy of thymus, and atrophic lymph nodes. The cardiac muscle fibers were slightly hypertrophied and measured 15.5 +/- 2.8 microns in diameter. Histologic findings suggest that increasing of collagen in the connective tissue may play an important role in progeria. Further study of metabolic disturbance in the connective tissue of progeria is necessary.

Autopsy↗
PubMed1983

[Pathological and clinical studies on primary aldosteronism].

In the surgical treatment of patients with primary aldosteronism, differentiation of idiopathic hyperaldosteronism due to bilateral adrenal hyperplasia should be kept in mind. Controversies exist, on the other hand, concerning the diagnosis and management of multiple nodular hyperplasia which often coxists with adrenal adenoma. We studied correlations between pathological findings and clinical diagnosis on 13 patients with primary aldosteronism who were operated on during the 19 year period from 1963 to 1981, and the results were as follows; (1) Histologically, adrenal glands of these patients were classified into three subgroups, namely i) adrenocortical adenoma alone, ii) adrenocortical adenoma associated with multiple nodular hyperplasia, and iii) nodules of multiple nodular hyperplasia alone. (2) Clinically, the latter two subgroups showed increase in plasma renin activity following the combination test of volume depletion and spironolactone administration. From these results it is suggested that even in patients with primary aldosteronism multiple nodular hyperplasia of the adrenal cortex can coexist together with adrenal adenoma, and that coexistence of this hyperplasia may modify some clinical features of the disease.

Adenoma↗
PubMed1980

[Case of cystic adventitial degeneration of the brachial artery].

Cystic degenerative disease of the brachial artery, which caused acute and total occlusion of the artery was encountered in a 51 years old male patient. At the exploration of the brachial artery at the lower border of the axilla, an anatomical variation, high division of the superficial brachial artery, was observed. At the bifurcation of the artery, a cystic degenerative change was detected and was judged to be the cause of the arterial occlusion. He was treated successfully with partial resection of the arterial wall containing the cyst, followed by autologous vein patch plasty.

Arm↗
PubMed1978

Long-term prognosis on recurrence of seizures among children with epilepsy after drug withdrawal-elimination.

The long-term prognosis of 110 children after withdrawal-elimination of the drugs on the basis of our rigid criteria was analyzed. The 12 cases (10.9%) of 110 patients experienced recurrences, which occurred between three months to three years after drug elimination, but there were no recurrences later than three years to 10 years after drug elimination. The asymmetry in the EEG during the first two to three years after drug elimination appeared in 6.4% and seizure discharge appeared in 17.1%. But no asymmetry nor seizure discharge was found in EEG later than three years after drug elimination.

Anticonvulsants↗
PubMedDate not supplied

Orbital hemangiopericytoma: CT, MR, and angiographic findings.

A case of orbital hemangiopericytoma is reported in a 20-yr-old man presenting with decreased vision and painless proptosis of the left eye. This rare orbital tumor is reviewed in the literature, and in particular the neuroradiological findings, including those of computerized tomography (CT), magnetic resonance imaging (MRI), and angiography are detailed in the presented case. MRI was found to be useful both in delineating precise anatomical relationships between the optic nerve and the tumor, and in differentiating from cavernous hemangiomas, the most common vascular tumors of the orbit, with the combination of CT and angiography.

Adult↗
PubMedDate not supplied

Intraosseous ganglion of the metatarsal bone.

We describe a rare case of intraosseous ganglion arising in the metatarsal bone. Radiographs revealed an osteolytic lesion with a fracture in the third metatarsal bone. A biopsied specimen exhibited hyaline fibrous tissue with marked myxoid change. Gadolinium-enhanced MRI, which revealed the network-like enhancement of the rim of the lesion and polycystic lesions adjacent to the joint, was helpful in making a diagnosis of intraosseous ganglion.

Biopsy↗